본문 바로가기
KERD
논문KCI 등재

Bacillus toyonensis CAB12243-2 균주를 이용한배추 무름병의 생물적 방제

Biological Control using Bacillus toyonensis Strain CAB12243-2 against Soft Rot on Chinese Cabbage

0

조회

0

다운로드

4

인용

0

참고문헌

키워드

초록

Pectobacterium carotovorum subsp. carotovorum was found to be highly virulent to various vegetables, including Chinese cabbage. The antibacterial isolate CAB12243-2 was tested in a field bioassay for suppressing soft rot disease. The nucleotide sequencing of the 16S rRNA gene identified, the CAB12243-2 strain used in this study as Bacillus toyonensis. B. toyonensis CAB12243-2 inhibited the pectate lyase process by soft rot pathogens, and used trehalose and glucose as carbon sources. In field tests, the antibacterial isolate B. toyonensis CAB12243-2 suppressed soft rot disease with 73.0% control efficacy on the spring cultivar “Norangbom” and with 68.9% efficacy on the fall cultivar “Bulam 3”. These results suggest that B. toyonensis CAB12243-2 can be used as a biological control agent for the control of soft rot diseases on vegetables.

Abstract

Pectobacterium carotovorum subsp. carotovorum was found to be highly virulent to various vegetables, including Chinese cabbage. The antibacterial isolate CAB12243-2 was tested in a field bioassay for suppressing soft rot disease. The nucleotide sequencing of the 16S rRNA gene identified, the CAB12243-2 strain used in this study as Bacillus toyonensis. B. toyonensis CAB12243-2 inhibited the pectate lyase process by soft rot pathogens, and used trehalose and glucose as carbon sources. In field tests, the antibacterial isolate B. toyonensis CAB12243-2 suppressed soft rot disease with 73.0% control efficacy on the spring cultivar “Norangbom” and with 68.9% efficacy on the fall cultivar “Bulam 3”. These results suggest that B. toyonensis CAB12243-2 can be used as a biological control agent for the control of soft rot diseases on vegetables.

참고문헌 (31)

  1. Chang, Q., "Antibiotics in agriculture and the risk to human health: how worried should we be?", Evolutionary Applications, 8, pp.240~247 (2015)[DOI]
  2. Chen, W. C., "Purification and characterization of a pectin lyase from Pythium splendens infected cucumber fruits", Bot. Bull. Acad. Sin., 39, pp.181~186 (1998)
  3. 최창현, "무름병에 감수성인 애기장대 돌연변이체 Atstp1 선발", 식물병 연구, 16(3), pp.312~315 (2010), 한국식물병리학회
  4. 정은경, "고랭지 배추 무름병 방제를 위한 우수약제 선발", 농약과학회지, 7(1), pp.5~37 (2003), 한국농약과학회
  5. Frank, J. A., "Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes", Appl. Environ. Microbiol., 74, pp.2461~2470 (2008)[DOI]
  6. Hildebrand, D. C., "Identification Schemes: Laboratory Guide for Identification of Plant Pathogenic Bacteria", pp.61~ (1988), APS Press
  7. Jimenez, G., "Description of Bacillus toyonensis sp. nov., a novel species of the Bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ANI calculations", Sys. Appl. Microbiol., 36, pp.383~391 (2013)
  8. Kantas, D., "A feed additive containing Bacillus toyonensis (Toyocerin(R)) protects against enteric pathogens in postweaning piglets", J. Appl. Biol., 118, pp.727~738 (2015)
  9. Kikumoto, T., "Ecology and biocontrol of soft rot of Chinese cabbage", J. Gen. Plant Pathol., 66, pp.275~277 (2000)[DOI]
  10. Korea Crop Protection Association (KCPA), "2017 Agrochemicals Use Guide Book", pp.1531~1532 (2017)
  11. 권영희, "배추무름병 원인균 분리 및 특성 연구", 생명과학회지, 19(8), pp.1177~1182 (2009), 한국생명과학회
  12. Kyeremeh, A. G., "Biological control of soft rot of chinese cabbage using single and mixed treatments of bacteriocin-producing avirulent mutants of Erwinia carotovora subsp. carotovora", J. Gen. Plant Pathol., 66, pp.264~268 (2000)[DOI]
  13. Lee, Y. K., "Occurrence of soft rot on Chinese cabbage and characters of related phytopathogenic bacteria", Proceeding of Korean Society of Pesticide Science, pp.176~ (2015)
  14. McKenna, M., "Antibiotic resistance: The last resort", Nature, 499, pp.394~396 (2013)
  15. The Numerical Statement of Agriculture, Food and Rural Affairs, "Ministry of Agriculture, Food and Rural Affairs (MAFRA)", 308~309 (2016)
  16. Panda, P., "Pectobacterium atrosepticum and Pectobacterium carotovorum harbor distinct, independently acquired integrative and conjugative elements encoding coronafacic acid that enhance virulence on potato stems", Frontiers in Microbiol, 7, pp.1~13 (2016)
  17. Pandin, C., "Antimicrobial peptide resistance genes in the plant pathogen Dickeya dadantii", Appl. Environ. Microbiol., 82, pp.6423~6430 (2016)[DOI]
  18. Rural Development Administration (RDA), "Damage assessment for nine main crops by degree of outbreak in disease and insect", pp.114~127 (2016)
  19. Santos, F. D. S., "Bacillus toyonensis improves immune response in the mice vaccinated with recombinant antigen of bovine herpesvirus type 5", Beneficial Microbes (2017)[DOI]
  20. 아누파마 슈레스타, "Biological Control of Soft Rot on Chinese Cabbage Using Beneficial Bacterial Agents in Greenhouse and Field", 농약과학회지, 13(4), pp.325~331 (2009), 한국농약과학회
  21. Statistics Korea, "Crop Production in 2016", pp.74~81 (2017)
  22. Tallur, P. N., "Characterization of antibiotic resistant and enzyme producing bacterial strains isolated from the Arabian Sea", 3 Biotech (2016)[DOI]
  23. Tamura, K., "MEGA6: Molecular Evolutionary Genetics Analysis version 6.0", Mol. Biol. Evol., 30, pp.2725~2729 (2013)[DOI]
  24. The Korean Society of Plant Pathology (KSPP), "List of Plant Disease in Korea", pp.106~ (2009), The Korean Society of Plant Pathology
  25. Togashi, J., "Overwintering of Erwinia carotovora subsp. carotovora in diseased tissues in soil and its role as inoculum for soft rot of Chinese cabbage (Brassica campestris, Pekinensis group)", J. Gen. Plant Pathol., 67, pp.45~50 (2001)[DOI]
  26. Toth, I. K., "Rotting softy and stealthily", Current Opinion in Plant Biol., 8, pp.424~429 (2005)[DOI]
  27. Toth, I. K., "Soft ot erwiniae: from genes to genomes", Mol. Plant Pathol., 4, pp.17~30 (2003)[DOI]
  28. Tournas, V. H., "Spoilage of vegetable crops by bacteria and fungi and related health hazard", Critical Rev. in Microbiol., 31, pp.33~44 (2005)
  29. Tsuda, K., "Biological control of bacterial soft rot in Chinese cabbage by Lactobacillus plantarum strain BY under field conditions", Biological Control, 100, pp.63~69 (2016)[DOI]
  30. Zhao, Y., "Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action", World J. Microbiol. Biotech., 29, pp.411~420 (2013)[DOI]
  31. Zucker, M., "Inducible pectate lyase synthesis and phytopathogenicity of Pseudomonas fluorescens", Canadian J. Microbiol., 17, pp.1313~1318 (1971)

관련 논문AI추천

고추 탄저병 방제제 Burkholderia lata CAB13001선발 및 포장방제 효과권미경, 김병련, 박인희, 서경원, 한광섭, 함수상한국유기농업학회 · 한국유기농업학회지2018 · 제26권 제4호Assessment of the resistance of bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum KACC 21701 in Kimchi cabbage genetic resources고호철, 김빛샘, 김성훈, 노나영, 성정숙, 이윤정, 이재은, 이주희, 이호선, 한범수, 허온숙, 황예진, Awraris Derbie Assefa, Parthiban Subramanian한국환경생물학회 · 환경생물2022 · 제40권 제4호Isolation and Characterization of Burkholderia cepacia strain YJK2, Antagonistic Microorganism ofPaprika Pathogens김형무, 양수정, 주호종한국유기농업학회 · 한국유기농업학회지2015 · 제23권 제1호취나물 점무늬병을 억제하는 Paenibacillus polymyxa JE201의 생물학적 특성김다연, 박병용, 안성호, 이상엽, 한지희한국유기농업학회 · 한국유기농업학회지2021 · 제29권 제2호Bacillus sp. JJ2-01과 마늘 오일 혼합처리에 의한고추 흰비단병 억제 효과문혜정, 상미경, 송재경, 안성호, 주호종한국유기농업학회 · 한국유기농업학회지2022 · 제30권 제3호길항성 근권 세균이 딸기 시설재배에서 발생하는 잿빛곰팡이병의 생물학적 제어에 미치는 영향양승렬, 조자용, 조정일한국유기농업학회 · 한국유기농업학회지2005 · 제13권 제2호

이 간행물의 다른 논문 (10건)