농축산미생물배양실 배양효율 및 오염실태 조사
On-site Inspection of Agricultural Microorganism Culture Rooms for Culture Efficiency and Contamination Status
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초록
농축산미생물배양실은 농축산미생물을 지자체에서 안전하게 관리하고 지원하기 위해 설립되었으며,현재 전국에 130개소 이상 분포하고 있다. 주로 배양되는 미생물은 고초균, 광합성균, 유산균, 효모균,질소고정균 등이다. 농축산미생물배양실의 종균관리, 오염등 품질관리에 대한 조사 연구가 수행되지 않아, 본 연구에서 농축산미생물배양실의 질적, 양적 오염 현황을 조사하였다. 조사결과 배양실에서는 공기 중에서 높은 농도의 세균(104CFU/m3)과 진균(104CFU/m3)이 검출되어 오염에 취약하였으며, 또한배양실에서 보유 중인 종균이 다른 균주로 규명되는 경우도 조사 되었다. 효모 종균이 식중독균으로 동정되는 결과도 관찰되었다. 대부분의 농축산미생물배양실에서 미생물의 특성을 고려하지 않고 혼합하여배양하였으며, 기업으로부터 납품받은 일부 배지에서는 미생물이 생육하지 못하는 현상도 조사 되었다. 또한, 인체병원균으로 분류되는 황색포도상구균, Bacillus cereus 병원성대장균등이 농민들에게 나누어주는 미생물배양액에서 검출되었다. 이러한 문제점들은 배양 업무 담당자와 농민, 나아가 국민의 건강을 해치고, 농업 경제에 악영향을 줄 수 있다.
Abstract
Agricultural microorganism culture rooms have been established to provide various microorganismsto farmers. Currently, more than 130 centers which were operated by Agricultural Technology &Extension Center under each local government in nationwide. Major microorganisms, which arecultivated by the culture rooms, are Bacillus, Rhodobacter, Lactobacillus, Saccharomyces,Nitromonas. Contamination during cultivation is a common problem and there is no previousreport available, which inspected the quality and quantity of the culture rooms. Therefore, inthis study we investigated over-all quality of the culture rooms in terms of microbiologicalcontamination. In culture preparation room showed high concentrations rate of bacteria(104CFU/m3) and fungi(104 CFU/m3), which are potentially high risk of contamination, werediscovered. Some long-term stock microorganisms for scale-up cultures were identified differentlydue to contamination. Yeast long-term stock was identified as a food poisoning bacteria. Ingeneral, most of the center mixed and cultured several microorganisms together without consideration of the microorganism’s characteristics. Some of industrial scale media for largescale culture were not able to grow a microorganism. Additionally, human pathogens Staphylococcusaureus and Bacillus cereus, Escherichia coli were contaminated in end-product of scale-upcultures. Our results suggested that serious contamination could adversely affect employee'shealth, farmers’ health and agricultural economy. These problems should be improved throughthe input of microorganism expert or the training of the non-expert public servant.
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