라말린이 함유된 제품의 항산화 효과 및 피부노화 완화 효과
Effects of In Vitro Antioxidant and In Vivo Anti-aging Improvement of Finished Cosmetic Products Containing Ramalin
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Although the studies of ingredient with antioxidant effect have been performing, in vitro and in vivo study of product containing antioxidant was not sufficient relatively. Potential antioxidants are very unstable due to auto-oxidation despite of their stabilized form exposed to air or sunlight. Especially, cosmetics were made through the process of high temperature, homogenization and mixture of a lot of ingredients. So, we cannot assure whether antioxidants keep the anti-oxidative effi cacy on the fi nished product without any confi rming test. In this study, we investigated antioxidant effect through DPPH and ORAC method and human volunteer test of three types of products with Actosome Ramalin as a stabilized form of Ramalin reported potent antioxidant previously. IC50 value of fi nished products, Essence, Ample and Cream, was 6.7%, 41.62% and 3.2%respectively. ORAC value showed 1034.52μM TE/g, 953.41μM TE/g and 1023.49μM TE/g. In in vivo human test of Essence, skin hydration, texture, brightness and lifting were statistically improved after 3 weeks and 6weeks compared to before using product. In the case of Ample, skin elasticity of R5 parameter on the side of eye was statistically improved after 2 weeks and 4 weeks compared to before using product. Cream statistically improved skin texture and skin tone after 2 weeks and 4 weeks. Any problem in the skin was not revealed in human volunteer test. From these results, we suggest that we can claim the skin anti-oxidative effect of product in the demonstration of anti-oxidative effect of finished product. And products formulated only antioxidant as active ingredient were improved and alleviated phenomena of facial skin aging in human volunteer test. Therefore, anti-oxidative effi cacy demonstration of products containing anti-oxidant will be more practical and realistic approach to measure anti-oxidative level than raw material test.
Abstract
Although the studies of ingredient with antioxidant effect have been performing, in vitro and in vivo study of product containing antioxidant was not sufficient relatively. Potential antioxidants are very unstable due to auto-oxidation despite of their stabilized form exposed to air or sunlight. Especially, cosmetics were made through the process of high temperature, homogenization and mixture of a lot of ingredients. So, we cannot assure whether antioxidants keep the anti-oxidative effi cacy on the fi nished product without any confi rming test. In this study, we investigated antioxidant effect through DPPH and ORAC method and human volunteer test of three types of products with Actosome Ramalin as a stabilized form of Ramalin reported potent antioxidant previously. IC50 value of fi nished products, Essence, Ample and Cream, was 6.7%, 41.62% and 3.2%respectively. ORAC value showed 1034.52μM TE/g, 953.41μM TE/g and 1023.49μM TE/g. In in vivo human test of Essence, skin hydration, texture, brightness and lifting were statistically improved after 3 weeks and 6weeks compared to before using product. In the case of Ample, skin elasticity of R5 parameter on the side of eye was statistically improved after 2 weeks and 4 weeks compared to before using product. Cream statistically improved skin texture and skin tone after 2 weeks and 4 weeks. Any problem in the skin was not revealed in human volunteer test. From these results, we suggest that we can claim the skin anti-oxidative effect of product in the demonstration of anti-oxidative effect of finished product. And products formulated only antioxidant as active ingredient were improved and alleviated phenomena of facial skin aging in human volunteer test. Therefore, anti-oxidative effi cacy demonstration of products containing anti-oxidant will be more practical and realistic approach to measure anti-oxidative level than raw material test.
참고문헌 (27)
- 강신정, "흑삼을 함유한 한방소재 화장품의 주름개선 효과", 한국산학기술학회논문지, 11(9), pp.3325~3329 (2010), 한국산학기술학회
- 김남수, "미세주름 측정을 위한 비접촉식 영상측정기술의 발전", 대한화장품학회지, 31, pp.253~257 (2005)
- 김성환, "ORAC Assay에 의한 인삼의 항산화 활성 연구", 동아시아식생활학회지, 17(3), pp.393~401 (2007), 동아시아식생활학회
- 김수현, "더덕(Codonopsis lanceolata) 추출물의 in vitro 및 in vivo 항산화 효과", 한국식품영양과학회지, 39(2), pp.193~202 (2010), 한국식품영양과학회
- 김승훈1, 박성규2, "玄蔘, 白茯？, 金銀花, 馬齒？, 白果葉을 함유하는 한방화장품이 인체 피부의 보습 및 청열 효능에 미치는 영향", 대한본초학회지, 22(2), pp.45~50 (2007), 대한본초학회
- 박신영, "3D 측정 기기를 이용한 얼굴의 부피 정량법 연구", 대한화장품학회지, 34(4), pp.333~338 (2008), 대한화장품학회
- 박창민, "3-O-cetyl-L-ascorbic acid의 주름 개선 효과", 대한화장품학회지, 34(4), pp.303~309 (2008), 대한화장품학회
- 양웅석, "사매추출물을 함유하는 화장품의 주름 개선 효과", 대한화장품학회지, 36(4), pp.281~288 (2010), 대한화장품학회
- 양윤정, "국내 자생 자원식물들의 항산화 활성 검색", 한국자원식물학회지, 24(1), pp.1~9 (2011), 한국자원식물학회
- 임태진, "만병초(Rhododendron brachycarpum) 추출물의 항산화 효과", 한국자원식물학회지, 24(4), pp.456~460 (2011), 한국자원식물학회
- 정춘복, "투명감 측정을 통한 제형의 미백 효능 평가와 투명감에 관여하는 요소들에 대한 분석", 대한화장품학회지, 36(4), pp.253~258 (2010), 대한화장품학회
- 조가영, "다크서클(Dark Circles under Eyes)과 담음(痰飮)의 연관성에관한 연구", 대한한의진단학회지, 1, pp.49~60 (2009)
- 최정섭, "96-well plate를 이용한 DPPH free radical 소거활성 측정과 그 응용", 농약과학회지, 7(2), pp.3~99 (2003), 한국농약과학회
- Ahn SY, "Correlation between a CutometerⓇ and quantitative evaluation using Moire topography in age-related skin elasticity", Skin Res. Technol, 13, pp.280~284 (2007)
- Akira K, "A new approach to the evaluation of whitening effect of a cosmetic using computer analysis of video-captured image", J. Dermatol. Sci, 29, pp.10~18 (2002)
- Ana Z, "ORAC and TEAC assay comparison to measure the antioxidant capacity of food products", Food Chem, 114, pp.310~316 (2009)
- Babita P, "Antibacterial Activities of Ramalin, Usnic Acid and its Three Derivatives Isolated from the Antarctic Lichen Ramalina terebrata", Z. Naturforsch, 65, pp.34~38 (2010)
- 한세종, "Ramalin, a novel nontoxic antioxidant compound from the Antarctic lichen Ramalina terebrata", PHYTOMEDICINE, 18(14), pp.1285~1290, ELSEVIER GMBH
- Carol SY, "Combined infrared light and bipolar radiofrequency for skin tightening in Asians", Lasers Surg . Med, 39, pp.471~475 (2007)
- Eric VK, "Reproducibility of repeated measurements on healthy skin with Minolta Chromameter CR-300", Skin Res. Technol, 7, pp.56~59 (2001)
- Giuseppe GB, "Experimental and numerical study on the mechanical behavior of the superficial layers of the face", Skin Res . Technol, 17, pp.434~444 (2011)
- Islam MR, "Antioxidant Activity of the Ethanol Extract of Manilkara zapota Leaf", J. Sci. Res, 4, pp.193~202 (2012)
- Naoko S, "Development of a new evaluation method for cheek sagging using a Moire 3D analysis system", Skin Res. Technol, 14, pp.287~292 (2008)
- Nicola PYC, "Fractional ablative carbon dioxide laser resurfacing for skin rejuvenation and acne scars in Asians", Lasers Surg. Med, 42, pp.615~623 (2010)
- Nils K, "Agerelated changes in skin mechanical properties: a quantitative evaluation of 120 female subjects, Skin Res", Technol, 17, pp.141~148 (2011)
- Türkan K, "Antioxidant properties of different extracts of black mulberry (Morus nigra L.)", Turk. J. Biol, 35, pp.103~110 (2011)
- Heinrich U, "Multicentre comparison of skin hydration in terms of physical-, physiological- and product-dependent parameters by the capacitive method (Corneometer CM 825)", Int.J. Cosmet. Sci, 25, pp.24~53 (2003)