Harnessing Rimocidins-Producing Streptomyces sp. JCK-6116 as a Sustainable Fungicide for Biocontrol of Cucumber Soil-Borne Diseases
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Fusarium oxysporum f. sp. cucumerinum, the causal agent of cucumber fusarium wilt, along with Rhizoctonia solani AG-4 and Pythium ultimum—that causes cucumber damping-off—are soil-borne fungal and Oomycetes pathogens responsible for significant economic losses in agriculture. In this study, the culture filtrate of Streptomyces sp. JCK-6116, isolated from soil, exhibited strong inhibitory activity against the mycelial growth of multiple phytopathogenic fungi in a 96-well microtiter plate assay. In the dual culture assay, JCK-6116 inhibited the growth of 20 species of plant pathogenic fungi and Oomycetes, suggesting a wide antifungal spectrum. Three active compounds— rimocidin A, B, and C—were isolated from JCK-6116 and identified. These rimocidins exhibited antifungal effects against fungi by binding to ergosterol in the fungal membrane. However, none of the compounds exhibited anti-oomycete activity against the tested Oomycetes strains. Among the three compounds, rimocidin A demonstrated the strongest antifungal activity with minimum inhibitory concentration values ranging from 1.25–10 μg/ml. Furthermore, the culture broth of JCK6116, at 10-fold dilution, effectively suppressed fusarium wilt and the two damping-off diseases in cucumber. Its butanol extract was also effective against the two fungal diseases but showed no activity against P. ultimum damping-off disease. These findings indicate that the culture broth contains metabolites with anti-oomycete activity. This study demonstrates that Streptomyces sp. JCK-6116 has significant potential as a biological control agent for managing soil-borne diseases caused by fungi and Oomycetes.
Abstract
Fusarium oxysporum f. sp. cucumerinum, the causal agent of cucumber fusarium wilt, along with Rhizoctonia solani AG-4 and Pythium ultimum—that causes cucumber damping-off—are soil-borne fungal and Oomycetes pathogens responsible for significant economic losses in agriculture. In this study, the culture filtrate of Streptomyces sp. JCK-6116, isolated from soil, exhibited strong inhibitory activity against the mycelial growth of multiple phytopathogenic fungi in a 96-well microtiter plate assay. In the dual culture assay, JCK-6116 inhibited the growth of 20 species of plant pathogenic fungi and Oomycetes, suggesting a wide antifungal spectrum. Three active compounds— rimocidin A, B, and C—were isolated from JCK-6116 and identified. These rimocidins exhibited antifungal effects against fungi by binding to ergosterol in the fungal membrane. However, none of the compounds exhibited anti-oomycete activity against the tested Oomycetes strains. Among the three compounds, rimocidin A demonstrated the strongest antifungal activity with minimum inhibitory concentration values ranging from 1.25–10 μg/ml. Furthermore, the culture broth of JCK6116, at 10-fold dilution, effectively suppressed fusarium wilt and the two damping-off diseases in cucumber. Its butanol extract was also effective against the two fungal diseases but showed no activity against P. ultimum damping-off disease. These findings indicate that the culture broth contains metabolites with anti-oomycete activity. This study demonstrates that Streptomyces sp. JCK-6116 has significant potential as a biological control agent for managing soil-borne diseases caused by fungi and Oomycetes.