제2형 당뇨모델 생쥐에서 치주인대조직 내 항상성조절인자 변화: 예비연구
Altered homeostatic factor of db/db mice’s periodontal ligament: A pilot study
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Purpose: Diabetes is a risk factor for periodontal disease and can cause alveolar bone loss. The purpose of this study was to explore the effect of diabetes on homeostatic factor of periodontal tissue using leptin receptor (db/db) mice. Materials and Methods: The db/db mice(experimental group) were compared with the wild type mice (control group) using histology and immunohistochemical analyses. Collagen fibers were evaluated using H&E, picrosirius red, fibromodulin staining and osteogenic factors (periostin, osteopontin, osteocalcin, osterix), and factors related to osteoclasts (OPG, RANKL) in the PDL were investigated. Results: Collagen fibers and osteogenic factors in the PDL showed no differences between a control and an experimental group. In an experimental group, expression of receptor activator of nuclear factor kappa-Β ligand (RANKL) was similar compared with a control group, while expression of osteoprotegerin (OPG) was decreased. Conclusions: RANKL/OPG ratio may be one of homeostatic factors related to bone remodeling in diabetic mice.
Abstract
Purpose: Diabetes is a risk factor for periodontal disease and can cause alveolar bone loss. The purpose of this study was to explore the effect of diabetes on homeostatic factor of periodontal tissue using leptin receptor (db/db) mice. Materials and Methods: The db/db mice(experimental group) were compared with the wild type mice (control group) using histology and immunohistochemical analyses. Collagen fibers were evaluated using H&E, picrosirius red, fibromodulin staining and osteogenic factors (periostin, osteopontin, osteocalcin, osterix), and factors related to osteoclasts (OPG, RANKL) in the PDL were investigated. Results: Collagen fibers and osteogenic factors in the PDL showed no differences between a control and an experimental group. In an experimental group, expression of receptor activator of nuclear factor kappa-Β ligand (RANKL) was similar compared with a control group, while expression of osteoprotegerin (OPG) was decreased. Conclusions: RANKL/OPG ratio may be one of homeostatic factors related to bone remodeling in diabetic mice.
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