Learning and Memory Effect of Syringic Acid on Amyloid-β-induced Neurotoxicity in ICR Mice
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To investigate the in vivo cognitive effects of syringic acid(SA), Y-maze and passiveavoidance tests were performed in amyloid-β(Aβ)-induced cytotoxicity. Learning and memoryimpairment by Aβ neurotoxicity was partially recovered in Institute of Cancer Research(ICR)mice orally administered SA(10mg/kg of body weight). The SA treated group showed aninhibitory effect on acetylcholinesterase(AChE) that was extracted from mice brain tissue after invivo tests. Aβ-induced oxidative stress was also examined by malondialdehyde(MDA) and2',7,-dichlorofluorescein diacetate(DCF-DA) assays, and lipid peroxidation of brain homogenatesand cellular oxidative stress were reduced by SA. In cell viability assays using3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT), lactate dehydrogenase(LDH)leakage, and caspase 3/7 activity, the SA treated group showed relatively effective protectionagainst Aβ-induced neurotoxicity compared to the others. Consequently, these results suggest thatSA in black soybean seed coat extract might improve cognitive function because of its neuronalcell protective effects against oxidative stress and the inhibitory effect of AChE as a cholinergicenzyme.
Abstract
To investigate the in vivo cognitive effects of syringic acid(SA), Y-maze and passiveavoidance tests were performed in amyloid-β(Aβ)-induced cytotoxicity. Learning and memoryimpairment by Aβ neurotoxicity was partially recovered in Institute of Cancer Research(ICR)mice orally administered SA(10mg/kg of body weight). The SA treated group showed aninhibitory effect on acetylcholinesterase(AChE) that was extracted from mice brain tissue after invivo tests. Aβ-induced oxidative stress was also examined by malondialdehyde(MDA) and2',7,-dichlorofluorescein diacetate(DCF-DA) assays, and lipid peroxidation of brain homogenatesand cellular oxidative stress were reduced by SA. In cell viability assays using3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT), lactate dehydrogenase(LDH)leakage, and caspase 3/7 activity, the SA treated group showed relatively effective protectionagainst Aβ-induced neurotoxicity compared to the others. Consequently, these results suggest thatSA in black soybean seed coat extract might improve cognitive function because of its neuronalcell protective effects against oxidative stress and the inhibitory effect of AChE as a cholinergicenzyme.
참고문헌 (34)
- Abe K, "Measurement of cellular3-(4, 5-dimethylthiazol-2-yl) - 2 , 5 - diphenyltetrazolium bromide(MTT) reduction activity and lactate dehydrogenase release using MTT", Neurosci. Res, 38, pp.325~329 (2000)
- Preet Anand, "A review on cholinesterase inhibitors for Alzheimer’s disease", Archives of Pharmacal Research, 36(4), pp.375~399 (2013), 대한약학회
- Bryan-Sisneros AA, "Toxic effect of the beta-amyloid precursor protein scolopamin induced hypermolility in rats", Pharm. Pharmacol, 45, pp.841~843 (1993)
- Butterfield DA, "Evidence of oxidative damage in Alzheimer's disease brain: central role for amyloid β-peptide", Trends. Mol. Med, 7, pp.548~554 (2001)
- Chang NS, "Antioxidative effects of green tea power diet against ethanol-induced oxidative damage in rat brain regions", J. Nutr. Health, 34, pp.525~531 (2001)
- Choi SJ, "Ameliorative effect of 1, 2-benzenedicarboxylic acid dinonyl ester against amyloid beta peptide-induced neurotoxicity", Amyloid, 16, pp.15~24 (2009)
- Davis KL, "Tacrine", Lenchet, 345, pp.625~630 (1995)
- Duthie G, "Plant-derived phenolic antioxidants", Curr. Opin. Lipidol, 3, pp.447~451 (2000)
- Ellman GL, "A new and rapid colorimetric determination of acetylcholinesterase activity", Biochem. Pharmacol, 7, pp.88~95 (1961)
- Ferguson, L. R., "Role of plant polyphenols in genomic stability", Mutat. Res, 18, pp.89~111 (2001)
- Gawel S, "Malondialdehyde(MDA) as a lipid peroxidation marker", Wiad. Lek, 59, pp.453~455 (2004)
- Heo HJ, "Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12cells", J. Agr. Food Chem, 53, pp.1984~1989 (2005)
- Heo HJ, "Phenolic phytochemicals in cabbage inhibit amyloid β-protein-induced neurotoxicity", Lebenson. Wiss. Technol, 39, pp.330~336 (2006)
- Isuzugawa K, "Different generation of inhibitors against gallic acid-induced apoptosis produces different sensitivity to gallic acid", Biol. Pharm. Bull, 24, pp.249~253 (2001)
- Jeong CH, "Neuroprotective and anti-oxidant effects of caffeic acid isolated from Erigeron annuus leaf", Chin. Med, 6, pp.25~ (2011)
- Hee Rok Jeong, "Antiamnesic Effects of Ethyl Acetate Fraction from Chestnut (Castanea crenata var. dulcis) Inner Skin on Ab25–35-Induced Cognitive Deficits in Mice", Journal of Medicinal Food, 15(12), pp.1051~1056 (2012), 한국식품영양과학회[DOI]
- 정지희, "Black Soybean Extract Protects Against TMT-Induced Cognitive Defects in Mice", Journal of Medicinal Food, 17(1), pp.83~91 (2014), 한국식품영양과학회[DOI]
- Kim MJ, "Ferulic acid supplementation prevents trimethyltin-induced cognitive deficits in mice", Biosci. Biotechnol. Biochem, 71, pp.1063~1068 (2007)
- Kolodziej H, "Antileishmanial activity of hydrolyzable tannins and their modulatory effects on nitric oxide and tumour necrosis factor-alpha release in macrophages in vitro", Planta. Med, 67, pp.825~832 (2001)
- Lieo A, "Current Pharmacotherapy for Alzheimer's Disease", Annu. Rev. Med, 57, pp.513~533 (2006)
- Miranda S, "The role of oxidative stress in the toxicity induced by amyloid β-peptide in Alzheimer’s disease", Prog. Neurobiol, 62, pp.633~648 (2000)
- Mogana R, "The antiacetylcholinesterase and antileishmanial activities of Canarium patentinervium Miq", Biomed. Res. Int, 2014, pp.903529~903536 (2014)
- Pacheco-Palencia LA, "Chemical composition, antioxidant properties, and thermal stability of a phytochemical enriched oil from Acai(Euterpe oleracea Mart.).", J. Agr. Food Chem, 56, pp.4631~4636 (2008)
- Richardson SJ., "Free radicals in the genesis of Alzheimer's disease", Ann. NY. Acad. Sci, 695, pp.73~76 (1993)
- Rottkamp CA, "Redox-active iron mediates amyloid-β toxicity", Free. Radic. Biol. Med, 30, pp.447~450 (2001)
- Shim JS, "Chemopreventive effects of green tea(Camellia sinensis) among cigarette smokers", Cancer. Epidem. Biomar, 4, pp.387~391 (1995)
- Simon CR, "Programmed cell death in Bradysia hygida(Diptera, Sciaridae) Salivary glands presents apoptotic features", Genesis, 40, pp.22~31 (2004)
- Sramek JJ, "Review of the acetylcholinesterase inhibitor galanthamine", Expert. Opin. Inv. Drug, 9, pp.2393~2402 (2000)
- Trabace L, "Biochemical and neurobeabavioral profile of CHF2819, a novel, orally active acetylcholinesterase inhibitor for Alzheimer's disease", J. Pharmacol. Exp. Ther, 294, pp.187~194 (2000)
- Vegran F, "Implication of alternative splice transcripts of caspase-3 and surviving in chemoresistance", Bull. Cancer, 92, pp.219~226 (2005)
- Wood KW, "The cytoplasmic raf oncogene induces a neuronal phenotype in PC12 cells: a potential role for cellular raf kinases in neuronal growth factor signal transduction", Proc. Natl. Acad. Sci, 90, pp.5016~5020 (1993)
- Wyllie AH, "Cell Death: The significance of apoptosis", Int. Rev. Cytol, 68, pp.251~305 (1980)
- Xu B, "Total Phenolic, phenolic acid, anthocyanin, flavan-3-ol, and flavonol profiles and antioxidant properties of pinto and black beans(Phaseolus vulgaris L.) as affected by thermal processing", J. Agr. Food Chem, 56, pp.7165~7175 (2009)
- Zhao B., "Natural antioxidants protect neurons in Alzheimer’s disease and Parkinson’s disease", Neurochem. Res, 34, pp.630~638 (2009)