Melatonin Protects Bone Microarchitecture against Deterioration due to High-Fat Diet-Induced Obesity
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초록
Background: We evaluated the protective effects of melatonin against high-fat diet (HFD)-induced deterioration of bone microarchitecture using high-resolution peripheral quantitative computed tomography (HR-pQCT). Methods: Four-week-old male C57BL/6 mice were divided into control (chow diet group), HFD, and HFD + melatonin-administered groups. Mice were sacrificed after 14 weeks, and the right femur was extracted. The microskeletal structure of the femur was analyzed using SkyScan1173 (version 1.6). A 3-dimensional image was reconstructed using the Nrecon (version 1.7.0.4) program. Results: Bone volume (BV) was significantly increased in the HFD group compared with that in the normal diet group, and that of the melatonin group also increased significantly compared with BV of the normal diet group (P<0.05). Percent BV/total volume [TV] and bone surface/BV were significantly higher in both the HFD and melatonin groups than in the normal diet group (P<0.05), and the melatonin group had the highest BV/total volume (TV). BMD was lower in the HFD than in the normal diet group and was the highest in the melatonin group. Conclusions: This study shows that melatonin inhibited the deterioration of microarchitecture induced by a HFD. A better understanding of the protective effect of melatonin on bone microarchitecture and mechanisms could provide fracture prevention for people who are obese.
Abstract
Background: We evaluated the protective effects of melatonin against high-fat diet (HFD)-induced deterioration of bone microarchitecture using high-resolution peripheral quantitative computed tomography (HR-pQCT). Methods: Four-week-old male C57BL/6 mice were divided into control (chow diet group), HFD, and HFD + melatonin-administered groups. Mice were sacrificed after 14 weeks, and the right femur was extracted. The microskeletal structure of the femur was analyzed using SkyScan1173 (version 1.6). A 3-dimensional image was reconstructed using the Nrecon (version 1.7.0.4) program. Results: Bone volume (BV) was significantly increased in the HFD group compared with that in the normal diet group, and that of the melatonin group also increased significantly compared with BV of the normal diet group (P<0.05). Percent BV/total volume [TV] and bone surface/BV were significantly higher in both the HFD and melatonin groups than in the normal diet group (P<0.05), and the melatonin group had the highest BV/total volume (TV). BMD was lower in the HFD than in the normal diet group and was the highest in the melatonin group. Conclusions: This study shows that melatonin inhibited the deterioration of microarchitecture induced by a HFD. A better understanding of the protective effect of melatonin on bone microarchitecture and mechanisms could provide fracture prevention for people who are obese.
참고문헌 (30)
- Poirier P, "Obesity and cardiovascular disease: pathophysiology, evaluation, and effect of weight loss: an update of the 1997 American Heart Association Scientific Statement on Obesity and Heart Disease from the Obesity Committee of the Council on Nutrition, Physical Activity, and Metabolism", Circulation, 113, pp.898~918 (2006)[DOI]
- Iyengar NM, "Obesity and cancer mechanisms : tumor microenvironment and inflammation", J Clin Oncol, 34, pp.4270~4276 (2016)[DOI]
- 남가은, "Obesity Fact Sheet in Korea, 2019: Prevalence of Obesity and Abdominal Obesity from 2009 to 2018 and Social Factors", Journal of Obesity & Metabolic Syndrome, 29(2), pp.124~132 (2020), 대한비만학회[DOI]
- Arroyo-Johnson C, "Obesity epidemiology worldwide", Gastroenterol Clin North Am, 45, pp.571~579 (2016)[DOI]
- Abdelaal M, "Morbidity and mortality associated with obesity", Ann Transl Med, 5, pp.161~ (2017)[DOI]
- 남가은, "Obesity Fact Sheet in Korea, 2020: Prevalence of Obesity by Obesity Class from 2009 to 2018", Journal of Obesity & Metabolic Syndrome, 30(2), pp.141~148 (2021), 대한비만학회[DOI]
- Curtis EM, "Epidemiology of fractures in the United Kingdom 1988-2012: Variation with age, sex, geography, ethnicity and socioeconomic status", Bone, 87, pp.19~26 (2016)[DOI]
- 안승희, "Osteoporosis and Osteoporotic Fracture Fact Sheet in Korea", 대한골대사학회지, 27(4), pp.281~290 (2020), 대한골대사학회
- Guzon-Illescas O, "Mortality after osteoporotic hip fracture: incidence, trends, and associated factors", J Orthop Surg Res, 14, pp.203~ (2019)[DOI]
- Park SM, "Incidence and mortality of subsequent vertebral fractures : analysis of claims data of the Korea National Health Insurance Service from 2007 to 2016", Spine J, 20, pp.225~233 (2020)[DOI]
- Shiomoto K, "Effect of body mass index on vertebral and hip fractures in older people and differences according to sex : a retrospective Japanese cohort study", BMJ Open, 11, pp.e049157~ (2021)[DOI]
- Soliman SS, "The impact of BMI on morbidity and mortality after femoral fractures", Eur J Trauma Emerg Surg, 48, pp.2441~2447 (2022)[DOI]
- Roden M, "Mechanisms of Disease : hepatic steatosis in type 2 diabetes--pathogenesis and clinical relevance", Nat Clin Pract Endocrinol Metab, 2, pp.335~348 (2006)[DOI]
- Lee SJ, "Obesity and bone health revisited : A mendelian randomization study for Koreans", J Bone Miner Res, 34, pp.1058~1067 (2019)[DOI]
- Ross DS, "Distinct effects of a high fat diet on bone in skeletally mature and developing male C57BL", 6J mice, 2021, pp.13, ~
- Freid R, "Tributyltin protects against ovariectomy-induced trabecular bone loss in C57BL", 6J mice with an attenuated effect in high fat fed mice, 2021, pp.431, 115736~
- Lu X, "Insight into the roles of melatonin in bone tissue and bone‑related diseases(Review)", Int J Mol Med, 47 (2021)[DOI]
- Liu PI, "Melatonin interrupts osteoclast functioning and suppresses tumor-secreted RANKL expression : implications for bone metastases", Oncogene, 40, pp.1503~1515 (2021)[DOI]
- Han Y, "Melatonin promotes osteoblast differentiation by regulating Osterix protein stability and expression", Sci Rep, 7, pp.5716~ (2017)[DOI]
- Vos T, "Years lived with disability(YLDs)for 1160 sequelae of 289 diseases and injuries 1990-2010 : a systematic analysis for the Global Burden of Disease Study 2010", Lancet, 380, pp.2163~2196 (2012)[DOI]
- Sarafrazi N, "Osteoporosis or low bone mass in older adults: United States, 2017-2018", NCHS Data Brief, 2021, pp.1~8 (2021)
- Egermann M, "Pinealectomy affects bone mineral density and structure--an experimental study in sheep", BMC Musculoskelet Disord, 12, pp.271~ (2011)[DOI]
- Bizot-Espiard JG, "Diurnal rhythms in plasma glucose, insulin, growth hormone and melatonin levels in fasted and hyperglycaemic rats", Diabetes Metab, 24, pp.235~240 (1998)
- Cipolla-Neto J, "Melatonin, energy metabolism, and obesity: a review", J Pineal Res, 56, pp.371~381 (2014)[DOI]
- Munmun F, "Melatonin effects on bone : Implications for use as a therapy for managing bone loss", J Pineal Res, 71, pp.e12749~ (2021)[DOI]
- Kim S, "Osteoblastic glucocorticoid signaling exacerbates high-fat-diet-induced bone loss and obesity", Bone Res, 9, pp.40~ (2021)[DOI]
- Jing H, "Suppression of EZH2 prevents the shift of osteoporotic MSC fate to adipocyte and enhances bone formation during osteoporosis", Mol Ther, 24, pp.217~229 (2016)[DOI]
- Masanova V, "Effects of ovariectomy and exercise training on mineral status in a high-fat diet-induced obesity rat model", Biol Trace Elem Res, 200, pp.624~634 (2022)[DOI]
- Gagnon C, "Osteoporosis in men : its pathophysiology and the role of teriparatide in its treatment", Clin Interv Aging, 3, pp.635~645 (2008)[DOI]
- Andersen S, "Bone structure and estimated bone strength in obese patients evaluated by high-resolution peripheral quantitative computed tomography", Calcif Tissue Int, 95, pp.19~28 (2014)[DOI]