지방유래 3차원(3D) 줄기세포 조건화 배지가 함유된 크림이 피부탄력에 미치는 영향
Effects of Cream Containing Three-Dimensional Human Adipose- Derived Mesenchymal Stem Cell Conditioned Medium Secreting Growth Factors on Skin Elasticity
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초록
목적: 본 연구에서는 3차원 지방조직유래 중간엽줄기세포 조건화 배지(three-dimensional human adipose tissue derived mesenchymal stem cell conditioned medium, 3D ADMSC-CM)를 함유한 크림이 피부탄력에 미치는 효능에 관한 연구를 진행하고자 한다. 방법: 3D ADMSC-CM의 세포 증식능력, EGF와 VEGF의 발현량을 확인한다. 3D ADMSC-CM이 함유된 크림의 콜라겐(collagen1) 발현량을 검증하며, 리프팅, 피부탄력, 피부탄성복원력 등 임상적 효능 평가를 진행한다. 결과: 인간 진피유래 섬유아 세포의 형태적 변화가 관찰되지 않았으며, 농도 의존적으로 세포의 생존율이 증가하는 것으로 나타났다. EGF의 분비량은 2.692 pg/mL, VEGF는 27.89 pg/mL을 분비하였다. 3D ADMSC-CM 크림의 콜라겐(collagen 1)이 발현량은 183%로 증가하는 것을 확인 할 수 있었다. 3D ADMSC-CM 크림 사용에 의한 임상적 효능 평가에서 리프팅 효과를 측정한 결과 적용 2주 후와 4주 후에 유의미한 효과를 보였다. 피부탄력 및 피부탄성복원력을 변수 별 측정값을 분석한 결과 적용 2주 후와 적용 4주 후에 모두 개선 효과가 있는 것으로 나타났다. 결론: 본 연구에서 인체 구조와 비슷한 환경에서 배양된 3D ADMSC-CM이 함유된 크림을 통해 피부탄력 증진에 매우 효과적이며, 3D ADMSC-CM은 코스메슈티컬 시장을 선도할 것으로 기대된다
Abstract
Purpose: In this study, the effect on skin elasticity of a cream containing threedimensional human adipose tissue derived mesenchymal stem cell conditioned medium (3D ADMSC-CM) was examined. Methods: The clinical efficacy of the cream was evaluated by examining the skin for skin lifting, elasticity, and resilience after examining the cell proliferative capacity, expression levels of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) in 3D ADMSC-CM, and collagen expression in skin cells due to the 3D ADMSC-CM cream. Results: Skin cell viability was well maintained, with no cytotoxicity from the 3D ADMSC-CM. The amount of EGF and VEGF contained in the 3D ADMSC-CM was 2.692 pg/mL and 27.89 pg/mL, respectively. In addition, skin cells treated with the 3D ADMSC-CM cream experienced a collagen expression increase of 183%. In clinical evaluation of the 3D ADMSC-CM cream, skin lifting and skin elasticity improvement showed significant effects at both two and four weeks. After the 3D ADMSC-CM cream was used, the improved skin elasticity and resilience was analyzed by variables, and an improvement was observed in both after two and four weeks of use. Conclusion: A cream containing 3D ADMSC-CM derived from a 3D culture environment, similar to that of an in vivo environment, is highly effective in enhancing skin elasticity, and 3D ADMSC-CM is expected to lead the field of cosmeceuticals.
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