일반 및 동물복지농장 돼지의 혈액 및 근육 내스트레스 인자 비교
Comparison of Stress Indicators in Blood and Muscle of Pigs in Conventional and Animal Welfare Farms
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Intensive farming methods that do not guarantee animal welfare can induce stress in pigs. Stress, in turn, can reduce their disease resistance and influence their hormones and metabolites in such a manner that productivity is negatively affected. This study was conducted to compare the stress related factors and blood characteristics of pigs raised on conventional farms and those raised on animal welfare farms. We measured the levels of cortisol, epinephrine and norepinephrine, biochemical parameters in blood and glycogen, L-lactate and heat shock protein 70 (HSP70) in muscle, as physiological markers of indicating the stress in conventional farm pigs (Control, n=10) and animal welfare farm pigs (Welfare, n=10). We found that there was a significant difference in the albumin-globulin ratio (A/G ratio), as well as the albumin (ALB), blood urea nitrogen (BUN) and aspartate aminotransferase (AST) levels between the two farms. Epinephrine was significantly higher in conventional farm, while level of norepinephrine was higher in animal welfare farm. There was no significant difference in cortisol, which is known as a stress hormone, across the two groups of farms. Muscular glycogen content was significantly high in animal welfare farm pigs. While L-lactate tended to be low in the animal welfare farm pigs, the difference between them and the conventional farm cohorts was not significant. HSP70 showed high levels of expression in conventional farm. Thus, we suggested that blood parameter results showed a stress response in the livers of conventional farm, and that catecholamine hormones, glycogen, L-lactate and HSP70 can be used as physiological factors of assessing animal welfare.
Abstract
Intensive farming methods that do not guarantee animal welfare can induce stress in pigs. Stress, in turn, can reduce their disease resistance and influence their hormones and metabolites in such a manner that productivity is negatively affected. This study was conducted to compare the stress related factors and blood characteristics of pigs raised on conventional farms and those raised on animal welfare farms. We measured the levels of cortisol, epinephrine and norepinephrine, biochemical parameters in blood and glycogen, L-lactate and heat shock protein 70 (HSP70) in muscle, as physiological markers of indicating the stress in conventional farm pigs (Control, n=10) and animal welfare farm pigs (Welfare, n=10). We found that there was a significant difference in the albumin-globulin ratio (A/G ratio), as well as the albumin (ALB), blood urea nitrogen (BUN) and aspartate aminotransferase (AST) levels between the two farms. Epinephrine was significantly higher in conventional farm, while level of norepinephrine was higher in animal welfare farm. There was no significant difference in cortisol, which is known as a stress hormone, across the two groups of farms. Muscular glycogen content was significantly high in animal welfare farm pigs. While L-lactate tended to be low in the animal welfare farm pigs, the difference between them and the conventional farm cohorts was not significant. HSP70 showed high levels of expression in conventional farm. Thus, we suggested that blood parameter results showed a stress response in the livers of conventional farm, and that catecholamine hormones, glycogen, L-lactate and HSP70 can be used as physiological factors of assessing animal welfare.
참고문헌 (53)
- Adhihetty, P. J, "Creatine and its potential therapeutic value for targeting cellular energy impairment in neurodegenerative diseases", Neuromolecular Med, 10, pp.275~290 (2008)
- Adzitey, F, "Pale soft exudative (PSE) and dark firm dry (DFD) meats: causes and measures to reduce these incidences-a mini review", Int. Food Res. J, 18 (2011)
- Aradom, S, "Effect of transport times on welfare of pigs", J. Agric. Sci, 2, pp.544~ (2012)
- Barnett, J, "The effects of pregnancy and parity number on behavioural and physiological responses related to the welfare status of individual and group-housed pigs", Appl, 17, pp.229~243 (1987)
- Bliss, E. L, "Metabolism of norepinephrine, serotonin and dopamine in rat brain with stress", J. Pharmacol. Exp. Ther, 164, pp.122~134 (1968)
- Bliss, E. L, "Brain amines and emotional stress", J. Psychiatr. Re, 4, pp.189~198 (1966)
- Bowker, B, "Muscle metabolism and pse pork", J. Anim. Sci, 79, pp.1~8 (2000)
- Breinekova, K, "Markers of acute stress in pigs", Physiol. Res, 56, pp.323~ (2007)
- Briggeman, B. C, "Preferences for fairness and equity in the food system", Eur. Rev. Agric, 38, pp.1~29 (2011)
- Broom, D, "Effects of disease on farm animal welfare", Acta. Vet. Brn, 71, pp.133~136 (2002)
- Broom, D. M, "Animal welfare: an aspect of care, sustainability, and food quality required by the public", J. Vet, 37, pp.83~88 (2010)
- Burchfield, S. R, "The stress response: a new perspective", Psychosom. Med, 41, pp.661~672 (1979)
- Candiani, D, "A combination of behavioral and physiological indicators for assessing pig welfare on the farm", J. Appl. Anim. Welf. Sci, 11, pp.1~13 (2008)
- Chida, Y, "Does stress exacerbate liver diseases", J. Gastroenterol. Hepatol, 21, pp.202~208 (2006)
- 조남욱, "화재로 인한 불화수소(HF)가스에 노출된 마우스의 혈액생화학분석 및MDA분석을 통한 세포손상에 관한 연구", 한국가스학회지, 17(6), pp.58~66 (2013), 한국가스학회[DOI]
- 추금숙, "전북 익산지역 양돈장에서 돼지 생식기 호흡기 증후군, 서코바이러스-2 및 마이코프라즈마 폐염으 항체가 조사", 한국동물위생학회지, 30(3), pp.305~312 (2007), 한국동물위생학회
- Cronin, G, "The welfare of pigs in two farrowing", lactation environments: cortisol responses of sows, 1991, pp.32, 117~127
- Currie, R. W, "Characterization of the synthesis and accumulation of a 71-kilodalton protein induced in rat tissues after hyperthermia", Can. J. Biochem. Cell Bio, 61, pp.438~446 (1983)
- Dalin, A, "The effect of transport stress on plasma levels of catecholamines, cortisol, corticosteroid-binding globulin, blood cell count, and lymphocyte proliferation in pigs", Acta Vet. Scand, 34, pp.59~ (1993)
- De Maio, A, "Heat shock proteins: facts, thoughts, and dreams", Shock, 11, pp.1~12 (1999)
- Duncan, I. J, "Science-based assessment of animal welfare: farm animals", Rev. Sci. Tech. Oie, 24, pp.483~ (2005)
- Etim, N. N, "Stress and animal welfare: an uneasy relationship", Eur. J. Med. Sci, 2 (2014)
- Fâbrega, E, "Effects of halothane gene and pre-slaughter treatment on meat quality and welfare from two pig crosses", Meat Sci, 62, pp.463~472 (2002)
- Fernandez, X, "A review of the causes of variation in muscle glycogen content and ultimate ph in pigs", J. Muscle Foods, 2, pp.209~235 (1991)
- Foury, A, "Stress hormones, carcass composition and meat quality in large white× duroc pigs", Meat Sci, 69, pp.703~707 (2005)
- Goldstein, D. S, "Stress, catecholamines, and cardiovascular disease" (1995), Oxford University Press
- Grunert, K. G, "Consumer interest in environmental impact, safety, health and animal welfare aspects of modern pig production: results of a cross-national choice experiment", Meat Sci, 137, pp.123~129 (2018)
- Gupta, S. C, "Heat shock proteins in toxicology: how close and how far?", Life Sci, 86, pp.377~384 (2010)
- 황의경, "퀘르세틴 첨가가 고지방 및 고콜레스테롤 식이 랫드의 혈청 성분 농도의 변화에 미치는 영향", 한국임상수의학회지, 26(5), pp.413~418 (2009), 한국임상수의학회
- Ismail, H. T. H, "Effect of acute exposure to nonylphenol on biochemical, hormonal, and hematological parameters and muscle tissues residues of nile tilapia; oreochromis niloticus", Vet. World, 9, pp.616~ (2016)
- Jones, E, "Malignant hyperthermia of swine", Anesthesiology, 36, pp.42~51 (1972)
- Ju, X. H, "Heat stress upregulation of toll-like receptors 2", 4 and acute inflammatory cytokines in peripheral blood mononuclear cell (pbmc) of bama miniature pigs: an in vivo and in vitro study, 2014, pp.8, 1462~1468
- Khassaf, M, "Time course of responses of human skeletal muscle to oxidative stress induced by nondamaging exercise", J. Appl. Physiol, 90, pp.1031~1035 (2001)
- 김경아, "간기능검사의 이해와 적용", 대한내과학회지, 76(2), pp.163~168 (2009), 대한내과학회
- 김동욱, "사료 내 Quercetin의 첨가가 육계의 생산성, 혈액 생화학 특성, 혈액 내 면역글로불린과 혈액 내 항산화 인자에 미치는 영향", 한국가금학회지, 42(1), pp.33~40 (2015), 한국가금학회
- Koopmans, S. J, "Surplus dietary tryptophan reduces plasma cortisol and noradrenaline concentrations and enhances recovery after social stress in pigs", Physiol. Behav., 85, pp.469~478 (2005)
- Lachapelle, G, "Acute acidification or amiloride treatment suppresses the ability of hsp70 to inhibit heat-induced apoptosis", Apoptosis, 12, pp.1479~1488 (2007)
- Levy, B, "Effects of epinephrine and norepinephrine on hemodynamics, oxidative metabolism, and organ energetics in endotoxemic rats", Intensive Care Med, 29, pp.292~300 (2003)
- Liu, Y, "Changes in skeletal muscle heat shock proteins: pathological significance", Front. biosci, 6, pp.D12~D25 (2001)
- Martïnez-mirõ, S, "Causes, consequences and biomarkers of stress in swine: an update", BMC Vet. Res, 12, pp.171~ (2016)
- Moberg, G. P, "A model for assessing the impact of behavioral stress on domestic animals", Anim. Sci. J, 65, pp.1228~1235 (1987)
- Moberg, G. P, "The biology of animal stress: basic principles and implications for animal welfare" (2000), CABI
- Përez, M, "Effect of transport time on welfare and meat quality in pigs", Meat Sci, 61, pp.425~433 (2002)
- 박종원, "우리나라 동물복지축산의 현황과 법적 과제", 환경법과 정책, 19, pp.131~176 (2017), 비교법학연구소[DOI]
- Roth, K. A, "Epinephrine, norepinephrine, dopamine and serotonin: differential effects of acute and chronic stress on regional brain amines", Brain Res, 239, pp.417~424 (1982)
- Sepponen, K, "The inducible form of heat shock protein 70 in the serum, colon and small intestine of the pig: comparison to conventional stress markers", Vet. J, 171, pp.519~524 (2006)
- Siegel, P, "Livestock handling and transport", pp.19~29 (2000), King’s Lynn
- Sutherland, M. A, "A multidisciplinary approach to assess the welfare of weaned pigs during transport at three space allowances", J. Appl. Anim. Welf. Sci, 13, pp.237~249 (2010)
- Valros, A, "Physiological indicators of stress and meat and carcass characteristics in tail bitten slaughter pigs", Acta Vet. Scand, 55, pp.75~ (2013)
- Van Loo, E. J, "Consumers’ valuation of sustainability labels on meat", Food policy, 49, pp.137~150 (2014)
- Velarde, A, "Animal welfare towards sustainability in pork meat production", Meat Sci, 109, pp.13~17 (2015)
- Webster, A. J, "Farm animal welfare: the five freedoms and the free market", Vet. J, 161, pp.229~237 (2001)
- Zhang, M, "Variation in the expression of hsp27, αb-crystallin mrna and protein in heart and liver of pigs exposed to different transport times", Res. Vet. Sci, 90, pp.432~438 (2011)