감마선 변이체 스프레이 국화 ‘ARTI-purple’의 화색 관련유전자 발현 분석
Expression Analysis of Flower Color Related Genes in Spray-type ‘ARTI-purple’ Developed by Gamma-ray Mutagenesis
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Anthocyanins are major plant pigment and produced through phenylpropanoidpathway. In this study, anthocyanin biosynthesis mechanisms of chrysanthemum flowers werestudied using ‘Argus’ and flower color mutant ‘ARTI-purple’ which were induced by 40 Gygamma irradiation (Co60). And, three chrysanthemums, ‘Ford’, ‘Yeonja’ and ‘Orando’ wereadditionally used as the check varieties to understand the relationship between flower color andexpression patterns of genes. The expression patterns of the anthocyanin biosynthetic genes werematched with the flower color of the check varieties. High anthocyanin concentration of ‘Orando’showed the high expression of anthocyanin biosynthetic genes. In the white flower of ‘Ford’,expressions of CHI, DFR and ANS were not identified. Despite different flower color, ‘Argus’ and‘ARTI-purple’ showed different expression patterns compared with the check varieties. From thedot blot analysis, we screened the seven genes showing the different expressions between ‘Argus’and ‘ARTI-purple’.
Abstract
Anthocyanins are major plant pigment and produced through phenylpropanoidpathway. In this study, anthocyanin biosynthesis mechanisms of chrysanthemum flowers werestudied using ‘Argus’ and flower color mutant ‘ARTI-purple’ which were induced by 40 Gygamma irradiation (Co60). And, three chrysanthemums, ‘Ford’, ‘Yeonja’ and ‘Orando’ wereadditionally used as the check varieties to understand the relationship between flower color andexpression patterns of genes. The expression patterns of the anthocyanin biosynthetic genes werematched with the flower color of the check varieties. High anthocyanin concentration of ‘Orando’showed the high expression of anthocyanin biosynthetic genes. In the white flower of ‘Ford’,expressions of CHI, DFR and ANS were not identified. Despite different flower color, ‘Argus’ and‘ARTI-purple’ showed different expression patterns compared with the check varieties. From thedot blot analysis, we screened the seven genes showing the different expressions between ‘Argus’and ‘ARTI-purple’.
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