Synthesis and Characterization of Manuka Honey Loaded Carboxymethyl Cellulose Hydrogel Prepared by Gamma-ray Irradiation
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Manuka honey differs from normal honey because of its additional existence of nonperoxideantibacterial activity which has been used to treat infections in a wide range of woundtypes. In this study, Manuka honey mixed with carboxymethyl cellulose (CMC) was crosslinkedand sterilized by using a γ-irradiation of 50 kGy from a 60Co source to make a new hydrogelwound dressing. The effects of various parameters such as honey concentration (0~20 wt%), gelcontent, and the absorption ratio in water were investigated in detail. The antibacterial propertieswere evaluated by paper diffusion test. The antibacterial tests indicated that the manuka/CMChydrogels have a good antibacterial activity against Escherichia coli (E. coli) and Staphylococcusaureus (S. aureus). As effective therapeutic evidence, the rate of vivo wound healing process in db/db mice was investigated based on real-time photographs and wound area contraction.
Abstract
Manuka honey differs from normal honey because of its additional existence of nonperoxideantibacterial activity which has been used to treat infections in a wide range of woundtypes. In this study, Manuka honey mixed with carboxymethyl cellulose (CMC) was crosslinkedand sterilized by using a γ-irradiation of 50 kGy from a 60Co source to make a new hydrogelwound dressing. The effects of various parameters such as honey concentration (0~20 wt%), gelcontent, and the absorption ratio in water were investigated in detail. The antibacterial propertieswere evaluated by paper diffusion test. The antibacterial tests indicated that the manuka/CMChydrogels have a good antibacterial activity against Escherichia coli (E. coli) and Staphylococcusaureus (S. aureus). As effective therapeutic evidence, the rate of vivo wound healing process in db/db mice was investigated based on real-time photographs and wound area contraction.
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