보습제 원료의 세포자극성 및 피부보습에 미치는 정도에 관한 연구

Abstract

Due to the externally environmental changes and the internal factors, as the dryness in the skin becomes serious and as the drying inflammation in the skin, and others gradually increase, the natural extracts with moisturizing constituents were developed as products and are being used, together with its research and development, but it is insignificant in a study on the skin irritation and on the availability for actual moisture. Accordingly, this study was examined limitations that do not indicate irritation, after passing through the cell (L929) irritation test in some natural extracts, which were known as moisturizing constituents, and inquired into the influence upon actual moisture, by using lotion category of complete product as sample, based on the application potential for product development. As natural extracts, Urea, Sodium PCA, and GF-glucan are being evaluated to be very excellent as the skin moisturizers. In terms of the cell-irritation experiment that used L929 cell for Urea, Sodium PCA, and GF-glucan, which are these natural extracts, in the concentration of Urea 30 mg/ml (0.3%), the toxicity of 44.8% was indicated, and Sodium PCA represented the cytotoxic activity in all the concentration, except the concentration of 3 ul, due to the function of separating the cell attached by the effect of its viscosity. GF-glucan indicated the cytotoxic activity of 3.1% in 0.1% solution of 1mg/ml, thus it was not relatively irritative, and in the moisture-maintenance measurement, the sample, which added Urea of 2%, did not indicate significance, but the sample, which added Urea of 5% and 10%, represented the moisture-maintenance condition that exceeds one hour. Sodium PCA could be seen the moisture significance of more than one hour in all the samples that added 2%, 5%, and 10%, respectively, but GF-glucan could not be seen the significance in moisture maintenance of more than 20 minutes in terms of the sample that added 5% and 10%, respectively. Given synthesizing and seeing these results, Urea, Sodium PCA, and GF-glucan suggest the availability for moisturizer in natural extracts, and are thought to have the potentiality for being utilized as the moisturizer that is not relatively irritative.