인체 피지선 세포에서 그라비올라 잎 추출물의 피지생성 억제 효과
Inhibitory Effect of Graviola Leaf Extract on Lipid Synthesis in Human Sebocyte Cells
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본 연구에서는, 그라비올라 잎 추출물의 인체 피지선 세포에서 피지생성에 미치는 영향을 알아보기 위해, 피지선 세포에 그라비올라 잎 추출물을 처리한 후, 피지선 세포에서 지방생성과 지방생성 관련 분자들을 확인하였다. 인체 피지선 세포에서 그라비올라 잎 추출물은 농도 의존적으로 지방생성을 농도 의존적으로 억제하였다. 대조군에 비해 그라비올라 잎 추출물 100, 300, 500 μg/mL을 처리하였을 때, 각각 11.6%, 21.0%, 34.5% 지방생성이 억제되었다. 또한 콜레스테롤 생성 억제 효과는 그라비올라 잎 추출물 100, 300, 500 μg/mL에서 각각 13.1%, 22.4%, 37.5% 억제되었다. 그리고 중성지방 생성 억제 효과는 그라비올라 잎 추출물 100, 300, 500 μg/mL에서 각각 9.8%, 22.2%, 41.6%로 나타났다. 지방생성에 관여하는 것으로 알려진 전사인자 활성을 확인한 결과 그라비올라 잎 추출물에 의하여 C/EBP-α, PPAR-γ, SREBP-1의 활성이 억제됨을 확인하였다. 또한 이와 관련된 단백질인 EKR, Akt의 발현을 조사한 결과, 추출물에 의하여 발현이 감소됨을 확인하였다. 본 연구를 통해, 그라비올라 잎 추출물은 인체 피지선 세포에서 피지생성을 억제하는 효능을 확인하였고, 효능을 피지생성에 관련된 전사인자와 단백질 발현의 억제를 통하여 일어남을 확인하였다.
Abstract
The main function of the human sebaceous gland is sebum excretion. Increased sebum levels combined with follicular hyperkeratinization are prerequisites of acne vulgaris. Some transcription factors have been identified, such as peroxisome proliferator-activated receptors (PPARs), CCAAT/enhancer binding proteins (c/EBPs) and sterol regulatory element-binding protein 1 (SREBP-1), which are known to control lipid metabolism in adipocytes. In the current study, we investigated the anti-inhibitory effect of Graviola leaf extract (GLE) on lipid synthesis in human sebocytes. Treatment with GLE significantly suppressed lipid content of human sebocytes in a dose-dependent manner. The concentrations of cellular triglyceride and cholesterol were also reduced in human sebocyte after treatment with GLE when compared with the controls. In addition, GLE also reduced the expression levels of the transcription factors C/EBP-1, PPAR-γ and SREBP-1 significantly. Furthermore, GLE modulated the phosphorylation of ERK and Akt signal molecules. Taken together, these findings revealed that GLE showed strong anti-inhibitory effects on lipid synthesis in human sebocytes.
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