길항성 근권 세균이 딸기 시설재배에서 발생하는 잿빛곰팡이병의 생물학적 제어에 미치는 영향
Effects of Antagonistic Rhizobacteria on the Biological Control of ld in Greenhouse Grown Strawberry Plants
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초록
전남 담양과 장흥지역을 중심으로 딸기 시설재배지에서 발생하는 잿빛곰팡이병원균 (gray mold, Botrytis cinerea)의 대하여 길항작용이 우수한 균주를 토양으로부터 분리 동정하였다. 딸기 시설재배지에서 발생하는 잿빛곰팡이병원균에 대하여 항균작용이 우수한 균주를 10종을 1차적으로 선발하였고, 이 중에서 항균작용이 가장 우수한 SB 143을 최종적으로 선발하였다. 딸기 잿빛곰팡이병원균에 대하여 항균작용이 우수한 SB 143을 대상으로 균주의 형태적 성질, 배양적 특성 및 생리 생화학적 성질 등을 조사하여 균주의 동정을 검토한 바 Bacillus sp.와 유사한 균주로 동정되었다. 길항균으로 분리한 Bacillus sp. SB 143은 딸기 잿빛곰팡이병원균에 대하여 59.4% 정도의 높은 생장억제력을 보일 뿐만 아니라 한천배지에서 잿빛곰팡이병원균 접종 후 길항균 처리와 열처리한 균주 배양액을 처리하였을 때 각각 93.1%와 32.1%의 항균작용을 보였다.
Abstract
This study was carried out to clarify the effects of antifungal bacterial strains isolated from the greenhouse soil grown strawberry plants on the growth inhibition of plant pathogen, gray mold (Botrytis cinerea) infected in strawberry plants in Damyang and Jangheung districts. Antagonistic bacterial strains were isolated and investigated into the antagonistic activity against gray mold. Screened ten bacterial strains which strongly inhibited Botrytis cinerea were isolated from the greenhouse grown strawberry plants, and the best antifungal microorganism designated as SB 143 was finally selected. Antifungal bacterial strain SB 143 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. SB 143 showed 59.4% of antifungal activity against Botrytis cinerea. By the bacterialization of culture broth and heated filtrates of culture broth, Bacillus sp. SB 143 showed 93.1% and 32.1% of antagonistic activity against Botrytis cinerea.
참고문헌 (10)
- "Molecular basis of plant growth promotion and biocontrol by rhizobacteria.", 4(4), pp.343~350 (2001)
- "Genes and signals in the Rhizobium-legumes symbiosis", 125, pp.69~72 (2001)
- "Root nodulation and infection factors produced by rhizobial bacteria", pp.257~288 (2000)
- "Molecular basis of symbiotic promiscuity", 64, pp.180~201 (2000)
- "Viscosinamide, a new cyclic depsipeptide with surfactant and antifungal properties produced by Pseudomonas fluorescens DR54.", pp.80~90 (1999)
- "Structure, production characteristics and fungal antagonism of tensin - a new antifungal cyclic lipopeptide from Pseudomonas fluorescens strain 96.578.", pp.992~1001 (2000)
- "Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5.", (wak-thompson), pp.2166~2174 (1999)
- "Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescens Q2-87.", pp.3155~3163 (1999)
- "Regulation of production of the antifungal metabolite 2,4-diacetylphloroglucinol in Pseudomonas fluorescens F113: genetic analysis of phlF as a transcriptional repressor", pp.537~546 (2000)
- "Zwittermicin, a biosynthetic cluster", pp.403~411 (1999)