외부 무기인 농도 변화에 의해 조절되는 pst pit 돌연변이 살모넬라주의 arg 및 art 유전자 전사
Transcriptional expression of arg and art genes in pst pit mutants of Salmonella Typhimurium according to extracellular inorganic phosphate levels
4
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Salmonella enterica serovar Typhimurium (S. Typhimurium) can cause salmonellosis, including gastroenteritis and systemic infection in animal hosts such as humans by diet contamination. To replicate and survive in an animal host, S. Typhimurium must compete against host cells for nutrients. Inorganic phosphate (Pi) is an essential element for all organisms and is used for cellular signal transduction and synthesis of various biomolecules. Thus, systems for uptake and homeostasis of Pi are required for cell metabolism. Uptake of Pi in S. Typhimurium is mainly regulated by components of the high-affinity adenosine triphosphate-binding cassette-transport system Pst and low-affinity transporter Pit. To identify genetic factors contributing to homeostasis of Pi, this study investigated the transcriptional profiles of Salmonella mutants lacking Pst and Pit transporters experiencing changes in extracellular Pi. Materials and Methods: Transcriptome of pst pit mutant S. Typhimurium in response to low (0.1 mM) and high (10 mM) extracellular Pi were compared by cDNA microarray analysis, respectively. Results: The results show that transcription of argI encoding ornithine carbamoyltransferase and artJ encoding arginine third transport system was significantly induced. Conclusion: Data suggest a relationship between arginine metabolism and Pi homeostasis in S. Typhimurium.
Abstract
Purpose: Salmonella enterica serovar Typhimurium (S. Typhimurium) can cause salmonellosis, including gastroenteritis and systemic infection in animal hosts such as humans by diet contamination. To replicate and survive in an animal host, S. Typhimurium must compete against host cells for nutrients. Inorganic phosphate (Pi) is an essential element for all organisms and is used for cellular signal transduction and synthesis of various biomolecules. Thus, systems for uptake and homeostasis of Pi are required for cell metabolism. Uptake of Pi in S. Typhimurium is mainly regulated by components of the high-affinity adenosine triphosphate-binding cassette-transport system Pst and low-affinity transporter Pit. To identify genetic factors contributing to homeostasis of Pi, this study investigated the transcriptional profiles of Salmonella mutants lacking Pst and Pit transporters experiencing changes in extracellular Pi. Materials and Methods: Transcriptome of pst pit mutant S. Typhimurium in response to low (0.1 mM) and high (10 mM) extracellular Pi were compared by cDNA microarray analysis, respectively. Results: The results show that transcription of argI encoding ornithine carbamoyltransferase and artJ encoding arginine third transport system was significantly induced. Conclusion: Data suggest a relationship between arginine metabolism and Pi homeostasis in S. Typhimurium.
참고문헌 (23)
- Kim KS, "Inorganic polyphosphate is essential for long-term survival and virulence factors in Shigella and Salmonella spp", Proc Natl Acad Sci U S A, 99, pp.7675~7680 (2002)
- Vazquez-Torres A, "Oxygen-dependent anti-Salmonella activity of macrophages", Trends Microbiol, 9, pp.29~33 (2001)
- Lamarche MG, "The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis", FEMS Microbiol Rev, 32, pp.461~473 (2008)
- Rice CD, "Employment of a promoter-swapping technique shows that PhoU modulates the activity of the PstSCAB2 ABC transporter in Escherichia coli", Appl Environ Microbiol, 75, pp.573~582 (2009)
- Motomura K, "Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation", FEMS Microbiol Lett, 320, pp.25~32 (2011)
- Hsieh YJ, "Global regulation by the seven-component Pi signaling system", Curr Opin Microbiol, 13, pp.198~203 (2010)
- Crépin S, "The Pho regulon and the pathogenesis of Escherichia coli", Vet Microbiol, 153, pp.82~88 (2011)
- van Veen HW, "Phosphate transport in prokaryotes: molecules, mediators and mechanisms", Antonie Van Leeuwenhoek, 72, pp.299~315 (1997)
- Vershinina OA, "The Pho regulons of bacteria", Mikrobiologiia, 71, pp.581~595 (2002)
- Jiang W, "Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2", J Bacteriol, 177, pp.6411~6421 (1995)
- Surin BP, "Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships", J Bacteriol, 161, pp.189~198 (1985)
- Aguena M, "Transcriptional analysis of the pst operon of Escherichia coli", Mol Genet Genomics, 268, pp.518~524 (2002)
- Hoffer SM, "The phosphate-binding protein of Escherichia coli is not essential for P(i)-regulated expression of the pho regulon", J Bacteriol, 183, pp.5768~5771 (2001)
- Rao NN, "Inorganic polyphosphate in Escherichia coli: the phosphate regulon and the stringent response", J Bacteriol, 180, pp.2186~2193 (1998)
- Gebhard S, "The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis", BMC Microbiol, 9, pp.254~ (2009)
- Jackson RJ, "Expression of the PitA phosphate", metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels, 2008, pp.289, 219~224
- Cunin R, "Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12", Nucleic Acids Res, 11, pp.5007~5019 (1983)
- Charlier D, "Arginine regulon of Escherichia coli K-12. A study of repressor-operator interactions and of in vitro binding affinities versus in vivo repression", J Mol Biol, 226, pp.367~386 (1992)
- Charlier D, "The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation", Microbiology, 152, pp.3343~3354 (2006)
- Rajagopal BS, "Use of inducible feedback-resistant N-acetylglutamate synthetase (argA) genes for enhanced arginine biosynthesis by genetically engineered Escherichia coli K-12 strains", Appl Environ Microbiol, 64, pp.1805~1811 (1998)
- Eckey V, "Studying subunit-subunit interactions in a bacterial ABC transporterby in vitro assembly", Biochim Biophys Acta, 1798, pp.1250~1253 (2010)
- Vahedi-Faridi A, "Crystal structures and mutational analysis of the arginine-, lysine-, histidine-binding protein ArtJ from Geobacillus stearothermophilus. Implications for interactions of ArtJ with its cognate ATP-binding cassette transporter, Art(MP)2", J Mol Biol, 375, pp.448~459 (2008)
- Rao NN, "Molecular aspects of phosphate transport in Escherichia coli", Mol Microbiol, 4, pp.1083~1090 (1990)