모델 담즙산 나노입자 기반의 새로운 피부전달체
A Novel Transdermal Delivery System based on a Bile AcidConjugated Nanoparticle Model for Cosmetics
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목적: 모델 담즙산-PhS1P/PS 나노입자를 함유한 화장품 제형화 및 생체 내 효능을 평가하기 위하여 소수성인 담즙산의 주성분인 sodium deoxycholate 및 cholesterol과 lecithin을 기반으로 한 phytosphingosine-1-phosphate (PhS1P) 및 phytosterol (PS)을 효과적인 피부전달체로 개발하고자 한다. 방법: PhS1P 및 PS는 초음파 처리 방법에 의해 모델 담즙산-PhS1P/PS 나노입자에 봉입 되었다. 모델 담즙산-PhS1P/PS 나노입자의 피부침투율을 평가하기 위해 franz diffusion cell 실험을 수행하였고 원료에 대한 적용 가능성을 알아보았다. 모델 담즙산-PhS1P/PS 나노입자는 입도 분석기, SEM 및 HPLC 실험으로 특성을 분석하였고, 피부에 대한 효과를 확인하기 위하여 최종 시제품으로 임상평가를 진행하였으며, Cutometer 및 Dermascan-C를 사용하여 피부의 탄력, 피부의 두께 및 진피 치밀도를 측정하여 그 개선 효과를 확인하였다. 결과: 모델 담즙산-PhS1P/PS 나노입자를 85%의 높은 수율로 제조하였으며 평균직경이 약 120.3 nm로 확인되어 PhS1P 및 PS의 피부세포 침투효과가 대조군 보다 더 우수함을 입증하였다. 이 사실로부터 모델 담즙산-PhS1P/PS 나노입자가 피부세포로 침투되어 PhS1P 및 PS로 나뉘어 방출되고 진피세포로 쉽게 흡수된다는 것을 알 수 있었고, 피부에 대한 나노입자의 안전성 평가와 피부의 탄력, 피부의 두께 및 진피 치밀도 개선효과와 이상 증상이 없음을 관찰하였다. 따라서 모델 담즙산-PhS1P/PS 나노입자는 훌륭한 피부전달체 및 화장품 소재임을 확인하였다. 결론: 화장품 제형에 모델 담즙산-PhS1P/PS 나노입자를 사용함으로써 유효성분의 피부흡수 및 전달이 용이하였으며, 향후 화장품에 적용할 수 있는 중요한 소재로 기대할 수 있다.
Abstract
Purpose: We assessed the in vivo efficacy of a cosmetic formulation comprising a transdermal delivery system of model bile acid nanoparticles after the development of a phytosphingosine-1-phosphate (PhS1P)/phytosterol (PS) carrier based on chemically modified lecithin with sodium deoxycholate, using cholesterol as a hydrophobic component. Methods: PhS1P/PS was loaded into a bile acidconjugated nanoparticle (PhS1P/PS-MBA nanoparticle) model via sonication. Franz diffusion cell experiment was performed to evaluate the skin penetration efficacy of the prepared nanoparticles and to comprehend the feasibility of applying the raw material. The nanoparticles were confirmed using a particle size analyzer, scanning electron microscope, and high-performance liquid chromatography system. In order to understand the effectiveness of the nanoparticles on the skin, we performed an in vivo test in human volunteers using the finished product. The elasticity, thickness, and dermal density of the applied skin were examined by using Cutometer (Courage+Khazaka electronic GmbH, Germany). Results: We confirmed through our tests that the nanoparticles could be successfully prepared with 85% yield and were approximately 120.3 nm in diameter. The product showed more efficient skin permeation than the control, thereby suggesting an easier uptake within the cells and a concomitant fast release of cleaved PhS1P/PS into the human skin. Safety assessment, elasticity, thickness, and dermal density of the nanoparticles on the human skin were examined to test the possible applicability of the nanoparticles to cosmetics. No adverse symptoms were observed. Conclusion: It is therefore recommended that cosmetic products should use PhS1P/PS-MBA nanoparticles in their formulation for easier absorbability into the skin. Our experiment confirms that this new material may have valuable influences on the human skin.
참고문헌 (26)
- Bouic PJ, "The role of phytosterols and phytosterolins in immune modulation: a review of the past 10 years", Current Opinion in Clinical Nutrition and Metabolic Care, 4, pp.471~475 (2001)
- Cho HJ, "Quantitative study of stratum corneum ceramides contents in patients with sensitive skin", The Journal of Dermatology, 39, pp.295~300 (2012)
- 정지연, "화장품의 경피 흡수에 대한 최신 연구 동향", 아시안뷰티화장품학술지, 12(5), pp.597~605 (2014), 한국피부과학연구원
- Chung N, "Sphingolipids signal heat stress-induced ubiquitindependent proteolysis", Journal of Biological Chemistry, 275, pp.17229~17232 (2000)
- Coderch L, "Ceramides and skin function", American Journal of Clinical Dermatology, 4, pp.107~129 (2003)
- Han NR, "The β-sitosterol attenuates atopic dermatitis-like skin lesions through downregulation of TSLP", Experimental Biology and Medicine, 239, pp.454~464 (2014)
- Hjelm RP, "Form and structure of self-assembling particles in monoolein-bile salt mixtures", The Journal of Physical Chemistry, 99, pp.16395~16406 (1995)
- Hofmann AF, "Detergent properties of bile salts:correlation with physiological function", Annual Review of Medicine, 18, pp.333~376 (1967)
- Hofmann AF, "Bile acid solubility and precipitation in vitro and in vivo : the role of conjugation, pH, and Ca2+ions", Journal of Lipid Research, 33, pp.617~626 (1992)
- Kim DM, "Composition for external skin application for preventing pigmentation and promoting skin regeneration and a manufacturing method thereof", Korea Patent 10-2018-0170393, 2018.12.27, ~ (2018)
- Kim HJ, "The functional cosmetics and process used biles and jahageo-the Chinese medicine named the navel cord", Korea Patent 10-2003-0020270, 2003.03.31, ~ (2003)
- Lee JP, "Phytosphigosine-1-phosphate represses the hydrogen peroxide-induced activation of c-Jun-Nterminal kinase in human dermal fibroblasts through the phosphatidylinositol 3-kinase", Akt pathway, 2012, pp.304, 673~678
- 이미나, "5 nm급 Nano Gold Essence 화장품의 유효성 평가", 아시안뷰티화장품학술지, 14(3), pp.259~266 (2016), 한국피부과학연구원[DOI]
- Loizou S, "β-Sitosterol exhibits anti-inflammatory activity in human aortic endothelial cells", Molecular Nutrition Food Research, 54, pp.551~558 (2010)
- Mi LZ, "Structural basis for bile-acid binding and activation of the nuclear receptor FXR", Molecular Cell, 11, pp.1093~1100 (2003)
- Nurunnabi Md, "Design and strategies for bile acid mediated therapy and imaging", Royal Society of Chemistry Advances, 6, pp.73986~74002 (2016)
- Pata MO, "Plant sphingolipids : decoding the enigma of the Sphinx", The New Phytologist, 185, pp.611~630 (2010)
- Pyne S, "Sphingosine 1-phosphate signalling in mammalian cells", The Biochemical Journal, 349, pp.385~402 (2000)
- Roda A, "The influence of bile salts structure on self-association in aqueous solution", The Journal of Biological Chemistry, 258, pp.6362~6370 (1983)
- Setchell KDR, "The bile acids:chemistry, physiology and metabolism-Volume 4:methods and applications", pp.122~145 (1988), Springer US
- Jeong Hyun Shin, "Sphingosine 1-Phosphate Triggers Apoptotic Signal for B16 Melanoma Cells via ERK and Caspase Activation", Journal of Korean Medical Science, 22(2), pp.298~304 (2007), 대한의학회
- Stamp D, "Bile acids:toxicology and bioactivity", pp.1~13 (2008), Royal Society of Chemistry
- Yun JS, "Fast releasing micellar nanoparticle and a cosmetic composition comprising it", Korea Patent 10-2017-0143761, 2017.10.31, ~ (2017)
- Yun JS, "Multifuntional sun formulation composition", Korea Patent 10-2018-0103402, 2018a.08.31, ~ (2018)
- Yun JS, "17 materials of oriental medicine fermentated cosmetic composition and method for preparing thereof", Korea Patent 10-2018-0113465, 2018b.09.21, ~ (2018)
- Yun JS, "Fast releasing micellar nanoparticle and a cosmetic composition comprising it", International Patent PCT, 2018