타트체리(Prnus cerasus) 추출물의 피부상재균에 대한 항균 활성
Antimicrobial Activities against Skin Flora from Tart-cherry Extracts (Prnus cerasus)
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목적: 본 연구에서는 타트체리 열수 추출물 (TCW)과 에탄올 추출물 (TCE)을 대상으로 피부상재균에 대한 항균 활성과 항산화 활성을 측정하였으며, RAW264.7 macrophage cell에 대하여 세포 생존율과 항염증 효능을 조사하여 기능성 화장품 소재로서 특성을 분석하였다. 방법: DPPH method에 의한 항산화 활성과 피부상재균의 항균 활성은 DISC 확산법에 의한 clear zone의 크기와 MIC 측정방법을 통하여 gram positive bacteria와 gram negative bacteria에 대한 활성을 확인하였고, 항생제 내성 균주인 MRSA strain에 대한 활성도 측정하였다. LPS로 염증반응을 유도한 RAW264.7 macrophage cell에 대한 세포 독성과 NO 활성을 측정하여 항염증 반응을 규명하였다. 결과: 타트체리 추출물의 항산화 활성은 시료의 농도 500 μg/mL에서 TCE (85.6%), TCW (88.4%)의 높은 활성을 나타내었다. 피부상재균에 대한 항균 활성은 Cutibacterium acnes (18 mm), Staphylococcus epidermis (4 mm) 및 Staphylococcus aureus (16 mm)로 clear zone의 크기를 확인할 수 있었고, Candida albicans에 대한 항진균 활성은 나타내지 않았다. RAW264.7 macrophage cell에 대한 세포 독성은 250 μg/mL 이하의 농도에서 90% 이상의 생존율을 보였으며, NO를 측정한 결과 농도의존적으로 NO 생성을 저해하는 것으로 조사되었다. 결론: 타트체리 추출물은 높은 항산화 활성과 항염증 효능을 확인할 수 있었다. 피부상재균인 Staphylococcus aureus와 Staphylococcus epidermis에 대한 활성과 더불어 여드름 유발 균주인 Cutibacterium acnes에 높은 항균 활성을 확인함으로 화장품 소재 및 바이오 제품 원료로써 충분한 가능성을 시사하는 바이다.
Abstract
Purpose: In this study, antibacterial and anti-oxidant activities of Tart-cherry hydrothermal extracts (TCW) and ethanol extracts (TCE) against dermatological bacteria were measured, and the cell viability and anti-inflammatory effects were investigated in RAW264.7 macrophages to determine their characteristics for use as functional cosmetic materials. Methods: Anti-oxidant activity was determined using the DPPH method. The antibacterial activity against dermatophilosis was confirmed by the size of the clear zone formed by DISK diffusion and MIC measurement. The activity against an antibiotic-resistant MRSA strain was also measured. Anti-inflammatory response was identified by measuring cytotoxicity and NO activity in RAW264.7 macrophages, in which inflammatory reactions were induced with LPS. Results: Anti-oxidant activity of the TCE was 85.6%, and of TCW was 88.4% at 500 μg/mL. The size of the clear zone was confirmed using Cutibacterium acnes (18 mm), Staphylococcus epidermis (S. epidermis) (4 mm), and Staphylococcus aureus (S. aureus) (16 mm). No antifungal activity against Candida albicans was observed. RAW264.7 macrophages showed a survival rate of >90% at <250 μg/mL of extract. Additionally, a concentration-dependent inhibition of NO production was observed. Conclusion: Tart-cherry extracts possessed high anti-oxidant activity and anti-inflammatory efficacy. In addition to the antibacterial activity against the skin-residing bacteria, S. aureus and S. epidermis, high activity against the acne-causing strain C. acnes suggests a sufficient potential of Tart-cherry extracts as a raw material for the preparation of cosmetics and bioproducts.
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