방사선 기술이용 미생물유래 신규 물성 폴리머 물질 제조 및전도성 검토
Preparation of a Newly Microbial Polymer Using Ionizing Radiation Technique and Its Conductivity
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Extracellular polymeric substance (EPS) is produced by microorganisms for a varietyof purpose in response to environmental stresses. The EPS are heterogeneous mixture composeddominantly of polysaccharides and proteins, with nucleic acid and lipid as minor constituents. Application of a bacterial EPS is in mostly bioremediation in environment and it applied topharmaceutical industries including indwelling medical devices. Furthermore, the EPS studieshave mostly been performed in the only level of bacterial culture system. In this study, wehave first investigated the structural modification of EPS using ionizing radiation technique. The extracted EPS solution from bacterial culture step was exposed to gamma-irradiation atroom temperature. Especially, the EPS was given irradiation at various ranges of 0, 2, 10 and30 kGy from 60Co gamma-ray source. The structural property and surface morphology werecharacterized by using Fourier-transform infrared (FTIR) and field emission-scanning electronmicroscopy (FE-SEM). Also, the conductivity of EPS was determined by using the Van der PauWmethod, and the polymeric substances turned out to have semi-conductivity (about 5.38*10-7).
Abstract
Extracellular polymeric substance (EPS) is produced by microorganisms for a varietyof purpose in response to environmental stresses. The EPS are heterogeneous mixture composeddominantly of polysaccharides and proteins, with nucleic acid and lipid as minor constituents. Application of a bacterial EPS is in mostly bioremediation in environment and it applied topharmaceutical industries including indwelling medical devices. Furthermore, the EPS studieshave mostly been performed in the only level of bacterial culture system. In this study, wehave first investigated the structural modification of EPS using ionizing radiation technique. The extracted EPS solution from bacterial culture step was exposed to gamma-irradiation atroom temperature. Especially, the EPS was given irradiation at various ranges of 0, 2, 10 and30 kGy from 60Co gamma-ray source. The structural property and surface morphology werecharacterized by using Fourier-transform infrared (FTIR) and field emission-scanning electronmicroscopy (FE-SEM). Also, the conductivity of EPS was determined by using the Van der PauWmethod, and the polymeric substances turned out to have semi-conductivity (about 5.38*10-7).
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