알리인(Alliin)의 인간진피섬유아세포 보호효과 및 Collagen 발현 증가, MMP1 발현 억제 효과에 관한 연구
Effects of Alliin on Cellular Protection, Up-regulation of Collagen and Down-regulation of MMP1 in Human Dermal Fibroblasts
0
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
The attention to aging has been reflected in various aspects. In the cosmetics industry, the development ofanti-aging products has been in a steady progress. Anti-inflammatory and antioxidant research has beenconducted using garlic extract, but relevant research and development have been insufficient due to thedifficulties to use garlic as a cosmetic ingredient due to its characteristics such as the distinctive scent ofgarlic. Thus, based on the need for development of an ingredient to complement the weaknesses, alliinwas selected as the research subject as it is heat-stable and also its activity is maintained in the dry state. The present study aims to examine the effect of alliin in inhibition of aging by giving normal human dermalfibroblast (NHDF) H2O2-oxidative stress. In order to test the efficacy of alliin, 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide thiazolyl blue assay, enzyme-linkes immunosorbent assay, and quantitativereal-time polymerase chain reaction were performed. It was demonstrated that treating H2O2-treated NHDFwith alliin was effective in restoring damaged cells as the treatment increase the survival rate of the cell. Itwas also demonstrated that alliin treatment given to the NHDF treated with H2O2-oxidative stress facilitatedthe generation of procollagen type I and inhibited the generation of MMP1 protein. In addition, the alliintreatment was found to facilitate the gene expression of the COL1A1 and inhibit the gene expression ofMMP1 . Taken together, the effect of alliin in improving aged skin was demonstrated in the cell efficacy test,and alliin is expected to have a value as an excellent anti-aging ingredient for functional cosmetics.
Abstract
The attention to aging has been reflected in various aspects. In the cosmetics industry, the development ofanti-aging products has been in a steady progress. Anti-inflammatory and antioxidant research has beenconducted using garlic extract, but relevant research and development have been insufficient due to thedifficulties to use garlic as a cosmetic ingredient due to its characteristics such as the distinctive scent ofgarlic. Thus, based on the need for development of an ingredient to complement the weaknesses, alliinwas selected as the research subject as it is heat-stable and also its activity is maintained in the dry state. The present study aims to examine the effect of alliin in inhibition of aging by giving normal human dermalfibroblast (NHDF) H2O2-oxidative stress. In order to test the efficacy of alliin, 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide thiazolyl blue assay, enzyme-linkes immunosorbent assay, and quantitativereal-time polymerase chain reaction were performed. It was demonstrated that treating H2O2-treated NHDFwith alliin was effective in restoring damaged cells as the treatment increase the survival rate of the cell. Itwas also demonstrated that alliin treatment given to the NHDF treated with H2O2-oxidative stress facilitatedthe generation of procollagen type I and inhibited the generation of MMP1 protein. In addition, the alliintreatment was found to facilitate the gene expression of the COL1A1 and inhibit the gene expression ofMMP1 . Taken together, the effect of alliin in improving aged skin was demonstrated in the cell efficacy test,and alliin is expected to have a value as an excellent anti-aging ingredient for functional cosmetics.
참고문헌 (33)
- 김나영, "자외선을 조사한 인간 섬유아세포에서 황금추출물과 황금주요플라보 노이 성분이 MMP-1 발현에 미치는 영향", 아시안뷰티화장품저널, 11(4), pp.649~654 (2013), 한국피부임상과학연구소
- 김선희, "흑마늘 화장품의 주름개선 및 미백에 대한 피부미용 효과", 한국미용학회지, 15(3), pp.1041~1050 (2009), 한국미용학회
- 김영주, "Centella asiatica 추출물의 인간 진피 섬유아세포에서 H₂O₂로 유도된 세포노화에 대한 조절작용", 박사, Cosmetic Biology, pp.2011
- 배인순, "황백 열수추출물의 피부노화 억제 효과 및 화장품 소재 활용에 관한 연구", 박사, 공중보건학, pp.2008
- 강원식, "녹차카테킨이 지질과 산화 및 superoxide dismutase에 미치는영향", 한국식품위생안전성학회지, 16, pp.41~47 (2001)
- 김나미, "홍삼성분이 섬유아세포의 콜라겐 생합성과 MMP-I 활성에 미치는 영향", Journal of Ginseng Research, 31(2), pp.86~92 (2007), 고려인삼학회
- 김지은, "인체 피부 섬유아세포에서 열자극에 의해 유도되는 type Ⅰ procollagen, MMP-1과 TIMP-1 발현에 대한 녹차추출물(epigallocatechin-3-gallate)의 효과", 석사, 유전공학, pp.2004
- 박애영, "마늘추출물의 항균성과 두피건강에 미치는 영향", 석사, 피부비만관리학, pp.2011
- 손명수, "홍삼에서 추출한 오일의 항산화 및 보습활성", 대한피부미용학회지, 11(3), pp.489~494 (2013), 대한피부미용학회
- 이민호, "진피섬유모세포에서 대복피추출물의 세포외기질 합성 촉진 효과", 한국산학기술학회논문지, 14(4), pp.1857~1862 (2013), 한국산학기술학회[DOI]
- 윤혜영, "마늘추출물로 제조한 화장품의 여드름 억제효과에 관한 연구", 석사, 미용향장학, pp.2005
- 전지민, "유산균 발효에 의한 인삼열매 추출물의 항산화 및 항노화 효과", 대한화장품학회지, 37(1), pp.75~81 (2011), 대한화장품학회
- 최현경, "인체 피부 세포를 이용한 라비지아 추출물의 항노화 효능에 관한 연구", 석사, 화학공학, pp.2009
- 한상미, "산화적 스트레스에 대한 국내산 로얄제리의 신경세포 보호 효과", Journal of Apiculture, 26(2), pp.113~117 (2011), 한국양봉학회
- Ariga T, "Antithrombotic and anticancer ettects of garlic-derived sulfur compounds: a review", Biofactors, 26, pp.93~103 (2006)
- Banerjee SK, "Effect of garlic on cardiovascular disorders", Nutr. J, 19, pp.1~4 (2002)
- Castanet J, "Pigmentary changes is aged and photoaged skin", Arch. Dermatol, 133, pp.1296~1299 (1997)
- Chow HH, "Modulation of human glutathione S-transferases by polyphenone intervention", intervention. Cancer Epidemiol Biomarkers Prev, 16, pp.1662~1666 (2007)
- Chung JH., "Photoaging in Asians. Photodermatol. Photoimmunol", Photomed, 19, pp.109~121 (2003)
- Fisher GJ, "Molecular basis of sun-induced premature skin ageing and retinoid antagonism", Nature, 379, pp.335~339 (1996)
- Hachiya A, "Mechanistic effects of longterm ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts", Am. J. Pathol, 174, pp.401~413 (2009)
- Higuchi R, "Simultaneous amplification and detection of specific DNA sequences", Biotechnology (N Y), 10, pp.413~417 (1992)
- Iberl B, "Quantitative determination of allicin and alliin from garlic by HPLC", Planta. Med, 56, pp.320~326 (1990)
- Iciek M, "Biological properties of garlic and garlic-derived organosulfur compounds. Environ", Mol. Mutagen. Rev, 50, pp.247~265 (2009)
- 김인덕, "한방원료의 초임계 추출을 이용한 항노화 및 주름개선 효과", KSBB Journal, 23(6), pp.529~534 (2008), 한국생물공학회
- Kovacs D, "Keratinocytes growth factor down-regulates intracellular ROS production induced by UVB", J. Dermatol. Sci, 54, pp.107~113 (2009)
- Kourounakis PN, "Effect on active oxygen species of alliin and Allium sativum (garlic) powder", Commun. Chem. Pathol. Pharmacol, 74, pp.249~252 (1991)
- Kwon SK, "Organosulfur compounds from Allium sativum and physiological activities", J. Appl. Pharmacol, 11, pp.8~32 (2003)
- Lee GY, "Literature review on the effect of human skin following garlic extraction", J. Beau. Tricho, 3, pp.6~12 (2007)
- Mosmann T., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J. Immunol. Methods, 65, pp.1~55-63, (1983)
- Uitto J, "Extracellular matrix of the skin: 50 years of progress", J. Invest. Dermatol, 92, pp.61S~77S (1989)
- Waller JM, "Age and skin structure and function, aquantitative approach (Ⅱ):protein, glycosamonoglycan, water, and lipid content and structure", Skin. Res. Technol, 12, pp.145~154 (2006)
- Yokoyama Y1, "Histologic study of collagen and stem cells after radiofrequency treatment for aging skin", Dermatol. Surg, 40, pp.390~397 (2014)