브라질린의 RAW264.7 세포에서의 항염효과
Anti-inflammatory Effect of Brazilin in RAW264.7 Cells
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목적: 본 연구는 브라질린에 의한 RAW264.7 세포의 항염 효과를 확인하기 위하여 수행되었다. 방법: 브라질린이 RAW264.7세포에 항염효과를 나타내는지 확인하기 위하여, CCK-8 실험법을 통한 세포생존률 측정, 실시간 유전자 중합효소 연쇄반응을 통한 싸이토카인의 유전자 발현양상, 효소결합면역흡착검사법을 통한 PGE 2 와 산화질소의 생성 정도를 확인하였다. 결과: RAW264.7 세포에 브라질린을 처리하여 세포독성을 확인한 결과, 1 μg/mL 이하의 농도로 처리시 독성을 나타내지 않음을 확인할 수 있었다. RAW264.7 세포는 LPS 자극에 의해 세포질 내 소과립이 증가되나 브라질린을 처리하면 대조군과 유사하게 세포질 내 소과립이 감소하는 경향을 보였다. 본 연구를 통해 브라질린이 인터류킨1α (interleukin 1α, IL1α), 인터류킨1β (interleukin 1β, IL1β), 인터류킨 6 (interleukin 6, IL6), tumor necrosis factor α (TNFα) 유전자의 발현을 감소시킴을 확인할 수 있었다. 또한 산화질소의 생성을 감소시키고 PGE 2 의 생성을 감소시킴을 통해 브라질린의 항염효과를 확인할 수 있었다. 결론: 본 연구를 통해 브라질린의 항염효과를 확인하였고, 브라질린이 항염 및 아토피를 타겟으로 하는 화장품 원료로서의 가능성을 제시하였다. 브라질린이 피부미용, 화장품, 건강식품 등의 산업 등에도 응용될 수 있을 것으로 여겨지며 그 가능을 확인하기 위해 브라질린이 염증세포에 미치는 기전연구가 필요할 것으로 보인다.
Abstract
Purpose: This study aimed to determine the skin anti-inflammatory effect of brazilin on RAW264.7 cells. Methods: To determine anti-inflammatory effects of brazilin on RAW264.7 cells, we measured cell viability, gene expressions, and prostaglandin E 2 (PGE 2 )/nitric oxide (NO) production. Results: Cells were treated with brazilin to confirm cytotoxicity; no toxicity was observed when cells were treated at a concentration of ≤1 μg/mL. In RAW264.7 cells, the number of intracellular granule were increased after lipopolysaccharide (LPS) treatment; however, brazilin treatment decreased intracellular granules similar to that in the control group. In this research, we elucidated the anti-inflammatory effects of brazilin on the mRNA expression of interleukin 1 alpha (IL1α), interleukin 1 beta (IL1β), interleukin 6 (IL6), and tumor necrosis factor alpha (TNFα) and PGE 2 /NO production. Real-time reverse transcription polymerase chain reaction (real-time RT-PCR) showed that brazilin administration increased the mRNA levels of IL1α /IL1β /IL6 /TNFα genes, and analysis of PGE 2 /NO also revealed that brazilin induces anti-inflammatory effects. Conclusion: We identified the anti-inflammatory effect of brazilin, and found that brazilin can be a potent cosmetic ingredient for anti-inflammatory agent for atopic dermatitis. Based on this result, we believe that brazilin has the potential to be used in not only cosmetics, but also medicine or healthcare food.
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