화장품 소재로서 Hawaiian Sandalwood (Santalum paniculatum) Essential Oil의 항산화 및 항노화 효능 분석
Evaluation of Antioxidant and Anti-aging Effects of Hawaiian Sandalwood (Santalum paniculatum) Essential Oil as a Cosmetic Ingredient
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목적: 본 연구는 하와이안 샌달우드(Santalum paniculantum) 에센셜 오일(HSEO)의 항산화 및 항노화 특성을 평가하고, 상기 결과를 기반으로 기능성 화장품 소재로의 개발 가능성을 탐색하고자 하였다. 방법: HSEO의 항산화 성분은 총 폴리페놀 함량 및 총 플라보노이드 함량 분석법을 통해 정량화되었다. HSEO의 항산화 효능은 ABTS 및 DPPH radical 소거 활성 시험을 통해 평가되었고, HSEO의 항노화 효과는 UVB가 조사된 CCD-986sk 세포를 이용한 in vitro 시험을 통해 수행되었다. 결과: HSEO는 1.703±0.050 mg GAE/g의 폴리페놀과 0.291±0.017 mg QE/g의 플라보노이드를 포함하고 있는 것으로 확인되었다. 이러한 천연 항산화 성분들로 인해, 1 mg/mL 농도의 HSEO는 92.2±0.6%의 DPPH radical 소거 활성과 99.8±0.1%의 ABTS radical 소거 활성을 나타내는 등 뛰어난 항산화 효능을 보였다. 또한 UVB (20 mJ/cm2) 조사로 인해 CCD-986sk 세포 내 콜라겐 함량이 38.42±1.17%로 감소하였으나, HSEO를 3.125, 6.25, 12.5 ng/mL 농도로 처리한 경우 각각 58.32±2.36%, 77.50±0.60%, 98.77±1.38%로 콜라겐 함량이 상당히 회복됨을 나타냈다. 이러한 결과는 세포 내 UVB에 의한 콜라겐 감소를 HSEO가 개선시킬 수 있음을 보여준다. 결론: 따라서 본 연구 결과를 통해 HSEO는 상당한 항산화 및 항노화 효과를 보유하고 있음을 보여주며, 이를 통해 안전하면서도 효과적인 기능성 화장품 원료로의 활용 가능성을 제시한다.
Abstract
Purpose: This study aimed to assess the anti-oxidant and anti-aging properties of Hawaiian Sandalwood (Santalum paniculatum) essential oil (HSEO) to investigate its potential as a functional cosmetic ingredient. Methods: The antioxidant components of HSEO were quantified with the total phenolic content and total flavonoid content assays. Its antioxidant efficacy was further evaluated through the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. An in vitro experiment was conducted on CCD-986sk cells, focusing on its ability to inhibit collagen degradation, to assess its anti-aging effect. Results: HSEO contained 1.703±0.050 mg of GAE/g of polyphenols and 0.291±0.017 mg of QE/g of flavonoids. These abundant natural anti-oxidant compounds endowed HSEO with notable radical scavenging activities, as evidenced by its 92.2%±0.6% DPPH and 99.8%±0.1% ABTS radical scavenging activities at a 1 mg/mL concentration. Additionally, ultraviolet B (20 mJ/cm2) irradiation reduced collagen levels in CCD-986sk cells to 38.42%±1.17%. However, HSEO cotreatment at 3.125, 6.25, and 12.5 ng/mL concentrations restored collagen levels to 58.32% ±2.36%, 77.50%±0.60%, and 98.77%±1.38%, respectively, exhibiting its significant anti-aging potential. Conclusion: These results indicate that HSEO possesses significant anti-oxidant and anti-aging properties, supporting its potential as an effective functional cosmetic ingredient.
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