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Regulation of Colonic Mucosal MicroRNA Expression via Multiple Targets in Visceral Hypersensitivity Rats by Tongxieyaofang

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초록

Purpose: This study aimed to screen for differentially expressed microRNAs (miRNAs) in the colons of rats with visceral hypersensitivityto build the expression profiles of miRNAs therein and to determine the mechanism of Tongxieyaofang use in thetreatment of irritable bowel syndrome (IBS). Materials and Methods: Forty Sprague-Dawley rats were divided randomly into four groups: control group, model control group(induced by rectum stimulus and evaluated by abdominal withdraw reaction), treatment control group (normal saline), andTongxieyaofang group (treated with Tongxieyaofang). We screened for differential expression of colonic mucosal miRNAs usingliquid chip technology and verified the expression thereof using reverse transcription-PCR. Results: The visceral hypersensitivity rat model was successfully established. We found the expression of let-7f, let-7i, miR-130b,miR-29a, miR-132, miR-21, and miR-375 to be up-regulated (p<0.05), while the expression of miR-24, miR-31a, miR-192, miR-221,and miR-223 was down-regulated (p<0.05) in the visceral hypersensitivity rats. After treatment with Tongxieyaofang, the expressionof let-7f, let-7i, miR-130b, miR-29a, miR-132, miR-21, and miR-375 was reduced (p<0.05), whereas the expression of miR-24,miR-31a, miR-192, miR-221, miR-223 was increased, compared to the treatment control group (p<0.05). Conclusion: MiRNAs play a pivotal role in visceral hypersensitivity and might be targets in the treatment of IBS by Tongxieyaofang.

Abstract

Purpose: This study aimed to screen for differentially expressed microRNAs (miRNAs) in the colons of rats with visceral hypersensitivityto build the expression profiles of miRNAs therein and to determine the mechanism of Tongxieyaofang use in thetreatment of irritable bowel syndrome (IBS). Materials and Methods: Forty Sprague-Dawley rats were divided randomly into four groups: control group, model control group(induced by rectum stimulus and evaluated by abdominal withdraw reaction), treatment control group (normal saline), andTongxieyaofang group (treated with Tongxieyaofang). We screened for differential expression of colonic mucosal miRNAs usingliquid chip technology and verified the expression thereof using reverse transcription-PCR. Results: The visceral hypersensitivity rat model was successfully established. We found the expression of let-7f, let-7i, miR-130b,miR-29a, miR-132, miR-21, and miR-375 to be up-regulated (p<0.05), while the expression of miR-24, miR-31a, miR-192, miR-221,and miR-223 was down-regulated (p<0.05) in the visceral hypersensitivity rats. After treatment with Tongxieyaofang, the expressionof let-7f, let-7i, miR-130b, miR-29a, miR-132, miR-21, and miR-375 was reduced (p<0.05), whereas the expression of miR-24,miR-31a, miR-192, miR-221, miR-223 was increased, compared to the treatment control group (p<0.05). Conclusion: MiRNAs play a pivotal role in visceral hypersensitivity and might be targets in the treatment of IBS by Tongxieyaofang.

참고문헌 (38)

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