커큐민이 지방유래 줄기세포 계대배양에 미치는 영향
Effect of Curcumin on the Adipose Tissue-derived Stem Cell Subculture
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Adipose-derived stem cells (ASCs), which have characteristics of self-renewal and multipotency, areresponsible for the tissue regeneration of the damaged tissue. Replicative senescence is the cause of anirreversible arrest of cell proliferation and loss cell function. Reactive oxygen species (ROS) are producedas a normal byproduct of aerobic life but the accumulation of the oxidative damage due to ROS induces thefundamental changes in cell aging process. In this study, the anti-aging effects of curcumin was studied inthe long-term culture of ASCs by determining the expression of SA-β-galactosidase. Cell protection effect ofcurcumin was observed in UVB irradiated ASCs. Antioxidant, or anti-aging effect of curcumin by scavengingof intra-cellular ROS or enhancing the expression of antioxidant enzymes was also confirmed. Thesedata suggest that curcumin may be effective in preventing the rise of oxidative stress during adipocytedifferentiation through mechanisms that involves upregulation of endogenous antioxidant responses.
Abstract
Adipose-derived stem cells (ASCs), which have characteristics of self-renewal and multipotency, areresponsible for the tissue regeneration of the damaged tissue. Replicative senescence is the cause of anirreversible arrest of cell proliferation and loss cell function. Reactive oxygen species (ROS) are producedas a normal byproduct of aerobic life but the accumulation of the oxidative damage due to ROS induces thefundamental changes in cell aging process. In this study, the anti-aging effects of curcumin was studied inthe long-term culture of ASCs by determining the expression of SA-β-galactosidase. Cell protection effect ofcurcumin was observed in UVB irradiated ASCs. Antioxidant, or anti-aging effect of curcumin by scavengingof intra-cellular ROS or enhancing the expression of antioxidant enzymes was also confirmed. Thesedata suggest that curcumin may be effective in preventing the rise of oxidative stress during adipocytedifferentiation through mechanisms that involves upregulation of endogenous antioxidant responses.
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