Rapid Isolation of Adipose Tissue-Derived Stem Cells by the Storage of Lipoaspirates
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Purpose: This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs). Materials and Methods: Aliquots (10 g) of the lipoaspirates were stored at 4°C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed. Results: When the lipoaspirates were stored at 4°C, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1×1014 fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells. Conclusion: ASCs isolated from lipoaspirates and stored for 24 hours at 4°C have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4°C for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their stemness for a long time, like ASCs isolated from fresh lipoaspirates.
Abstract
Purpose: This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs). Materials and Methods: Aliquots (10 g) of the lipoaspirates were stored at 4°C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed. Results: When the lipoaspirates were stored at 4°C, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1×1014 fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells. Conclusion: ASCs isolated from lipoaspirates and stored for 24 hours at 4°C have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4°C for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their stemness for a long time, like ASCs isolated from fresh lipoaspirates.
참고문헌 (37)
- Colter DC, "Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow", Proc Natl Acad Sci U S A, 97, pp.3213~3218 (2000)
- Sekiya I, "Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality", Stem Cells, 20, pp.530~541 (2002)
- Haynesworth SE, "Characterization of cells with osteogenic potential from human marrow", Bone, 13, pp.81~88 (1992)
- Pittenger MF, "Multilineage potential of adult human mesenchymal stem cells", Science, 284, pp.143~147 (1999)
- Prockop DJ, "Marrow stromal cells as stem cells for nonhematopoietic tissues", Science, 276, pp.71~74 (1997)
- Friedenstein AJ, "Precursor cells of mechanocytes", Int Rev Cytol, 47, pp.327~359 (1976)
- Friedenstein AJ, "Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers", Cell Tissue Kinet, 20, pp.263~272 (1987)
- Friedenstein AJ, "Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues", Transplantation, 6, pp.230~247 (1968)
- Owen M, "Stromal stem cells: marrow-derived osteogenic precursors", Ciba Found Symp, 136, pp.42~60 (1988)
- Campagnoli C, "Identification of mesenchymal stem", progenitor cells in human first-trimester fetal blood, liver, and bone marrow, 2001, pp.98, 2396~2402
- De Ugarte DA, "Comparison of multi-lineage cells from human adipose tissue and bone marrow", Cells Tissues Organs, 174, pp.101~109 (2003)
- Erices A, "Mesenchymal progenitor cells in human umbilical cord blood", Br J Haematol, 109, pp.235~242 (2000)
- Jiang Y, "Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain", Exp Hematol, 30, pp.896~904 (2002)
- Kuznetsov SA, "Circulating skeletal stem cells", J Cell Biol, 153, pp.1133~1140 (2001)
- Lee MW, "Isolation of mesenchymal stem cells from cryopreserved human umbilical cord blood", INTERNATIONAL JOURNAL OF HEMATOLOGY, 81, pp.126~130 (2005), SPRINGER TOKYO
- Noort WA, "Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34(+) cells in NOD", SCID mice, 2002, pp.30, 870~878
- Young HE, "Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors", Anat Rec, 264, pp.51~62 (2001)
- Zvaifler NJ, "Mesenchymal precursor cells in the blood of normal individuals", Arthritis Res, 2, pp.477~488 (2000)
- Rebelatto CK, "Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue", Exp Biol Med (Maywood), 233, pp.901~913 (2008)
- Sakaguchi Y, "Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source", Arthritis Rheum, 52, pp.2521~2529 (2005)
- Zuk PA, "Multilineage cells from human adipose tissue: implications for cell-based therapies", Tissue Eng, 7, pp.211~228 (2001)
- Strem BM, "Multipotential differentiation of adipose tissue-derived stem cells", Keio J Med, 54, pp.132~141 (2005)
- Hoffmann J, "Angiogenic effects despite limited cell survival of bone marrow-derived mesenchymal stem cells under ischemia", Thorac Cardiovasc Surg, 58, pp.136~142 (2010)
- Mylotte LA, "Metabolic flexibility permits mesenchymal stem cell survival in an ischemic environment", Stem Cells, 26, pp.1325~1336 (2008)
- Matsumoto D, "Influences of preservation at various temperatures on liposuction aspirates", Plast Reconstr Surg, 120, pp.1510~1517 (2007)
- Keck M, "Local anesthetics have a major impact on viability of preadipocytes and their differentiation into adipocytes", Plast Reconstr Surg, 126, pp.1500~1505 (2010)
- Preece A, "A manual for histologic technicians" (1972), Little, Brown
- Hu Y, "Isolation and identification of mesenchymal stem cells from human fetal pancreas", J Lab Clin Med, 141, pp.342~349 (2003)
- Martin JY, "Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces", Clin Oral Implants Res, 7, pp.27~37 (1996)
- Woodbury D, "Adult rat and human bone marrow stromal cells differentiate into neurons", J Neurosci Res, 61, pp.364~370 (2000)
- Santa María L, "Signals from damaged but not undamaged skeletal muscle induce myogenic differentiation of rat bone-marrow-derived mesenchymal stem cells", Exp Cell Res, 300, pp.418~426 (2004)
- Xu W, "Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro", Exp Biol Med (Maywood), 229, pp.623~631 (2004)
- Eom YW, "Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 408(1), pp.167~173 (2011), ACADEMIC PRESS INC ELSEVIER SCIENCE
- Yoo KH, "Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues", CELLULAR IMMUNOLOGY, 259(2), pp.150~156 (2009), ACADEMIC PRESS INC ELSEVIER SCIENCE
- Gronthos S, "Surface protein characterization of human adipose tissue-derived stromal cells", J Cell Physiol, 189, pp.54~63 (2001)
- Zuk PA, "Human adipose tissue is a source of multipotent stem cells", Mol Biol Cell, 13, pp.4279~4295 (2002)
- Puissant B, "Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells", Br J Haematol, 129, pp.118~129 (2005)