Study on the Health Recovery Effects of Injection Omega-3 Fatty Acid to Mouse by Exposing Radiation
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study is to determine the radioprotective effects of Squalene (SQ) extracted fromnatural products, a large amount of which is contained in the liver of centrophorusatromarginatus. For this purpose, SQ was administered to mice exposed to a session of 3 Gy whole-body irradiation;then, first, Caspase-3 and Caspase-9 in the cell signaling pathways, which led to apoptosis, weremeasured to examine cell activity; second, nitric oxide (NO) was measured to observe inflammatoryresponses in cells. There were a total of seven experimental groups; considering the fact that it mighttake the specimens long to be absorbed into living tissues, seven- and three-day factors were appliedto the pretreatment groups and three- and seven-day factors. To determine the radioprotectiveeffects of SQ, a 10 MeV LINAC radiation therapy system was used to expose mice to a session ofwhole-body irradiation in a dose of 3 Gy, which induces the greatest biochemical changes in livingtissues, at the dose rate of 300 cGy·min-1. It has been confirmed that squalene with good antioxidanteffects gives radioprotection by promoting NO generation and by inhibiting Caspase that a naturalproduct with a lower level of chemical toxicity can be used as a radioprotector.
Abstract
This study is to determine the radioprotective effects of Squalene (SQ) extracted fromnatural products, a large amount of which is contained in the liver of centrophorusatromarginatus. For this purpose, SQ was administered to mice exposed to a session of 3 Gy whole-body irradiation;then, first, Caspase-3 and Caspase-9 in the cell signaling pathways, which led to apoptosis, weremeasured to examine cell activity; second, nitric oxide (NO) was measured to observe inflammatoryresponses in cells. There were a total of seven experimental groups; considering the fact that it mighttake the specimens long to be absorbed into living tissues, seven- and three-day factors were appliedto the pretreatment groups and three- and seven-day factors. To determine the radioprotectiveeffects of SQ, a 10 MeV LINAC radiation therapy system was used to expose mice to a session ofwhole-body irradiation in a dose of 3 Gy, which induces the greatest biochemical changes in livingtissues, at the dose rate of 300 cGy·min-1. It has been confirmed that squalene with good antioxidanteffects gives radioprotection by promoting NO generation and by inhibiting Caspase that a naturalproduct with a lower level of chemical toxicity can be used as a radioprotector.
참고문헌 (31)
- Ahn YK, "Effects of Squalene on the Immune Responses in Mice (I): Humoral Immune Responses of Squalene", Arch. Pharmacal Res, 14(4), pp.370~378 (1991)
- Asa PB, "Antibodies to Squalene in Gulf War Syndrome", Exp. Mol. Pathol, 68(1), pp.55~64 (2000)
- Asa PB, "Antibodies to Squalene in Recipients of Anthrax Vaccine", Exp. Mol. Pathol, 73(1), pp.19~27 (2002)
- 배영훈, "토끼의 관절병증에 미치는 알긴산올리고당의 치료효관", 대한수의학회지, 42(2), pp.4~162 (2002), 대한수의학회
- Barrandon Y, "Cell migration is essentiatial for sustained growth of keratinocyte colonies: the role of TGF-alpha and EGF", Cell, 50(7), pp.1131~1137 (1987)
- Bennett NT, "Growth Factors and Wound Healing: Biochemical Properties of Growth Factors", Am. J. Surgery, 165(6), pp.728~737 (1993)
- Byyny R, "Radioimmunoassy of epidermal growth factor", Endocrinol, 90(5), pp.1261~1266
- Clarke GA, "Cholesterol synthesis inhibition distal to squalene upregulates biliary phospholipid secretion and counteracts cholelithiasis in the genetically prone C57L", J mouse, 2004, pp.53(1), 136~142
- Cohen S, "Isolation of a mouse submaxillary gland protein accelrating incisoreruption and eyelid opening in the new-born animal", J. Biol. Chem, 237(5), pp.1555~1562 (1962)
- Cohen S, "Human epidermal growth factor : Isolation and Chemical and Biological properties", Proc. Natl. Acad. Sci, 72(4), pp.1317~1321 (1975)
- Fisher DA, "Metabolism and effects of epidermal growth factor and related growth factors in mannals", Endocr. Rev, 11(3), pp.418~442 (1990)
- Kesaniemi YA, "Cholesterol absorption efficiency regulates plasma cholesterol level in the Finnish population", Eur. J. Clin. Invest, 17(5), pp.391~395 (1987)
- Kim CH, "Effect of Juglandis Semen Herb-Acupuncture (JSA) on Urine Concentrating Defect in Glycerol-Induced Acute Renal Failure", J. Dong-Eui Orient. Med, 7, pp.89~96 (2003)
- Kohno Y, "Kinetic study of quenching reaction of singlet oxygen and scavenging reaction of free radical by squalene in n-butanol", Biochim. Biophys. Acta, 1256(1), pp.52~56 (1995)
- Kuroda Y, "Autoimmunity induced by adjuvant hydrocarbon oil components of vaccine", Biomed. Pharmacother, 58(5), pp.325~337 (2004)
- Lim BS, "Radiation exposure dose on persons engaged in radiation -related industries in Korea", J. Radiol. Sci. Technol, 29(3), pp.185~195 (2006)
- Matyas GR, "Detection of antibodies to squalene III. Naturally occurring antibodies to squalene in humans and mice", J. Immunol. Methods, 286(1-2), pp.47~67 (2004)
- Nakagawa H, "Dose and time-dependence of radiation-induced nitric oxide formation in mice as quantified with electron paramagnetic resonance", Nitric Oxide, 5(1), pp.47~52 (2001)
- Nakamura Y, "Effect of dietary squalene on the fecal steroid excretions and the lipid levels of serum and the liver in the rat", Nutrit. Res, 17(2), pp.243~257 (1997)
- Nanney LB, "Epidermal and dermal effects of epidermal growth factor during wound repair", J. Invest. Dermatol, 94(5), pp.624~629
- Nishimoto T, "Comparing myotoxic effects of squalene synthase inhibitor, T-91485, and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors in human myocytes", Biochem. Pharmacol, 66(11), pp.2133~2139 (2003)
- Relas H, "Dietary squalene increases cholesterol synthesis measured with serum non-cholesterol sterols after a single oral dose in humans", Atherosclerosis, 152(2), pp.377~383 (2000)
- Sabeena Farvin KH, "Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats", Pharmacol. Res, 50(3), pp.231~236 (2004)
- Senthilkumar S, "Effect of squalene on cyclophosphamide-induced toxicity", Clin. Chim. Acta, 364(1-2), pp.335~342 (2006)
- Sethi G, "In vitro activation of murine peritoneal macrophages by ultraviolet B radiation: upregulation of CD18, production of NO, proinflammatory cytokines and a signal transduction pathway", Mol. Immunol, 40(18), pp.1315~1323 (2004)
- Storm HM, "Radioprotection of mice by dietrary squalene", Lipids, 28(6), pp.555~559 (1993)
- Taylor JM, "Epidermal growth factor physical and chemical properties", J. Biol. Chem, 247, pp.5928~5934 (1972)
- Veen M, "Combined overexpression of genes of the ergosterol biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae", FEMS Yeast. Res, 4(1), pp.87~95 (2003)
- Vuorio AF, "Cholesterol metabolism in normal and heterozygous familial hypercholesterolemic newborns", J. Lab. Clin. Med, 140(1), pp.35~42 (2002)
- Wang T, "The role of NF-kB in the regulation of cell stress responses", Int. Immunopharmacol, 2(11), pp.1509~1520 (2002)
- Zaidi AK, "Effect of whole body hyperthermia (39°C, 1h) on radiation-induced apoptosis in Swiss mice", J. Therm. Biol, 29(1), pp.3~8 (2004)