한약추출 부산물 첨가가 섬유질 배합사료의 반추위 내 in situ 분해율에 미치는 영향
Effects of Supplementation of Medical Herbal Residues on the in situ Ruminal Degradability of TMR
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본 연구는 한약추출 부산물을 반추가축용 사료로 이용하기 위하여 섬유질배합사료 (TMR)에 첨가하였을 때, 사료영양소의 In situ 반추위내 분해율에 미치는 영향을 구명하고자 수행되었다. 처리구는 대조구로서 한우 비육용 TMR과 TMR에 한약추출 부산물을 5% 첨가한 처리구(Herbal-TMR)로 나누었다. 공시동물은 반추위 캐뉼라가 장착된 Holstein 젖소(체중 650 kg)를 이용하였으며, in situ시험은 5 g의 공시사료를 넣은 nylon bag을 발효시간(0, 3, 6, 9, 12, 24, 48 및 72시간)에 따라 3반복으로 반추위내에서 발효시켰다. 한약추출 부산물의 수분함량은 67%, 조단백질은 9.19%, NDF는 55.4%, ADF는 28.47% 수준이었다. Aflatoxtin과 ochratoxin은 허용범위 이내에 있었고, tannin이 약 0.33% 함유되어 있었다. 건물 소실율은 반추위내 발효 9시간대에서 한약추출 부산물 첨가구가 대조구에 비해 유의적(P<0.05)으로 높았고, 발효 48시간 이후에는 거의 소실되지 않았으며, 건물의 반추위내 유효분해도는 한약추출 부산물 첨가구(Herbal-TMR)가 대조구에 비해 높은 편이었다. 공시사료의 NDF는 한약추출 부산물 첨가구가 대조구에 비해 서서히 분해되는 부분(b)의 시간당 분해속도(c)가 빨라졌으며, ADF는 한약추출 부산물 첨가구에서 ‘b’ 부분이 크게 증가하였지만 시간당 분해속도는 대조구에 비해 크게(1/10) 떨어졌다. 이상의 결과로 볼 때, 본 시험에 사용된 한약추출 부산물은 영양적으로 사료적 가치가 있고 반추가축용 TMR의 원료로서 적합할 것으로 사료된다.
Abstract
The objective of this study was to evaluate the effect of supplementation of medicinal herbal residues on in situ ruminal degradability of total mixed ration (TMR). Treatments were composed of TMR (Control) and TMR supplemented 5% medicinal herbal residues (Herbal-TMR), and rumen-cannulated Holstein cow (650 kg body weight) was used for in situ trial. Nylon bags containing 5 g samples of each feed were incubated in triplicate for 0, 3, 6, 9, 12, 24, 48 and 72 h in the rumen. The contents of moisture, crude protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of medicinal herbal residues were 67%, 9.19%, 55.4% and 28.47%, respectively. Aflatoxtin and ochratoxin as a fungal toxin were in a permissible range, and medicinal herbal residues contained as much as 0.33% tannin. The dry matter (DM) disappearance rate of Herbal-TMR was significantly higher (P<0.05) than that of the control at 9 h incubation, and afterward 48h incubation DM disappearance was a similar level. Effective degradability of DM in the rumen was higher in the Herbal-TMR than the control. In ruminal NDF degradation, the rate (h-1) of degradation of the ‘b’ fraction potentially degraded in rumen (c) was higher in the Herbal-TMR than the control. In ADF, on the contrary, the ‘c’ rate was significantly lower in the Herbal-TMR than the control, even though ‘b’ fraction in the Herbal-TMR was significantly greater than the control. As a result, we concluded that the medicinal herbal residue was suitable as an ingredient of TMR for ruminant.
참고문헌 (28)
- A. O. A. C., "Official methods of analytical chemists", ~ (2005)
- Barry, T. N., "The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 2. Quantitative digestion of carbohydrates and proteins", Br. J. Nutr, 51, pp.493~502 (1984)
- Cheng, X. Y, "Enhanced biogas production from herbal-extraction process residues by microwave-assisted alkaline pretreatment", J. Chem. Technol. Biotechnol, 85, pp.127~131 (2010)
- Dulphy, J. P, "The intake of conserved forage. Forage conservation in the 80'S", Occasional Symposium No. 11. British Grass Sci, pp.107~121 (1979)
- Ehel, F. R., "Influence of particle size on determination of fibrous feed components", J. Dairy Sci, 67, pp.1482~1488 (1984)
- Ehle, F. R., "In situ particle size reduction and the effect of particle size on degradation of crude protein and dry matter in the rumen of dairy steers", J. Dairy Sci, 65, pp.963~971 (1982)
- 황보순, "가시오갈피 지엽의 급여가 흑염소의 생산성, 영양소 소화율 및 혈액성상에 미치는 영향", 한국초지조사료학회지, 26(4), pp.239~248 (2006), 한국초지조사료학회
- Jin, S. K., "Effects of feeding medicinal herbs residue on growth performance, carcass quality and production cost in finishing pigs", Kor. J. Anim. Sci, 41, pp.365~374 (1999)
- 김병기, "한방제재 첨가급여가 한우송아지의 성장, 혈액성상 및 설사에 미치는 영향", 한국동물자원과학회지, 53(5), pp.451~459 (2011), 한국동물자원과학회
- 이희권, "식품 및 사료에서 발생하는 곰팡이독소의 독성 및 대사", Journal of the Korean Society for Applied Biological Chemistry, 45(1), pp.1~10 (2002), 한국응용생명화학회
- Lee, I. D., "Comparison of intake, digestibility and utilization of nitrogen by goats consuming high and low tannin forage sources", J. Korean grassl, 16, pp.230~234 (1996)
- 이신자, "옥수수의 steam flake 처리가 in situ 건물소화율 및 저장기간별 aflatoxin과 ochratoxin 함량에 미치는 영향", 생명과학회지, 18(11), pp.1561~1569 (2008), 한국생명과학회
- Lu, Y., "Interaction of tannin with human salivary proline-rich proteins", Arch. Pral Biol, 43, pp.717~728 (1998)
- Mir, P. S., "Comparison of effective degradability with dry matter degradability measured at mean rumen retention time for several forages and forage : concentrate diets", Anim Feed & Technol, 32, pp.287~296 (1991)
- McLeod, M. N., "Plant tannin- their role in forage utility", Nut. Abstr. Rev, 44, pp.803~815 (1974)
- 문여황, "한약재 추출물이 반추위 in vitro 건물소화율, 휘발성 지방산 생성 및 미생물 성장률에 미치는 영향", 농업생명과학연구, 43(6), pp.67~75 (2009), 농업생명과학연구원
- Naruse, M., "Relationships of dietary and ruminal characteristics to carcass traits in Wagyu steers", Anim. Sci. Technol, 67, pp.146~152 (1996)
- National Research Council., "Nutrient requirements of domestic animals: Nutrient requirements of beef cattle" (1984), NAS-NRC
- Ørskov, E. R., "The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage", J. Agric. Sci, 92, pp.499~503 (1979)
- Ortiz, L. T., "Effects of faba bean tannins on the growth and histological structure of the intestinal tract and liver of chicks and rats", Br. Poult. Sci, 35, pp.743~754 (1994)
- Park, S. J., "Effects of supplemention of Chinese medicine refuse on performance and physiology in broiler chicks", Kor. J. Poult. Sci, 26, pp.195~201 (1999)
- Poppi, D. P., "The validity of the critical size theory for particles leaving the rumen", J. Agric. Sci, 94, pp.275~280 (1980)
- Rheeder, J. P., "Fusarium moniliforme and fumonisins in corn in relation to human esophageal cancer in Transkei", Phytopathology, 82, pp.353~357 (1992)
- SAS., "User's Guide: Statistics. Statistical Analysis System" (1997), Inst. Inc.
- Sung, K. I., "Partial substitution of pellet of residues from oriental herbal medicine in Korean native bulls as a finishing diet", J. Anim. Sci. & Technol, 43, pp.873~880 (2001)
- Van Soest, P. J., "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J. Dairy Sci, 74, pp.3583~3597 (1991)
- Vérité, T., "Révision du système des protéines digestibles dans l'intestin (PDI)", Bull. Tech. CRZV Theix, INRA, 70, pp.19~34 (1987)
- Waghorn, G. C., "The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L", Br. J. Nutr, 57, pp.115~124 (1987)