Anti-wrinkle Efficacy of Oenothera laciniata Methanol Extract in Human Dermal Fibroblasts
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The objective of this study was to evaluate the anti-wrinkle efficacy of Oenothera laciniata methanol extract (OLME) in vitro using human dermal fibroblasts. Specifically, we tested the type-I procollagen synthetic ability of OLME at concentrations of 6.25, 12.5, 25, and 50 μg/mL and noted significantly significant (p<0.05) increases versus the control of 38.7, 74.3, 111.4, and 113.0%, respectively. Using RT-PCR, the expression of MMP-1 mRNA in human dermal fibroblasts treated with 6.25, 12.5, 25, and 50 μg/mL OLME was shown to have decreased when compared to the control by 67.7, 71.7, 75.6, and 84.3%, respectively (p<0.05). When measuring MMP-2 activity in human dermal fibroblasts treated with 6.25, 12.5, 25, and 50 μg/mL OLME using zymography, it was shown to have decreased versus the control by 27.6, 37.1, 76.3, and 93.9%, respectively (p<0.05). OLME was less cytotoxic to human dermal fibroblasts than ascorbic acid. From these results, we found that OLME is highly efficacious in its ability to promote collagen synthesis and inhibit its degradation in human dermal fibroblasts, similar or even superior to those of ascorbic acid, a well-known anti-skin aging agent. Therefore, OLME could be used as a functional cosmetic material for the prevention or alleviation of skin wrinkles induced by ultraviolet rays.
Abstract
The objective of this study was to evaluate the anti-wrinkle efficacy of Oenothera laciniata methanol extract (OLME) in vitro using human dermal fibroblasts. Specifically, we tested the type-I procollagen synthetic ability of OLME at concentrations of 6.25, 12.5, 25, and 50 μg/mL and noted significantly significant (p<0.05) increases versus the control of 38.7, 74.3, 111.4, and 113.0%, respectively. Using RT-PCR, the expression of MMP-1 mRNA in human dermal fibroblasts treated with 6.25, 12.5, 25, and 50 μg/mL OLME was shown to have decreased when compared to the control by 67.7, 71.7, 75.6, and 84.3%, respectively (p<0.05). When measuring MMP-2 activity in human dermal fibroblasts treated with 6.25, 12.5, 25, and 50 μg/mL OLME using zymography, it was shown to have decreased versus the control by 27.6, 37.1, 76.3, and 93.9%, respectively (p<0.05). OLME was less cytotoxic to human dermal fibroblasts than ascorbic acid. From these results, we found that OLME is highly efficacious in its ability to promote collagen synthesis and inhibit its degradation in human dermal fibroblasts, similar or even superior to those of ascorbic acid, a well-known anti-skin aging agent. Therefore, OLME could be used as a functional cosmetic material for the prevention or alleviation of skin wrinkles induced by ultraviolet rays.
참고문헌 (22)
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