한약추출 부산물의 반추위 in vitro 소화율, in situ 소실률 및 유효 분해도에 관한 연구
Effects of Herbal Medicine By-products on Ruminal In vitro Degradability, In situ Disappearance, and Effective Degradability
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한약추출 부산물의 영양가치 및 사료 가치를 평가하기 위해 in vitro 소화율 및 in situ 반추위 분해 시험을 하였다. 시험에 사용한 10가지 한약추출 부산물은 인삼(Ginseng radix alba), 백복령(Hoelen), 감초(Glycyrrhizae radix), 당귀(Angelicae gigantis radix), 천궁(Cnidii rhizoma), 작약(Paeoniae radix), 황기(Astragali radix), 생강(Zingiberis rhizoma crudus), 대추(Ziziphi fructus), 진피(Aurantii nobilis pericarpium)로 한약재 중 빈번하게 사용하는 약재이다. 반추위 누관이 장착된 체중이 평균 620kg인 홀스타인 2두를 이용하여 in vitro 소화율 및 in situ 건물 소실률을 평가하였다. 건물 소화율은 한약추출 부산물 3%, 5% 첨가에 따라 유의적으로 증가하였다(P<0.05). 펩신 소화율의 평균은 68.17%로 옥수수, 대두박보다 낮았다. 반추위 건물 소실률은 인삼(Ginseng radix alba), 당귀(Angelicae gigantis radix) 및 대추(Ziziphi fructus)가 유의적으로 높았으며(P<0.05), 백복령(Hoelen), 감초(Glycyrrhizae radix) 및 황기(Astragali radix)는 티모시 건초보다 유의적으로 낮았다(P<0.05). 한약추출 부산물의 12시간과 24시간대 건물 소실률 평균은 38.10, 44.43%이며, 티모시 건초의 평균 건물 소실률인 36.60, 43,25%보다 높았다. 이는 시험에서 사용된 한약추출 부산물은 한약재를 열수 추출 후 사용된 재료로써 한약추출 부산물의 물리적 처리에 의한 영향으로 건물 소실률이 티모시 보다 높은 결과를 보인 것으로 생각된다. 한약추출 부산물의 유효분해도는 인삼(Ginseng radix alba), 천궁(Cnidii rhizoma), 당귀(Angelicae gigantis radix), 대추(Ziziphi fructus) 및 진피(Aurantii nobilis pericarpium)가 높은 추정치를 나타냈다. 결론적으로 한약추출 부산물은 반추동물용 조사료 대체자원으로 이용 가능 할 것으로 생각된다.
Abstract
An in vitro degradation rate and in situ ruminal disappearance trial was conducted to evaluate the nutritional and feed values of herbal medicine by-products (HMB). Major ten materials for preparing oriental herbal medicine, Ginseng radix alba (Panax ginseng C. A. Meyer.), Hoelen (Poria cocos Wolf), Glycyrrhizae radix (Glycyrrhiza uralensis Fisch.), Angelicae gigantis radix (Angrlica gigas Nakai), Cnidii rhizoma (Cnidium officinale Makino), Paeoniae radix (Paeonia lactiflora Pall.), Astragali radix (Astragalus membranaceus Bunge), Zingiberis rhizoma crudus (Zingiberis officinale Roscoe), Ziziphi fructus (Ziziphus jujuba var. inermis Mill.), and Aurantii nobilis pericarpium (Citrus unshiu Marcow.) were used in the experiment. Two ruminally cannulated Holstein dairy cow (average BW 620kg) fed a 60:40 concentrate:timothy diet were used to evaluate in vitro degradation rate and in situ disappearance. Dry matter degradation rates were increased by the addition of 3% and 5% HMB with the statistical differences (P<0.05) in most of the values tested. Average pepsin digestibility (68.17%) of HMB was lower than that of corn and soybean meal. Rumen dry matter disappearance rates of Ginseng radix alba, Angelicae gigantis radix and Ziziphi fructus were significantly (P<0.05) higher, those of Hoelen, Glycyrrhizae radix and Astragali radix were significantly (P<0.05) lower than those of timothy hay. The average DMD of HMB at 12 and 24 h (38.10 and 44.43%) were higher 36.60% and 43.25%, respectively compared to those of timothy hay (Control). Effective rumen degradability calculated with the degradation parameters of Ginseng radix alba, Cnidii rhizoma, Angelicae gigantis radix, Ziziphi fructus and Aurantii nobilis pericarpium was quiet higher than that of control as in the DMD. These results suggest that herbal medicine by-products (HMB) can be used as roughage sources for ruminant animals.
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