파프리카 저장병에 관계하는 미생물상과 병원성검정
The Microflora and Pathogenicity Investigation Related Paprika Postharvest Disease
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초록
본 연구에서는 파프리카 재배포장과 저온저장 후 미생물상을 조사하였으며, 동정된 미생물의 병원성을 검정하여 전염원 유입과 저장병 발생의 관련성을 조사하였다. 재배포장에서는 세균의 경우 과일표면에서 Bacillus spp., Clavibacter sp.와 Pseudomonas spp. 등 7종류의 세균이 분리되었으며 저온저장 후에는 인체에 유해한 균을 포함하여 Erwinia sp., Salmonella spp., Streptococcus spp. 등 8종류의 세균이 분리되었다. 재배포장과 저온저장 후 분리된 주요 진균은 Alternaria sp,, Botrytis sp,, Cladosporium sp,, Fusarium sp,와 Penicillium sp.가 분리되었으며, Geotrichum sp.은 저온저장 후 과일에서 분리되었다. 진균의 경우 분리된 균 모두 파프리카의 부패에 관여하는 것으로 나타났으나 분리된 세균을 단독으로 접종하였을 때 파프리카의 부패에 관여하지 않는 것으로 조사되었다. 이 결과로 진균에 의해 1차적으로 저장병이 발생한 후 세균에 의해 2차적 부패가 발생하는 것으로 판단된다.
Abstract
This study was performed to investigate the relationship between influent inocula and postharvest diseases from paprika of cultivation regions and after low temperature storage period. Seven bacteria were isolated from fruit surfaces collected at paprika cultivation regions and major bacterial genera included Bacillus spp., Clavibacter sp. and Pseudomonas spp. The eight bacterial strains were isolated from five weeks stored fruits at low temperature condition, including potentially harmful bacteria to the human. Five fungal isolates were isolated from either two cultivation or storage period and included Alternaria sp., Botrytis cinerea, Cladosporium sp., Fusarium sp. and Penicillium sp.. Additionally, Geotrichum sp. was isolated only from the low temperature stored fruits. Six of the fungal isolates were concerned as postharvest decay pathogens on the paprika fruit. However, all bacterial isolates were produced any disease symptoms when the bacteria were inoculated on the fruits. As result, it is suggested that the primary postharvest decay may be occurred by fungi and then secondary decay occurred by saprophytic bacteria during storage of the fruit.
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