식용곤충 쌍별귀뚜라미 동충하초 에탄올 추출물의 산화적스트레스에 대한 피부보호 효과 연구

Abstract

With the increase in environmental pollutants and oxidative stress, there is an increased interest in identifying natural bioactive compounds with antioxidant and skin-protective properties. Our study investigates the protective effects of the ethanol extract of the edible insect Gryllus bimaculatus-Cordyceps militaris against oxidative stress- induced damage on human keratinocytes (HaCaT). The extract was prepared using 70% ethanol and the phenolic and flavonoid contents were analyzed. Antioxidant activity was evaluated through (2,2-diphenyl-1-picrylhydrazyl) DPPH and 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS radical scavenging assays. Cytotoxicity was assessed using an (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) MTT assay on HaCaT keratinocyte cells. Potential anti-aging properties were determined by measuring the inhibitory effects on elastase and collagenase through the analysis of the expression of matrix metalloproteinase-1 (MMP-1) and hyaluronic acid (HA) synthesis. The extract contained significant amounts of total phenolics (26.62 mg GAE/g) and flavonoids (0.372 mg CE/g). It exhibited strong radical scavenging activity (DPPH: 62.75%, ABTS: 40.51%). There was no cytotoxicity at tested concentrations in the extract with effective inhibition of elastase (42.6%) and collagenase (52.1%) activity. MMP-1 expression was significantly reduced and HA synthesis was enhanced by up to 90%, which suggested improved skin hydration. Our findings establish that the Gryllus bimaculatus-Cordyceps militaris extract possesses antioxidant, anti- aging, and moisturizing effects, which makes it a promising candidate for natural skincare applications.