Effects of method and duration of restraint on stress hormones and meat quality in broiler chickens with different body weights
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Objective: The study was designed to investigate the effects of restraint method, restraint duration, and body weight on stress-linked hormones (corticosterone, adrenaline, and noradrenaline), blood biochemical (namely glucose and lactate), and the meat quality in broiler chickens. Methods: A total of 120 male broiler chickens (Cobb 500) were assigned to a 2×3×2 factorial arrangement in a completely randomized design using two restraint methods (shackle and cone), three durations of restraint (10, 30, and 60 s), and two categories of live body weight (1.8±0.1 kg as lightweight and 2.8±0.1 kg as heavyweight). Results: Irrespective of the duration of restraint and body weight, the coned chickens were found to have lower plasma corticosterone (p<0.01), lactate (p<0.001), lower meat drip loss (p<0.01), cooking loss (p<0.05), and higher blood loss (p<0.05) compared with their shackled counterparts. The duration of restraint had significant effects on the meat initial pH (p<0.05), ultimate pH (p<0.05), and yellowness (p<0.01). The lightweight broilers exhibited higher (p< 0.001) blood loss and lower (p<0.05) cooking loss compared to the heavyweight broilers, regardless of the restraint method used and the duration of restraint. However, the interaction between the restraint method, duration of restraint, and body weight contributed to differences in pre-slaughter stress and meat quality. Therefore, the interaction between the restraint method and the duration of restraint affected the meat shear force, lightness (L*) and redness (a*). Conclusion: The duration of restraint and body weight undoubtedly affect stress responses and meat quality of broiler chickens. Regardless of the duration of restraint and body weight, the cone restraint resulted in notably lower stress, lower meat water loss, and higher blood loss compared to shackling. Overall, the findings of this study showed that restraint method, duration of restraint, and body weight may affect the stress response and meat quality parameters in broilers and should be considered independently or interactively in future studies.
Abstract
Objective: The study was designed to investigate the effects of restraint method, restraint duration, and body weight on stress-linked hormones (corticosterone, adrenaline, and noradrenaline), blood biochemical (namely glucose and lactate), and the meat quality in broiler chickens. Methods: A total of 120 male broiler chickens (Cobb 500) were assigned to a 2×3×2 factorial arrangement in a completely randomized design using two restraint methods (shackle and cone), three durations of restraint (10, 30, and 60 s), and two categories of live body weight (1.8±0.1 kg as lightweight and 2.8±0.1 kg as heavyweight). Results: Irrespective of the duration of restraint and body weight, the coned chickens were found to have lower plasma corticosterone (p<0.01), lactate (p<0.001), lower meat drip loss (p<0.01), cooking loss (p<0.05), and higher blood loss (p<0.05) compared with their shackled counterparts. The duration of restraint had significant effects on the meat initial pH (p<0.05), ultimate pH (p<0.05), and yellowness (p<0.01). The lightweight broilers exhibited higher (p< 0.001) blood loss and lower (p<0.05) cooking loss compared to the heavyweight broilers, regardless of the restraint method used and the duration of restraint. However, the interaction between the restraint method, duration of restraint, and body weight contributed to differences in pre-slaughter stress and meat quality. Therefore, the interaction between the restraint method and the duration of restraint affected the meat shear force, lightness (L*) and redness (a*). Conclusion: The duration of restraint and body weight undoubtedly affect stress responses and meat quality of broiler chickens. Regardless of the duration of restraint and body weight, the cone restraint resulted in notably lower stress, lower meat water loss, and higher blood loss compared to shackling. Overall, the findings of this study showed that restraint method, duration of restraint, and body weight may affect the stress response and meat quality parameters in broilers and should be considered independently or interactively in future studies.
참고문헌 (32)
- Berri C, "Variations in chicken breast meat quality : implications of struggle and muscle glycogen content at death", Br Poult Sci, 46, pp.572~579 (2005)
- Lambooij E, "The effects of captive bolt and electrical stunning, and restraining methods on broiler meat quality", Poult Sci, 78, pp.600~607 (1999)
- Yalçin S, "Interaction of transport distance and body weight on preslaughter stress and breast meat quality of broilers", Br Poult Sci, 53, pp.175~182 (2012)
- Md. Shawkat Ali, "A Review: Influences of Pre-slaughter Stress on Poultry Meat Quality", Animal Bioscience, 21(6), pp.912~916 (2008), 아세아·태평양축산학회
- Kannan G, "Shackling of broilers : effects on stress responses and breast meat quality", Br Poult Sci, 38, pp.323~332 (1997)
- Abdullah YA, "Growth performance, carcass and meat quality characteristics of different commercial crosses of broiler strains of chicken", J Poult Sci, 47, pp.13~21 (2010)
- Hillebrand SJW, "The effects of alternative electrical and mechanical stunning methods on hemorrhaging and meat quality of broiler breast and thigh muscles", Poult Sci, 75, pp.664~671 (1996)
- Kranen RW, "Hemorrhages in muscles of broiler chickens : the relationship among blood variables at various rearing temperature regimens", Poult Sci, 75, pp.570~576 (1996)
- Abdulla NR, "Physico-chemical properties of breast muscle in broiler chickens fed probiotics, antibiotics or antibiotic–probiotic mix", J Appl Anim Res, 45, pp.64~70 (2017)
- Hunt MC, "Meat color measurements", Meat Conference of the American Meat Science Assoc., pp.41~46 (1980), Purdue University
- Honikel KO, "Reference methods for the assessment of physical characteristics of meat", Meat Sci, 49, pp.447~457 (1998)
- Bouton PE, "Effect of ultimate pH upon the water-holding capacity and tenderness of mutton", J Food Sci, 36, pp.435~439 (1971)
- SAS Institute, "SAS user’s guide. Version 8.2" (1999), SAS Institute Inc
- Gao J, "Corticosterone alters meat quality by changing pre-and postslaughter muscle metabolism", Poult Sci, 87, pp.1609~1617 (2008)
- Buzzard B, "Comparison of pig restraint, sampling methods, and analysis on blood lactate concentration", Proceedings of the Kansas State University swine industry day, pp.409~414 (2012), Kansas State University
- Lyasere OS, "Elevated levels of the stress hormone, corticosterone, cause ‘pessimistic’ judgment bias in broiler chickens", Sci Rep, 7, pp.6860~ (2017)
- Nettle D, "The evolutionary origins of mood and its disorders", Curr Biol, 22, pp.712~721 (2012)
- Hermans EJ, "Dynamic adaptation of large-scale brain networks in response to acute stressors", Trends Neurosci, 37, pp.304~314 (2014)
- Gregory NG, "Duration of wing flapping in chickens shackled before slaughter", Vet Rec, 121, pp.567~569 (1987)
- Santiago HL, "Effects of strain, plane of nutrition, age at slaughter on performance and meat quality traits of broilers", Poult Sci, 84(Suppl. 1), pp.128~ (2005)
- Zhu X, "High post-mortem temperature combined with rapid glycolysis induces phosphorylase denaturation and produces pale and exudative characteristics in broiler Pectoralis major muscles", Meat Sci, 89, pp.181~188 (2011)
- Debut M, "Variation of chicken technological meat quality in relation to genotype and preslaughter stress conditions", Poult Sci, 82, pp.1829~1838 (2003)
- Debut M, "Behavioural and physiological responses of three chicken breeds to pre-slaughter shackling and acute heat stress", Br Poult Sci, 46, pp.527~535 (2005)
- Schneider BL, "Effect of holding temperature, shackling, sex, and age on broiler breast meat quality", Poult Sci, 91, pp.468~477 (2012)
- Berri CN, "Effect of selection for improved body composition on muscle and meat characteristics of broilers from experimental and commercial lines", Poult Sci, 80, pp.833~838 (2001)
- Hoving-Bolink AH, "Fiber area and capillary supply in broiler breast muscle in relation to productivity and ascites", Meat Sci, 66, pp.397~402 (2000)
- Salwani MS, "Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning", CyTA-J Food, 14, pp.375~381 (2016)
- Zhang W, "Breast meat quality of broiler chickens can be affected by managing the level of nitric oxide", Poult Sci, 92, pp.3044~3049 (2013)
- Liu R, "Contribution of nitric oxide and protein S-nitrosylation to variation in fresh meat quality", Meat Sci, 144, pp.135~148 (2018)
- Dransfield E, "Relationship between muscle growth and poultry meat quality", Poult Sci, 78, pp.743~746 (1999)
- Lyon CE, "The location of wing restraints and the incidence of residual blood in broiler breast meat", J Appl Poult Res, 3, pp.355~361 (1994)
- Huang JC, "Effects of physical restraint and electrical stunning on plasma corticosterone, postmortem metabolism, and quality of broiler breast muscle", J Anim Sci, 92, pp.5749~5756 (2014)