Viviparus contectus로부터콘드로이친 황산의 분리 및 화장품적 특성
Isolation and Cosmetic Characterization of Chondroitin Sulfate from Viviparus contectus
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Abstract
Chondroitin sulfate, sulfated glycosaminoglycan(GAG), is an important structural component of cartilage and nowdays also used as a skin conditioning agent. It is obtained usually from animal sources, such as cow cartilage. However, there are some problems of acquisition of chondroitin sulfate from animal for its safety from bovine spongiform encephalopathy(BSE) and its efficient isolation method. In this study, we investigated the nature of chondroitin sulfate isolated from Viviparus contectus and confirmed its skin moisturizing effect. We studied the optimum condition for extracting chondroitin sulfate, cytotoxicity and its activity(cell proliferation , cell migration, collagen synthesis). The optimum extraction condition is under the 121℃ 15lb psi, 2 hours by autoclave and yield is 31.1%. By HPLC-GPC chromatograph, we found that the molecular weight of chodroitin sulfate is 360kDa. chondroitin sulfate showed no cytotoxicity up to 0.1% in human dermal fibroblast and promoted cell proliferation in a dose dependent manner. chondroitin sulfate increased type 1 procollagen synthesis 40% at 1% showing higher activity than L-ascorbic acid. Furthermore in the clinical test to study skin moisturizing effect by corneometer, chondroitin sulfate statistically increased skin moisturizing effect and moisture capacity of skin to 23.4% compared with sodium hyaluronate. These results indicate that chodroitin sulfate isolated from viviparus contectus showed no cytotoxicity, can improve collagen synthesis, cell proliferation and skin moisturizing effect. Therefore, we suggest this chondroitin sulfate to be a functional cosmetic material.
참고문헌 (30)
- Elias, PM, "Epidermal lipids, barrier function, and desquamation", J Invest Dermatol, 80, pp.44~49 (1983)
- J.M Brandner, "Organization and formation of the tight junction system in human epidermis and cultured keratinocytes", Eur. J. Cell boil, 81(5), pp.253~ (2002)
- R. L. Eckert, "The epidermal keratinocytes as a model for the study of gene regulation and cell differentiation", Physiol. Rev., 77, pp.397~ (1997)
- R. Ghadially, "The aged epidermal permeability barrier. Structural, functional, and lipid biochemical abnormalities in humans and a senescent murine model", J Clin Invest, 95(5), pp.2281~2290 (1995)
- M. D. West, "The cellular and molecular biology of skin aging", Arch Dermatol., 130(1), pp.87~ (1994)
- S. Mukherjee, "Retinoids in the treatment of skin aging: An overview of clinical efficacy and safety", Clin Interv Aging, 1(4), pp.327~ (2006)
- S. Pillai, "Ultraviolet radication and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation - induced matrix degradation-a review", Int. J. Cosmet, Science, 27(1), pp.17~ (2005)
- J. L. Mccullough, "Prevention and Treatment of skin aging", Annals of the New York Academy of Science, 1067, pp.323~ (2006)
- J. C. Tilak, "Free raicals and antioxidants in human heaths: Current status and future prospects", J. Association of Physicians of India, 52, pp.796~ (2004)
- S. N. Park, "effect of natural products on skin cells: action and suppression of reactive oxygen species", J.Soc. Cosmet. Scientists Korea, 25(2), pp.77~ (1999)
- Chaqour B, "Chronic UVB- and all-trans retinoic-acid-induced qualitative and quantitative changes in hairless mouse skin", J Photochem Photobiol, 28(2), pp.125~135 (1995)
- Eunsun Jung, "Effect of Camellia japonica oil on human type I procollagen production and skin barrier function", Journal of Ethnopharmacology, 112, pp.127~131 (2007)
- Graeme I. Howling, "The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro", Biomaterials, 22, pp.2959~2966 (2001)
- Singer AJ, "Cutaneous wound healing", New Engl J Med, 2341(10), pp.738~746 (1999)
- Forent R, "Structural and symptomatic efficacy of glucosamine and chondroitin in knee osteoarthritis : A comprehensive meta－analysis", Arch intern Med, 163, pp.1514~1522 (2003)
- F. Stephen, "The extracellular matrix as a biologic scaffold material", j. Biomaterial, 28, pp.3587~3593 (2007)
- Barnhill JG, "Chondroitin product selection for the glucosamine", chondroitin arthritis intervention trial, 2006, pp.46(1), 14~24
- Smetsers TF, "Humansingle-chainantibodiesreactivewithnativec hondroitinsulfatedetectchondroitinsulfatealterationsi nmelanomaandpsoriasis", J. Invest Dermatol. Mar, 122(3), pp.707~716 (2004)
- Perkins, M. E., , Cell, 16, pp.941~952 (1979)
- Forsyth R, "Double blind investigation of the effects of oral supplementation of combined glucosamine hydrochloride (GHCL)and chondroitin sulfate (CS) on stride characteristics of veteran horses", Equine veterinary journal. Supplement, 38(36), pp.622~625 (2006)
- Strong E. E., "Global Diversity of Gastro pods (Gastropoda; Mollusca) in Freshwater", Hydrobiologia, 595, pp.149~166 (2008)
- Volpi N, "Characterizationofalow-sulfatedchondroitinsulfatef romthebodyofViviparusater(molluscagastropoda).Mo dificationofitsstructurebyleadpollution", Glycoconj J, 15(11), pp.1071~1078 (1998)
- Chilkunda D. Nandini, "Novel 70-kDa Chondroitin Sulfate", Dermatan Sulfate Hybrid Chains with a Unique Heterogenous Sulfation Pattern from Shark Skin, Which Exhibit Neuritogenic Activity and Binding Activities for Growth Factors and Neurotrophic Factors, 2005, pp.280(6), 4058~4069
- Richard U. Margolis, "Chondroitin sulfate proteoglycans as mediators of axon growth and path finding", Cell and Tissue Research, 290(2), pp.343~348 (1997)
- Robert J. McKeon, "The Chondroitin Sulfate Proteoglycans Neurocan and Phosphacan Are Expressed by Reactive Astrocytes in the Chronic CNS Glial Scar", The Journal of Neuroscience, 19(24), pp.10778~10788 (1999)
- Jia Ye, "The Chondroitin Sulfate Moiety of Thrombomodulin Binds a Second Molecule of Thrombin", The Journal of Biological chemistry, 4(5), pp.2373~2379 (1993)
- So Yean CHO, "Effects of Low Molecular Weight Chondroitin Sulfate on Type II Collagen-Induced Arthritis in DBA", 1J Mice, 2004, pp.27(1), 47~51
- Joon-Soo Sim, "Evaluation of chondroitin sulfate in shark cartilage powder as a dietary supplement: Raw materials and finished products", Food Chemistry, 101, pp.532~539 (2007)
- Francesca Ronca, "Anti-inflammatory activity of chondroitin sulfate", Osteoarthritis and Cartilage, 6(Supplement A), pp.14~21 (1998)
- KDA Textbook Editing Board, "Dermatology", pp.11~33 (2008), Ryo Moon Gak