갈퀴덩굴 추출물에 의한 RAW 264.7 세포에서의 항염 효과
Anti-inflammatory Effect of Galium aparine Extract in RAW 264.7 Cells
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목적: 본 연구는 lipopolysaccharide (LPS)에 의해 염증이 유도된 RAW 264.7 세포에서 갈퀴덩굴(Galium aparine , GA) 추출물의염증억제 효과를 확인하기 위하여 수행되었다. 방법: GA 추출물이 RAW 264.7 세포에서 항염 효과를 나타내는지 확인하기 위하여1,1-diphenyl-2-picrylhydrazyl (DPPH) radical 소거 활성, GA 추출물의 세포 생존율 측정, 사이토카인의 발현양상, nitric oxide (NO)와 prostaglandin E2 (PGE2)의 생성 정도를 확인하였다. 결과: GA 추출물이 interleukin 1α (IL1α ), interleukin 1β (IL1β), interleukin 6 (IL6 ), tumor necrosis factor α (TNFα ) 유전자 발현을 감소시킴을 확인할 수 있었다. 또한 NO 생성을 감소시키고PGE2 단백질 생성을 감소시킴을 통해 GA 추출물의 항염 효과를 확인할 수 있었다. 결론: 본 연구를 통해 GA 추출물의 항염 효과를 확인하였고, GA 추출물이 항염 및 아토피를 타겟으로 하는 화장품 원료로서의 가능성을 제시하였다. 앞으로 GA 추출물이 피부미용, 의약품 산업 등에도 응용될 수 있을 것으로 여겨지며 그 가능성을 확인하기 위해 GA 추출물이 염증세포에 미치는 기전 연구가 필요할 것으로 보인다.
Abstract
Purpose: This study was performed to identify the skin anti-inflammatory effect of Galium aparine (GA) extract on RAW 264.7 cells. Methods: To investigate antiinflammatory effects of GA on RAW 264.7 cells, we measured 1,1-Diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity, cell viability, mRNA expressions, nitric oxide (NO), and prostaglandin E2 (PGE2) production. Results: In this study, we elucidated anti-inflammatory effects of GA extract on interleukin 1α (IL1α ), interleukin 1β (IL1β ) interleukin 6 (IL6 ), and tumor necrosis factor α (TNFα ) mRNA expression and NO production. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that the GA extract decreased mRNA expression level of IL1α , IL1β, IL6, and TNFα and detection of NO and PGE2 also revealed that GA induces anti-inflammatory effects. Conclusion: Therefore, we successfully identified the anti-inflammatory effect of GA extract, and this result showed that the GA extract can be a potent cosmetic ingredient for anti-inflammatory and atopic dermatitis. Based on this, we anticipate further studies on the GA extract to determine a mechanism for developing not only cosmetics but also healthcare food or medicines.
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