Sensitivity Variation of Fusarium oxysporum f. sp. fragariae, Strawberry Wilt Pathogen against Biocontrol Agent
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초록
Strawberry is one of the major economic crops in the modern agriculture industry worldwide. Fusarium wilt disease, caused by the Fusarium oxysporum f. sp. fragariae (FOF), is known as the most problematic factor in strawberry production. In a previous study, Streptomyces griseus S4-7 was isolated from the strawberry rhizosphere, exhibited an exceptional antifungal activity against the Fusarium wilt pathogen. However, sensitivity variation to S4-7 in the pathogen population was not evaluated. Therefore, we collected the pathogen nationwide and screened the sensitivity of FOF to the biological agent. A total of 96 FOF isolates was tested their sensitivity to the S4-7 and less-sensitive FOF isolates had lower cell wall degradation than the standard FOF strain. However, gene expression level of the cell wall organization (pkc1, gcn5) was not different between the less sensitive and the standard FOF strains. The results suggested that among the FOF population, some isolates may develop tolerance against a biocontrol agent through complex tolerance mechanisms.
Abstract
Strawberry is one of the major economic crops in the modern agriculture industry worldwide. Fusarium wilt disease, caused by the Fusarium oxysporum f. sp. fragariae (FOF), is known as the most problematic factor in strawberry production. In a previous study, Streptomyces griseus S4-7 was isolated from the strawberry rhizosphere, exhibited an exceptional antifungal activity against the Fusarium wilt pathogen. However, sensitivity variation to S4-7 in the pathogen population was not evaluated. Therefore, we collected the pathogen nationwide and screened the sensitivity of FOF to the biological agent. A total of 96 FOF isolates was tested their sensitivity to the S4-7 and less-sensitive FOF isolates had lower cell wall degradation than the standard FOF strain. However, gene expression level of the cell wall organization (pkc1, gcn5) was not different between the less sensitive and the standard FOF strains. The results suggested that among the FOF population, some isolates may develop tolerance against a biocontrol agent through complex tolerance mechanisms.
참고문헌 (22)
- Bardin M, "Is the efficacy of biological control against plant diseases likely to be more durable than that of chemical pesticides?", Front Plant Sci, 27, pp.566~ (2015)
- Cha JY, "Microbial and biochemical basis of a Fusarium wilt-suppressive soil", ISME J, 10, pp.119~129 (2016)
- Cho G, "Caryolan-1-ol, an antifungal volatile produced by Streptomyces spp., inhibits the endomembrane system of fungi", Open Biol, 7, pp.170075~ (2017)
- De Cal A, "Effect of chemical fumigation on soil fungal communities in Spanish strawberry nurseries", Appl. Soil Ecol, 28, pp.47~56 (2005)
- Hong SW, "Genome-wide screening antifungal genes in Streptomyces griseus S4-7, a Fusarium wilt disease suppressive microbial agent", FEMS Microbiol Lett, 366, pp.fnz133~ (2019)
- Kim D, "Function and distribution of a lantipeptide in strawberry fusarium wilt disease-suppressive soils", Mol. Plant-Microbe Interact, 32, pp.306~312 (2019)
- 김희경, "Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions", The Plant Pathology Journal, 27(4), pp.301~309 (2011), 한국식물병리학회
- 김지수, "Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP", The Plant Pathology Journal, 33(2), pp.140~147 (2017), 한국식물병리학회
- Kistler HC, "Relatedness of strains of Fusarium oxysporum from crucifers measured by xxamination of mitochondrial and ribosomal DNA", Phytopathology, 77, pp.1289~1293 (1987)
- Koike ST, "Management of Fusarium wilt of strawberry", Crop Prot, 73, pp.67~72 (2015)
- 곽연식, "Take-all of Wheat and Natural Disease Suppression: A Review", The Plant Pathology Journal, 29(2), pp.125~135 (2013), 한국식물병리학회[DOI]
- Levy E, "Resistance mechanisms of Septoria tritici to antifungal products of Pseudomonas", Physiol. Mol. Plant Pathol, 40, pp.163~171 (1992)
- Moon BJ, "Studies on antagonism of Trichoderma species to Fusarium oxysporum f.sp. fragariae V. biological control of Fusarium wilt of strawberry by a mycoparasite, Trichoderma harzianum", Korean J. Plant Pathol, 11, pp.298~303 (1995)
- 남명현, "Biological Control of Strawberry Fusarium Wilt Caused by Fusarium oxysporum f. sp. fragariae Using Bacillus velezensis BS87 and RK1 Formulation", Journal of Microbiology and Biotechnology, 19(5), pp.520~524 (2009), 한국미생물·생명공학회
- Nelson PE, "Fungal wilt diseases of plants", pp.51~80 (1981), Elsevier Inc
- Notz R, "usaric acid-producing strains of Fusarium oxysporum alter 2,4-diacetylphloroglucinol biosynthetic gene expression in Pseudomonas fluorescens CHA0 in vitro and in the rhizosphere of wheat", Appl. Environ. Microbiol, 68, pp.2229~2235 (2002)
- Ownley BH, "Influence of in situ and in vitro pH on suppression of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79", Phytopathology, 82, pp.178~184 (1992)
- Price CL, "Azole fungicides-understanding resistance mechanisms in agriculture fungal pathogens", Pest. Manag. Sci, 71, pp.1054~1058 (2015)
- Schoonbeek HJ, "Fungal ABC transporters and microbial interactions in natural environments", Mol. Plant-Microbe Interact, 15, pp.1165~1172 (2002)
- Schouten A, "Defense responses of Fusarium oxysporum to 2, 4-diacetylphloroglucinol, a broad-spectrum antibiotic produced by Pseudomonas fluorescens", Mol Plant-Microbe Interact, 11, pp.1201~1211 (2004)
- Suga H, "Development of PCR primers to identify Fusarium oxysporum f. sp. fragariae", Plant Dis, 97, pp.619~625 (2013)
- Zhang Y, "Identification and characterization of a Bacillus subtilis strain TS06 as bio-control agent of strawberry replant disease (Fusarium and Verticilium wilts)", Afr. J Biotechnol, 11, pp.570~580 (2012)