멜라닌 생합성 저해를 통한 지모뿌리추출물에서의 새로운 천연 미백소재의 개발
New Development of Natural Depigmentation Agent from Anemarrhena asphodeloides Root Extracts by Inhibition of Melanin Biosynthesis
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
목적: 본 연구는 지모뿌리추출물에 의한 미백효과를 확인하기 위한 것이다. 방법: 지모뿌리추출물에 의한 tyrosinase 활성 억제 및 B16F10 melanoma 세포를 이용하여 멜라닌 생합성을 측정하였으며 지모뿌리추출물 크림을 제조하여 임상연구도 함께 수행하였다. 결과: Tyrosinase 활성 및 α-melanocyte stimulating hormone (α-MSH)에 의하여 유도되는 멜라닌 생합성이 지모뿌리추출물에 의하여 농도 의존적으로 저해되는 것을 확인하였다. 특히, 25 μg/mL의 농도에서 지모뿌리추출물(28.4%)은 잘 알려진 멜라닌 생합성 저해제인 알부틴(24.7%)보다 더 효과적으로 멜라닌 생합성을 저해하였다. 임상연구에서는, 자외선으로 시험자의 피부에 색소침착을 유도한 후 지모뿌리추출물이 2% 함유된 크림 제형을 사용하도록 한 후 8 주 동안 피부 미백 효과를 조사하였다. 시험 결과에 의하면 과색소침착 정도, 멜라닌 지수, 그리고 피부 밝기가 유의적으로 개선되었다. 결론: 지모뿌리추출물은 피부 미백을 위한 기능성 화장품 소재로서의 높은 가능성을 기대하게 한다
Abstract
Purpose: This study aimed to confim the depigmentation effects from Anemarrhena asphodeloides root extracts (AARE). Methods: We assessed tyrosinase inhibition activity of AARE and investigated the biosynthesis of melanin in B16F10 melanoma cells and subjected to clinical trials with AARE creams. Results: Tyrosinase activity and melanin biosynthesis induced by α-melanocyte stimulating hormone (α-MSH) were inhibited by AARE in a concentration-dependent manner. In particular, at 25 μg/mL concentration, melanin biosynthesis was more effectively inhibited by AARE (28.4%) than arbutin (24.7%; a well-known melanin synthesis inhibitor). In clinical trials, we investigated the changes in human skin brightness values over eight weeks using a cream formulation that contained 2% AARE after inducing pigmentation via ultraviolet irradiation. We showed that the hyperpigmentation degree, melanin index, and skin brightness were signifiantly improved. Conclusion: AARE is expected to become widely available as a functional cosmetic material for skin whitening.
참고문헌 (25)
- Briganti S, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell & Melanoma Research, 16, pp.101~110 (2003)
- Chakraborty AK, "Effect of arbutin on melanogenic proteins in human melanocytes", Pigment Cell & Melanoma Research, 11, pp.206~212 (1998)
- Esposito D, "Steroidal glycosides from the bulbs of Easter lily (Lilium longiflorum Thunb.) promote dermal fibroblast migration in vitro", Journal of Ethnopharmacology, 148, pp.433~440 (2013)
- Fitzpatrick TB, "Soleil et peau", Journal de Médecine Esthétique, 2, pp.33~34 (1975)
- Gilchrest BA, "DNA photodamage stimulates melanogenesis and other photoprotective responses", Journal of Investigative Dermatology Symposium Proceedings, 4, pp.35~40 (1999)
- Hill HZ, "Melanin: a two edged sword?", Pigment Cell & Melanoma Research, 10, pp.158~161 (1997)
- Iida Y, "In vitro synergism between nyasol, an active compound isolated from Anemarrhena asphodeloides , and azole agents against Candida albicans", Planta Medica, 66, pp.435~438 (2000)
- Imokawa G, "The role of endothelin-1 in epidermal hyperpigmentation and signaling mechanisms of mitogenesis and melanogenesis", Pigment Cell & Melanoma Research, 10, pp.218~228 (1997)
- 강민철, "제주도 자생 노루참나물 (Pimpinella komarovii) 추출물의 항산화 효과 및 멜라닌 억제 효과", KSBB Journal, 23(1), pp.77~82 (2008), 한국생물공학회
- Ministry of Food and Drug Safety, "for evaluating the functional cosmetics (I)" (2003)
- Miura T, "Antidiabetic activity of the rhizoma of Anemarrhena asphodeloides and active components, mangiferin and its glucoside", Biological and Pharmaceutical Bulletin, 24, pp.1009~1011 (2001)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65, pp.55~63 (1983)
- Park HJ, "Isolation and antioomycete activity of nyasol from Anemarrhena asphodeloides rhizomes", Phytochemistry, 64, pp.997~1001 (2003)
- 박정옥, "추출 방법에 따른 비파엽 추출물의 미백 및 항염활성에 관한 연구", 대한화장품학회지, 41(2), pp.151~157 (2015), 사단법인 대한화장품학회[DOI]
- 박윤정, "지모의 뿌리줄기로부터 분리된 nyasol의 미용효과", Journal of Applied Biological Chemistry, 58(1), pp.31~38 (2015), 한국응용생명화학회
- 유민정, "SK-MEL-2에서 상백피 추출물의 Tyrosinase 활성 억제 및 Melanin 생성 억제 효과", 아시안뷰티화장품학술지, 9(4), pp.1~12 (2011), 한국피부과학연구원
- Solano F, "Hypopigmenting agents : an updated review on biological, chemical and clinical aspects", Pigment Cell & Melanoma Research, 19, pp.550~571 (2006)
- Son KH, "Isolation and quantitative determination of Anemarsaponin B from the rhizomes of Anemarrhena asphodeloides Bunge", Korean Journal of Pharmacognosy, 9, pp.131~135 (1999)
- Sy LK, "Timosaponin A-Ⅲinduces autophagy preceding mitochondria-mediated apoptosis in HeLa cancer cells", Cancer Research, 68, pp.10229~10237 (2008)
- Tang Z, "Anemarrhena asphodeloides non-steroidal saponin components alter the pharmacokinetic profile of its steroidal saponins in rat", Molecules, 20, pp.11777~11792 (2015)
- Taylor SC, "Skin of color: biology, structure, function, and implications for dermatologic disease", Journal of the American Academy of Dermatology, 46, pp.S41~S62 (2002)
- Thody AJ, "Does alpha-MSH have a role in regulating skin pigmentation in humans?", Pigment Cell & Melanoma Research, 11, pp.265~274 (1998)
- Lam Do Phuong Uyen, "Mechanism of Skin Pigmentation", Biotechnology and Bioprocess Engineering, 13(4), pp.383~395 (2008), 한국생물공학회
- Wang GJ, "Effect of timosaponin A-III, from Anemarrhenae asphodeloides Bunge (Liliaceae), on calcium mobilization in vascular endothelial and smooth muscle cells and on vascular tension", Life Sciences, 71, pp.1081~1090 (2002)
- Yagi A, "Inhibition of mushroomtyrosinase by Aloe extract", Planta Medica, 53, pp.515~517 (1987)