오이 탄저병원균과 잿빛곰팡이병원균에 억제 효과가 있는 방선균 선발
Selection of Antagonistic Soil Actinomycetes Against Both Colletotrichum orbiculare and Botrytis cinerea in Cucumber Plants
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The purpose of this study is to select actinomycetes with antifungal activity against Botrytis cinerea and Colletotrichum orbiculare, which are airborne pathogens in cucumber plants. In 2020, 560 species of actinomycetes were isolated from rhizome soils of various vegetables in Korea; 7 strains showing simultaneous antifungal activity against two pathogens were selected. Finally, strain S20-465 was selected through dual culture and plant assay. This strain was identified as Streptomyces sp. based on 16S rRNA analysis. The culture filtrate of strain S20-465 inhibited mycelial growth of both pathogens by more than 60%. In addition, when cucumber plants were treated with 20-fold and 40-fold diluted culture filtrates of S20-465, lesions caused by B. cinerea and C. orbiculare on cucumber leaves were significantly reduced compared to the control. This results suggest that strain S20-465 produces specific secondary metabolites with antifungal activity against both pathogens.
Abstract
The purpose of this study is to select actinomycetes with antifungal activity against Botrytis cinerea and Colletotrichum orbiculare, which are airborne pathogens in cucumber plants. In 2020, 560 species of actinomycetes were isolated from rhizome soils of various vegetables in Korea; 7 strains showing simultaneous antifungal activity against two pathogens were selected. Finally, strain S20-465 was selected through dual culture and plant assay. This strain was identified as Streptomyces sp. based on 16S rRNA analysis. The culture filtrate of strain S20-465 inhibited mycelial growth of both pathogens by more than 60%. In addition, when cucumber plants were treated with 20-fold and 40-fold diluted culture filtrates of S20-465, lesions caused by B. cinerea and C. orbiculare on cucumber leaves were significantly reduced compared to the control. This results suggest that strain S20-465 produces specific secondary metabolites with antifungal activity against both pathogens.
참고문헌 (36)
- Agrios, G. N, "Plant pathology" (2005), Elsevier Academic Press
- Avis, T. J., "Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity", Soil Biol. Biochem., 40(7), pp.1733~1740 (2008)
- Ayed, A., "Antifungal activity of volatile organic compounds from Streptomyces sp. strain S97 against Botrytis cinerea", Biocontrol. Sci. Technol., pp.1~19 (2021)
- Bérdy, J, "Bioactive microbial metabolites: a personal view", J. Antibiot., 58(1), pp.1~26 (2005)
- Choi, I. S., "Variation in phenotypic characteristics, pathogenicity and fungicides resistance of Botrytis cinerea", Kor. J. Mycol., 23(3), pp.246~256 (1995)
- Dewi, T. K., "Secondary metabolites production by actinomycetes and their antifungal activity", Kne life Sciences, 3(4), pp.256~264 (2015)
- El-Tarabily, K. A., "Performance of three encophytic actinomycetes in relation to plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the united arab emirates", Eur. J. Plant Pathol., 128(4), pp.527~539 (2010)
- Figueroa-López, A. M., "A high-throughput screening assay to identify bacterial antagonists against Fusarium verticillioides", J. Basic. Microbiol., 54(S1), pp.125~133 (2014)
- 한지희, "방선균의 식물생육촉진 효과와 식물병원균에 대한 항균활성", 농약과학회지, 16(4), pp.383~386 (2012), 한국농약과학회
- Inglin, R. C., "High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species", J. Microbiol. Methods, 114, pp.26~29 (2015)
- Iwasa, T., "Studies on validamycin, new antibiotics. II production and biological properties of validamycins A and B", J. Antibiot, XXIV, pp.107~113 (1970)
- Botrytis and Botrytis species; Taxanomy, physiology and pathogenecity, a guide to the literature, "Jarvis, W. R", Canada Department of Agriculture, ~ (1977)
- Jog, R., "Plant growth promoting potential and soil enzyme production of the most abundant Streptomyces spp. from wheat rhizosphere", J. Appl. Microbiol., 113(5), pp.1154~1164 (2012)
- 김재정, "고추 탄저병균의 포자 발아와 부착, 균사 생장에 미치는 화합물의 활성 검정법 확립 및 살균제의 효과", 농약과학회지, 7(3), pp.1~168 (2003), 한국농약과학회
- 김성훈, "국내유통농산물 중 잔류농약 모니터링 및 안전성 평가", 한국식품영양과학회지, 37(11), pp.1515~1522 (2008), 한국식품영양과학회
- Kinkel, L. L., "Streptomyces competition and coevolution in relation to plant disease suppression", Res. Microbiol., 163(8), pp.490~499 (2012)
- Development of quantitative high-throughput screening methods for identification of antifungal biocontrol strains, "Kjeldgaard, B.", ~ (2021)
- Eun Ju Ko, "Bio-Sulfur Pre-Treatment Suppresses Anthracnose on Cucumber Leaves Inoculated with Colletotrichum orbiculare", Mycobiology, 47(3), pp.308~317 (2019), 한국균학회[DOI]
- "Production and cultivated area of fruit and vegetables in South Korea"[URL]
- Kubo, Y., "Dynamics of infection-related morphogenesis and pathogenesis in Colletotrichum orbiculare", J. Gen. Plant Pathol., 79, pp.233~242 (2013)
- 이상엽, "오이 지상부의 주요 곰팡이 병해의 생물적 방제용 유용미생물의 선발", 농약과학회지, 14(4), pp.326~331 (2010), 한국농약과학회
- Lengai, G. M. W., "Biopesticides and their role in sustainable agricultural production", J. Biosci. Med., 6, pp.7~41 (2018)
- Morales, D. K., "Efficient removal of hexavalent chromium by a tolerant Streptomyces sp. affected by the toxic effect of metal exposure", J. Appl. Microbibiol., 103(6), pp.2704~2712 (2007)
- "National crop pest management system (NCPMS)"[URL]
- 박현후, "고추탄저병균 Colletotrichum scovillei 에 대한 Burkholderia cepacia KF1의 길항 활성 검정", 농업생명과학연구, 53(4), pp.45~53 (2019), 농업생명과학연구원[DOI]
- Park, D. W., "Pesticide residues and risk assessment from monitoring programs in the largest production area of leafy vegetables in South Korea: a 15-year study", Foods, 10(2), pp.425~ (2021)
- Patel, J. K., "Antibiotic producing endophytic Streptomyces spp. colonize above-ground plant parts and promote shoot growth in multiple healthy and pathogen-challenged cereal crops", Microbiol. Res., 215, pp.36~45 (2018)
- Ruanpanun, P., "Actinomyces and fungi isolated from plant-parasitic nematode infested soils: Screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production", World J. Microbiol. Biotechnol, 26(9), pp.1569~1578 (2010)
- Santoyo, G., "Mechanisms of biocontrol and plant growth promoting activity in soil bacterial species of Bacillus and Pseudomonas:A review", Biocontrol Sci. Technol., 22(9), pp.855~872 (2012)
- 심선아, "탄저병(Colletotrichum orbiculare)에 대한 박과작물의 저항성", 원예과학기술지, 31(3), pp.371~379 (2013), 한국원예학회[DOI]
- Slawecki, R. A., "Novel fungitoxicity assays for inhibition of germination-associated adhesion of Botrytis cinerea and Puccinia recondita spores", Appl. Environ. Microbiol., 68, pp.597~601 (2002)
- Takeuchi, S., "Blasticidin S, a new antibiotic", J. Antibiot., 11(1), pp.1~5 (1958)
- Umezawa, H., "A new antibiotic, Kasugamycin", J. Antibiot, 11, pp.1~5 (1965)
- Williamson, B., "Botrytis cinerea:The cause of grey mould disease", Molecular. Plant pathol., 8(5), pp.561~580 (2007)
- Yourman, L. F., "Resistance to benzimidazole and dicaboximide fungicides in greenhouse isolates of Botrytis cinerea", Plant dis, 83, pp.569~575 (1999)
- Zhang, Z. Q., "Infection assays of tomato and apple fruit by the fungal pathogen Botrytis cinerea", Bio-Protocol, 4(23), pp.e1311~ (2014)
