작약 뿌리 추출물의 Pentagalloylglucose 분석법 확립 및 유효성 검증
Validation of the HPLC Method for the Standardization of Pentagalloylglucose in Paeonia lactiflora Root Extract as a Functional Ingredient
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초록
목적: 본 연구는 HPLC를 이용하여 작약 뿌리 추출물의 pentagalloylglucose을 지표물질로 설정하고 분석법을 확립하여 유효성 검증을 하였다. 방법: Pentagalloylglucose 분석을 위해 C18컬럼을 이용하여 이동상 용매를 통해 HPLC 분석을 진행하였다. 분석법의 유효성 검증은 특이성, 직선성, 정확도, 정밀도, 검출한계 및 정량한계 등을 통해 분석법의 신뢰성을 검증하였다. 결과: 작약 뿌리 추출물과 pentaglloylglucose 표준용액 간의 HPLC 크로마토그램 및 UV spectrum의 비교를 통해 다르 물질들의 간섭 없이 피크가 분리된 것으로 특이성을 확인하였으며 상관계수(R2)는 0.9999로 매우 우수한 직선성을 관찰되었다. 검량선의 기울기 및 표준편차를 이용한 검출한계는 0.92 μg/mL였고 정량한계는 2.72 μg/mL으로 확인되었다. 또한, 정확성 분석을 위해 서로 다른 농도 3개를 선정하여 확인한 결과 RSD가 약 0.12-0.50%로 확인되었다. 결론: 이상의 분석결과를 통해 확립된 작약 뿌리 추출물의 지표성분인 pentagalloylglucose의 HPLC 분석법은 적합한 시험법으로 검증되었으며 원료 표준화를 통해 기능성 원료 자료로 활용될 것으로 사료된다.
Abstract
Purpose: This study established a high-performance liquid chromatography (HPLC) method to standardize pentagalloylglucose as a marker compound in Paeonia lactiflora (P. lactiflora) root extracts. Methods: The analytical procedure for standardizing pentagalloylglucose was optimized using HPLC on a C18 column with a gradient elution of 0.1% trifluoroacetic acid and acetonitrile. The method was validated for specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. Results: The method demonstrated high linearity, as evident from a correlation coefficient R2 of 0.9999. The LOD and LOQ were 0.92 and 2.78 μg/mL, respectively. Recovery was determined to be in the range of 101.15 ± 0.49–102.30±0.13, with the relative standard deviation (RSD) values between 0.12%–0.50%, indicating high accuracy. Conclusion: The research lays a foundation for the production of functional foods or cosmetics using pentagalloylglucose derived from P. lactiflora root extract.
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