쌍발이모자반(Sargassum patens) 추출물의 항산화 및 항염효과
Antioxidant and Anti-inflammatory activity of Sargassum patens extract
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본 연구에서는 쌍발이모자반 추출물의 항산화능 및 항염능을 확인하였다. 항산화능 실험에는 폴리페놀 농도 측정, 플라보노이드 농도 측정, DPPH 실험, ABTS 실험, NO 실험, FRAP 실험을 실시하였다. 폴리페놀의 경우 18.99±0.69 mg/g으로 나타났다. 플라보노이드의 경우 11.89±1.16 mg/g으로 나타났다. DPPH 실험에서는 19.78 mg ascorbic acid / g extract의 항산화능을 나타내었으며, ABTS 실험에서는 63.64 mg ascorbic acid / g extract의 항산화능을 나타내었으며, NO 실험에서는 7.966 mg ascorbic acid / g extract의 항산화능을 나타내었다. FRAP에서는 쌍발이모자반 추출물의 1 mg이 ascorbic acid 2.089 μg의 환원력을 보였다. 한편 세포실험에서는 세포 독성과 LPS로 유도된 염증에 대한 항염능을 알아보았다. 세포독성의 경우 모든 농도에서 80%이상의 세포 생존률을 보였으며, NO 생성 억제능의 경우 100 μg/mL 농도에서 30.64±0.23%의 염증 억제능을 보여 쌍발이모자반 추출물이 항염능을 가진 화장품 원료로서 사용가능함을 보였다.
Abstract
In this study, the antioxidant and anti-inflammatory properties of Sargassum patens extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 18.99±0.69 mg/g was shown. Flavonoids showed 11.89±1.16 mg/g. The DPPH experiment showed an antioxidant function of 19.78 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 63.64 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 7.966 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 2.089 μg of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Sargassum patens extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 30.64±0.23% at a concentration of 100 μg/mL. These results indicate that Sargassum patens extract is available as an anti-inflammatory cosmetic material.
참고문헌 (25)
- 이주희, "해조류 청각(Codium fragile) 에탄올 추출물의 항산화에 관한 연구", 문화기술의 융합, 5(4), pp.467~472 (2019), 국제문화기술진흥원
- T. Suganya, "Macroalgae and microalgae as a potential source for commercial applications along with biofuels production : a biorefinery approach", Renewable and Sustainable Energy Reviews, 55, pp.909~941 (2016)[DOI]
- 정주희, "해양생물 추출물의 대장암세포주에 대한 항암 작용 검색", 한국식품위생안전성학회지, 32(1), pp.70~74 (2017), 한국식품위생안전성학회[DOI]
- L. O’Sullivan, "Prebiotics from marine macroalgae for human and animal health applications", Marine drugs, 8(7), pp.2038~2064 (2010)[DOI]
- C. Deville, "Study on the effects of laminarin, a polysaccharide from seaweed, on gut characteristics", Journal of the Science of Food and Agriculture, 87(9), pp.1717~1725 (2007)[DOI]
- J. I. Choi, "Structural feature and antioxidant activity of low molecular weight laminarin degraded by gamma irradiation", Food chemistry, 129(2), pp.520~523 (2011)[DOI]
- A. M. Gamal-Eldeen, "In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium", Food and Chemical Toxicology, 47(6), pp.1378~1384 (2009)[DOI]
- S. Ananthi, "In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata(Marine Brown Alga)", Food and chemical toxicology, 48(1), pp.187~192 (2010)[DOI]
- T. T. Dang, "Comparison of chemical profile and antioxidant properties of the brown algae", International Journal of Food Science &Technology, 53(1), pp.174~181 (2018)[DOI]
- A. Ratz-Lyko, "Methods for evaluation of cosmetic antioxidant capacity", Skin Research and Technology, 18(4), pp.421~430 (2012)[DOI]
- M. Schieber, "ROS function in redox signaling and oxidative stress", Current biology, 24(10), pp.R453~R462 (2014)[DOI]
- H. Masaki, "Role of antioxidants in the skin : anti-aging effects", Journal of dermatological science, 58(2), pp.85~90 (2010)[DOI]
- J. W. Shin, "Molecular mechanisms of dermal aging and antiaging approaches", International journal of molecular sciences, 20(9), pp.2126~ (2019)[DOI]
- I. G. Munteanu, "Analytical Methods Used in Determining Antioxidant Activity: A Review", International Journal of Molecular Sciences, 22(7), pp.3380~ (2021)[DOI]
- A. Pękal, "Evaluation of aluminium complexation reaction for flavonoid content assay", Food Analytical Methods, 7(9), pp.1776~1782 (2014)[DOI]
- M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, 181(4617), pp.1199~1200 (1958)
- R. Re, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free radical biology and medicine, 26(9-10), pp.1231~1237 (1999)[DOI]
- Ganesh Chandra Jagetia, "The Evaluation of Nitric Oxide Scavenging Activity of Certain Indian Medicinal Plants In Vitro: A Preliminary Study", Journal of Medicinal Food, 7(3), pp.343~348 (2004), 한국식품영양과학회
- 손현정, "해조류 추출물의 In Vitro 항치매 활성", 한국식품영양과학회지, 45(7), pp.966~972 (2016), 한국식품영양과학회
- 곽충실, "한국산 5종 해조류의 항산화효과와 총 폴리페놀 함량과의 관련성", 한국식품영양과학회지, 34(8), pp.1143~1150 (2005), 한국식품영양과학회
- B. Alexander, "A simple and accurate mathematical method for calculation of the EC50", Journal of pharmacological and toxicological methods, 41(2-3), pp.55~58 (1999)[DOI]
- Z. Demirel, "Antimicrobial and antioxidant activity of brown algae from the Aegean Sea", Journal of the Serbian Chemical Society, 74(6), pp.619~628 (2009)[DOI]
- Z. Demirel, "Antimicrobial and antioxidant activity of brown algae from the Aegean Sea", Journal of the Serbian Chemical Society, 74(6), pp.619~628 (2009)[DOI]
- E. Apostolova, "Immunomodulatory and Anti-Inflammatory Effects of Fucoidan : A Review", Polymers, 12(10), pp.2338~ (2020)[DOI]
- A. Cumashi, "A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds", Glycobiology, 17(5), pp.541~552 (2007)[DOI]