국내 상업용 사료첨가제 생균제의 제품조사 및 품질평가
Survey and Microbiological Quality Control of Commercial Probiotics for Livestock in South Korea
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초록
본 연구는 상업용 사료첨가제 생균제의 제품정보 조사와 미생물학적 평가를 통해 제품개선방안을 모색 하는데 목적이 있다. 상업용 생균제는 무작위로 수집하여 표기균주 및 보증균수 조사, 실제 제품의생균수 및 우점균주 평가, 유통기한 변화에 따른 품질평가, 항생제 내성평가 등을 수행하였다. 제품에표기된 보증 생균수는 평균 6.98 log10 cfu/g, 혼합된 미생물은 평균 2.37개, 주요 미생물은 Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus acidophilus, L. plantarum으로 조사되었다. 실제제품 분석결과 생균수와 표기균주 모두 일치한 제품은 22.22%, 불일치 제품은 24.44%로 분석되었으며주로 검출된 미생물은 S. cerevisiae, B. amyloliquefaciens, B. licheniformis, Enterococcus faecium 으로 나타나 표기정보와 상이한 결과를 보였다. 항생제 감수성 평가결과 클로람페니콜에 대해 저항성이가장 높았으며 암피실린에 민감성이 가장 높았다. 상업용 생균제의 안정성 확보를 위한 자체 품질관리강화와 유통구조 개선이 시급하며 제품의 신뢰성 회복을 위한 효과검증 및 안전성평가에 대해 관리기관의 지속적인 제품 모니터링 및 규정강화가 필요할 것으로 판단된다.
Abstract
This study surveyed and evaluated the product information of commercial probiotics for livestock in South Korea. The products were randomly collected and evaluated for the indicated strains, the number of microorganisms, the date of manufacture, the expiration date, quality of the actual product and the antibiotics resistance. The microorganisms indicated in the product were 34 species of lactic acid bacteria, 33 species of Bacillus sp., 25 species of yeast, and 22 other species. The product with the number of viable cells matched to the indicated strain was 57.78%, the product with the indicated strain was 37.78%, the number of viable cells and indicated strain matched was 22.22%. The information on the microbial strains of the product was different from the actual microbes amount and species identified. Saccharomyces cerevisiae, Bacillus amyloliquefaciens, B. licheniformis, and Enterococcus faecium were the major microorganisms analyzed in the actual product. Highest resistance was in chloramphenicol and susceptible was in ampicillin. In order to restore the reliability of probiotics products and to verify their effectiveness, it is necessary to strengthen regulations of management institutions and improve quality control of producers.
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