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조협 추출물이 HaCaT cells의 항염증과 SEB-1 cells의 피지분비 억제에 미치는 영향

The Effects on Anti-inflammatory Action in HaCaT Cells and Inhibiting Sebum Secretion in SEB-1 Cells by Gleditsiae Fructus Extract

김윤희|한재경|구은진
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2016.05|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제30권 제2호|pp. 96-106

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키워드

TriglycerideAcneSebumInflammationRANTESHaCaT cellsGleditsiae fructusSEB-1 cellsIP-10MDC
주제분류한의학

초록

Objectives The purpose of this study is to investigate the effects of Gleditsiae Fructus 70% EtOH extract (JS_E) on anti-inflammatory action in HaCaT cells (A spontaneously immortalized human keratinocyte cell line) and inhibiting triglyceride genesis in SEB-1 cells (Immortalized human sebocyte). Methods The anti-inflammatory effect of JS_E was analyzed by enzyme-linked immunosorbent assays (ELISA) which measured levels of IP-10, RANTES and MDC in HaCaT cells. Also the effect on secretion of sebum of JS_E was analyzed by TG-S kit which measured the quantity of triglyceride in SEB-1 cells. Results JS_E inhibited IP-10, RANTES and MDC expression in a dose dependent manner. IP-10 expression was inhibited significantly in comparison to TNF-α and IFN-γ recombination (TI) control group at concentration of JS_E 200 ㎍/㎖ and RANTES and MDC expressions were inhibited significantly at concentration of JS_E 100, 200 ㎍/㎖. JS_E also inhibited triglyceride secretion of SEB-1 cells significantly in comparison to the control group in a dose dependent manner. Conclusions This study shows that JS_E has the effects of anti-inflammatory action and inhibiting sebum secretion. According to these results, JS_E can be used for treating skin diseases such as acne and dermatitis caused by inflammation and excessive secretion of sebum by controlling the activity of the HaCaT and SEB-1 cells.

Abstract

Objectives The purpose of this study is to investigate the effects of Gleditsiae Fructus 70% EtOH extract (JS_E) on anti-inflammatory action in HaCaT cells (A spontaneously immortalized human keratinocyte cell line) and inhibiting triglyceride genesis in SEB-1 cells (Immortalized human sebocyte). Methods The anti-inflammatory effect of JS_E was analyzed by enzyme-linked immunosorbent assays (ELISA) which measured levels of IP-10, RANTES and MDC in HaCaT cells. Also the effect on secretion of sebum of JS_E was analyzed by TG-S kit which measured the quantity of triglyceride in SEB-1 cells. Results JS_E inhibited IP-10, RANTES and MDC expression in a dose dependent manner. IP-10 expression was inhibited significantly in comparison to TNF-α and IFN-γ recombination (TI) control group at concentration of JS_E 200 ㎍/㎖ and RANTES and MDC expressions were inhibited significantly at concentration of JS_E 100, 200 ㎍/㎖. JS_E also inhibited triglyceride secretion of SEB-1 cells significantly in comparison to the control group in a dose dependent manner. Conclusions This study shows that JS_E has the effects of anti-inflammatory action and inhibiting sebum secretion. According to these results, JS_E can be used for treating skin diseases such as acne and dermatitis caused by inflammation and excessive secretion of sebum by controlling the activity of the HaCaT and SEB-1 cells.

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