자귀나무(Albizzia julibrissin) 잎 추출물의 항주름 및 항산화 활성
Anti-wrinkle and Antioxidant Activity of the Extract of Albizzia julibrissin Leaves
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최근 민간이나 한의학에서 질병 치료 및 예방의 목적으로사용되어 안전성과 안정성이 입증된 약용식물과 이의 부산물로부터 유용성분을 추출하여 기능성 식품이나 의약품 및화장품의 소재로 활용하기 위해 많은 연구가 이루어지고 있다. 이에 자귀나무 잎을 물과 에탄올을 용매로 추출하여 각추출물에 대한 유용성분의 함량과 항노화 및 항산화 활성을측정하고 천연물 유래 소재로서 활용 가능성을 알아보고자하였다. 건조된 자귀나무 잎의 물 추출물(ALW)은 플라보노이드76.42 mg/g과 폴리페놀 153.67 mg/g을 함유하였으며, 에탄올추출물인 ALE는 각각 99.40 mg/g과 154.32 mg/g을 함유하였다. Xanthine oxidase 저해 효과는 2,000 μg/mL의 농도에서96%로 대조군인 ascorbic acid와 자귀나무 추출물간의 유의적 차이가 없었으나 IC50은 ascorbic acid 41.60 μg/mL, ALE49.31 μg/mL 그리고 ALW 60.14 μg/mL의 농도에서 50%의저해효과를 나타내었다. 항주름 효과를 나타내는 elastase와collagenase 저해 효과는 2,000 μg/mL에서 ALW가 23.46%과48.23%로 ALE보다 높은 저해활성을 나타내었으나 미백활성을 나타내는 tyrosinase 저해율은 ALE가 21.72%로 ALW(12.72%)보다 높은 활성을 나타내었다. 아질산염 소거능은모든 pH 조건에서 ascorbic acid보다 낮았으며, 2,000 μg/mL의 pH 1.2에서 ALE는 78.03%였으며 SC50은 578.78 μg/mL이었다. SOD 유사활성은 ALW 25.89%, ALE 28.97%였으며,DPPH radical 소거는 각각 89.14%와 95.94%로 SC50은118.68 μg/mL와 81.98 μg/mL를 나타내었다. 이상의 결과로미루어보아 항주름 효과는 물을 용매로 추출한 ALW가 에탄올을 추출물인 ALE보다 좀 더 우수한 저해효과를 보였으며, 미백효과와 항산화 활성은 ALE가 좀더 높은 활성을 나타내었다. 이상의 결과 자귀나무 잎은 합환피라는 한약재명으로 한약재료로 사용되고 있는 자귀나무 수피보다 많은 플라보노이드와 폴리페놀 화합물을 함유하며, 자귀나무 수피보다 우수한 96%의 xanthine oxidase 저해와 약 80%의 아질산염소거 및 약 90% 이상의 DPPH radical 소거활성을 나타내어자귀나무 잎 추출물은 화장품 및 이와 관련된 향장산업의기능성 소재로서 활용 가능할 것으로 판단된다.
Abstract
In the current study, extract from the leaves of Albizzia julibrissin was prepared using either water (ALW) or 80%ethanol (ALE). The total flavonoid and polyphenol compound contents, the anti-wrinkle and whitening effects, and theantioxidant activities of the organic solvent fractions from the extracts were investigated. The total flavonoid andpolyphenol compound contents of ALE were 99.40 mg/g and 154.32 mg/g, respectively. The inhibition of xanthineoxidase found for ALW and ALE at 2.0 mg/mL were both over 96%, and there were no significant differences in ascorbicacid. At a concentration of 2,000 μg/mL, the levels of elastase and collagenase inhibition underlying the anti-wrinkleeffect of ALW were 23.46% and 48.23%, respectively. The level of tyrosinase inhibition responsible for whiteningactivity was 21.72% in ALE. Additionally, the nitrite scavenging ability of ALE at pH 1.2, was 78.03% and the SC50 wascalculated at 578.78 μg/mL. The superoxide dismutase-like activity of ALE at 2.0 mg/mL was 28.97%, and the 1,1-diphenyl-2-picrylhydrazyl radical activity of ALE was 95.94%. Furthermore, the SC50 values for ALW and ALE were118.68 μg/mL and 81.98 μg/mL, respectively. These results suggested that the extract from the leaves of A. julibrissinmay be considered as a potential resource for the development of functional cosmetic materials.
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