Tocopheryl Acetate를 함유한 고형 지질 나노입자 제조와 봉입 효율 분석
Preparation of Solid Lipid Nanoparticle Containing Tocopheryl Acetate and Its Encapsulation Efficiency
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본 연구는 고형 지질 나노입자 (SLN)를 용융분산법 (melt dis- persion technique)으로 제조하여, 사용된 계면활성제의 종류에 따른 입자 형상, 입자 크기, 안정성 및 유효물질의 봉입 효율을 측정하여 다음과 같은 주요 결과를 얻었다. 입자의 크기는 계면활성제 Tween 20, 1 wt% 농도를 교반 속 도 1,000 rpm으로 회전시킨 후 25°C에서 분산시켜 제조된 SLN 은 980 nm로 입자 크기가 가장 컸고 Tween 80, 1 wt% 교반 속도 2,000 rpm으로 회전시킨 후, 4°C에서 분산시켜 제조된 SLN은 303 nm로 입자 크기가 가장 작았다. 제타전위 값은 제조한 모든 SLN에서 안정성을 보였으며, 계면활성제의 종류와 농도 변화 에 큰 영향을 받지 않았으나 온도에는 영향을 받아 4°C에서 25 °C보다 안정성이 높게 나타났다. 입자 형상은 계면활성제의 종 류에 따라 Tween 20, 1 wt%와 Tween 80, 1 wt% 및 4 wt%의 SLN 에서 정형화된 과립형의 형상을 나타내었고, Tween 80의 경우 회전속도가 증가 할수록 입자 크기는 작고 형상은 구형에 가깝 게 나타났다. 봉입 효율은 Tween 20, 10 wt%를 교반 속도 1,000 rpm으로 회전시킨 후 25°C에서 분산시킨 SLN이 방출률 77.1% 이었으며 봉입 효율은 22.9%로 가장 낮았고 Tween 80, 4 wt%를 교반 속도 2,000 rpm으로 회전시킨 후, 4°C에서 분산시킨 SLN 은 방출률 19.2%이었으며 봉입 효율은 80.8%로 가장 우수하였 다. 따라서 SLN의 효능물질 봉입 효율은 계면활성제의 종류와 분산 온도, 지질과 계면활성제의 적정 혼합비에 영향을 받는 것 으로 판단된다.
Abstract
Various forms of cosmetics have been studied in order to increase the skin penetration rate of active ingredients. Solid lipid nanoparticles (SLNs) that can continuously release an active ingredient for many days by encapsulating a drug in polymer nanoparticles were prepared and analyzed to determine encapsulation efficiency by: i) the type and quantity of surfactant, ii) agitation speed, and iii) dispersion temperature. To prepare SLNs encapsulating tocopheryl acetate, the concentration of stearic acid was fixed to 1 wt%, the concentrations of surfactants (Tween 20 and Tween 80) studied were 1, 4, and 10 wt%, the agitation speeds tested were 1,000 rpm and 2,000 rpm, and the dispersion temperatures used were 4°C and 25°C. The 1 wt% Tween 20, along with 1 wt% and 4 wt% Tween 80 SLNs showed standardized granular shapes, and 1 wt% Tween 80 SLN at 4°C and 2,000 rpm showed the smallest particle size of 303 nm. The zeta potential in all SLNs produced ranged from -20.4 mV to -39.3 mV, showing a high degree of stability, while 4 wt% Tween 80 SLN at 4°C and 2,000 rpm showed the highest encapsulation efficiency of 80.8%. Therefore, it is confirmed that both the type of surfactant used and the dispersion temperature, along with the mix ratio of lipid and surfactant are important factors for the production of SLNs with high skin absorption rates.
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