LPS로 자극한 RAW264.7세포에서 브로콜리 잎 헥산 분획물의 항염증 효과
Anti-inflammatory Effect of Broccoli Leaf Hexane Fraction in LPS-stimulated RAW264.7 Cells
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초록
본 연구에서는 브로콜리 잎 헥산 분획물의 항염 효과를 평가하여 기능성 식품 및 화장품 소재로의 적용 가능성을 확인하였다. LPS-자극된 RAW264.7 세포에서 전염증성 사이토카인의 생성, iNOS와 COX-2의 발현, MAPK (ERK, JNK, p38) 및 브로콜리 잎 헥산 분획을 사용한 NF-κB의 인산화를 분석하였다. 브로콜리 잎 헥산 분획은 TNF-α, IL-4, IL-6, IL-1β 등의 전염증성 사이토카인의 분비와 iNOS와 COX-2의 발현을 억제했습니다. 또한, 브로콜리 잎 헥산 분획물은 MAPK와 NF- κB의 인산화를 감소시켰다. 따라서 브로콜리 잎 헥산 분획물은 식품 및 화장품에서 천연 항염증소재로 적용 가능성이 있는 것으로 판단된다. 향후 항염증 기전 및 주요 생리활성 물질의 규명에대한 연구가 필요할 것으로 생각된다.
Abstract
In this study, we tested the anti-inflammatory effects of broccoli leaf hexane fraction to confirm the applicability as a functional material in food and cosmetics. This sample was extracted using 70% ethanol from Broccoli leaf and then fractionated with hexane. The production of pro-inflammatory cytokines (TNF-α, IL-4, IL-6, IL-1β), protein expression of iNOS and COX-2, phosphorylation of MAPKs (ERK, JNK, p38) and NF-κB with broccoli leaf hexane fraction were assayed on LPS-stimulated RAW264.7 cells. The broccoli leaf hexane fraction inhibited the secretion of pro-inflammatory cytokines and protein expression of iNOS and COX-2. Also, the broccoli leaf hexane fraction reduced the phosphorylation of MAPKs and NF-κB. Therefore, it is considered that th broccoli leaf hexane fraction has the potential to be used as a natural anti-inflammatory material in food and cosmetics. In the future, it is considered necessary to study the anti-inflammatory mechanism and identification of major bioactive substances.
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