우렁이 기능성 활성물질의 고효율 추출조건 구명
Analysis of the Optimal Extraction Condition for the Functional Principles of the Apple Snail
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초록
우렁이에서 유래한 콘드로이틴 설페이트를 새로운 기능성 식품소재로 이용하고자, 우렁이 콘드로이틴 설페이트 분석 및 고효율 추출방법을 확립하기 위해 본 연구를 수행하였다. 생체의 콘드로이틴 설페이트 함량이 18.4%로 다른 패류(재첩7.1%, 홍합13.5%,다슬기10%)에 비해 높게 나타났다. 추출 전처리 공정으로서 분쇄 및 원적외선 처리 결과, 추출 가공할 경우 원료를 분말화 했을 때 추출 효율이 증가되었으며, 원적외선 처리는 추출에 영향을 미치지 않았다. 추출 보조공정인 극초단파처리와 초음파 추출 방식은 가용성 고형분의 추출은 증가시켰으나 콘드로이틴의 추출 수율은 매우 낮았다. 최적 추출공정 선별을 위해 모든 추출 공정의 가용성 고형분, 단백질 그리고 콘드로이틴 설페이트 추출 수율을 비교한 결과, 가압처리 방식을 이용해 121℃에서 120min간 추출하는 것이 가장 효과적인 것으로 나타났다.
Abstract
Chondroitin sulfate content of a body is 18.4%, which higher than other shellfish(marsh clam 7.1%, mussel 13.5%). This is superior to other shellfish in a functional aspect of target for chondroitin sulfate. The result of pulverization and infrared rays as assisted process extraction was that extract efficiency was increased when material was powdered in case of extract processing. Because the process of infrared rays dose not influence on extract. I thought that you should powder after considering economical efficiency in time of extract processing. When microwave-assisted and ultrasonic waves were extracted as assisted process extraction, both increased extract efficiency but extraction yield of chondroitin sulfate was very low. Although microwave-assisted lowered bioactivity. Ultrasonic showed high bioactivity. Extract efficiency increased in three conditions(an enzyme extract, buffer and autoclaving used distilled water), and chondroitin sulfate was extracted. It was showed that bioactivity was high in a refined article used the very extract solvent. I compared through extraction yield, glycosaminoglycans, bioactivity of all extraction to sort optimum extraction process. Processing 121℃ per 120 min using autoclaving was good in extraction yield, glycosaminoglycans etc. So that I judged autoclaving is optimum extraction process in times of processing in functional food of beverage, concentration solution.
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