Morphological and Molecular Characterization of Sclerotium rolfsii(Teleomorph Athelia rolfsii) Associated with Sclerotium Rot of Cucumis melo L. var. makuwa Makino
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In the present study, a severe outbreak of sclerotium rot on Korean melon(Cucumis melo L. var. makuwa Makino) observed in Jinju, Korea in August of 2014 and 2015 is reported. Thefruit rot disease on the surface of Korean melon fruit appeared as water soaked lesion followedby the development of large rotten areas with abundant white mycelial growth. Abundant whitemycelial mats with globoid and white to brown sclerotia(1-3 mm) were also developed on stemsnear the soil line, and infected plants withered. The fungal pathogen was isolated on potatodextrose agar(PDA) and formed white colonies with numerous sclerotia on PDA. The sclerotiawere globoid in shape, 1-3 mm in size, and white to brown in color. The mycelium formedtypical clamp connection. These measurements and taxonomic characteristics were most similarto those of Sclerotium. DNA sequencing and phylogenetic analysis of the complete internaltranscribed spacer rRNA gene region confirmed that the fungal isolates were Sclerotium rolfsiiSaccardo. Koch's postulates were supported by pathogenicity tests conducted on fruit. Based onmycological characteristics, pathogenicity tests, and molecular identification, the fungus wasidentified as Sclerotium rolfsii. To the best of our knowledge, this is the first report of asclerotium rot caused by S. rolfsii on Korean melon in Korea.
Abstract
In the present study, a severe outbreak of sclerotium rot on Korean melon(Cucumis melo L. var. makuwa Makino) observed in Jinju, Korea in August of 2014 and 2015 is reported. Thefruit rot disease on the surface of Korean melon fruit appeared as water soaked lesion followedby the development of large rotten areas with abundant white mycelial growth. Abundant whitemycelial mats with globoid and white to brown sclerotia(1-3 mm) were also developed on stemsnear the soil line, and infected plants withered. The fungal pathogen was isolated on potatodextrose agar(PDA) and formed white colonies with numerous sclerotia on PDA. The sclerotiawere globoid in shape, 1-3 mm in size, and white to brown in color. The mycelium formedtypical clamp connection. These measurements and taxonomic characteristics were most similarto those of Sclerotium. DNA sequencing and phylogenetic analysis of the complete internaltranscribed spacer rRNA gene region confirmed that the fungal isolates were Sclerotium rolfsiiSaccardo. Koch's postulates were supported by pathogenicity tests conducted on fruit. Based onmycological characteristics, pathogenicity tests, and molecular identification, the fungus wasidentified as Sclerotium rolfsii. To the best of our knowledge, this is the first report of asclerotium rot caused by S. rolfsii on Korean melon in Korea.
참고문헌 (20)
- Stem Rot and Other Diseases Caused by Sclerotium rolfsii, "Aycock R.", North Carolina Agricultural Experiment Station, ~, pp.Tech. Bull. No. 174 (1966)
- "Fungal Databases, Systematic Mycology and Microbiology Laboratory, ARS, USDA" (2014)[URL]
- "Diseases of Korean melon" (2016)[URL]
- Harlton CE, "Genetic diversity in Sclerotium(Athelia) rolfsii and related species", Phytopathology, 85, pp.1269~1281 (1995)
- Kato K, "Genetic characterization of east and south Asian melons, Cucumis melo, by the analysis of molecular polymorphisms and morphological characters", Acta. Hort, 588, pp.217~222 (2001)
- Kitamura S., "Notes on Cucumis of Far East", Acta. Phytotaxon Geobot, 14, pp.41~44 (1950)
- Koike ST, "Vegetable diseases: a color handbook", pp.448~ (2007), Gulf Professional
- The Korean Society of Plant Pathology, "List of plant diseases in Korea" (2009), JY press
- Kwon JH, "First report of sclerotium rot caused by Sclerotium rolfsii on yacón in South Korea", Plant Dis, 98, pp.1443~ (2014)
- Lim TK, "Cucumis melo(Makuwa Group)", pp.219~ (2012), Edible Medicinal and Non-Medicinal Plants
- S. Mahadevakumar, "Morphological and molecular characterization of sclerotium rolfsii associated with fruit rot of Cucurbita maxima", European Journal of Plant Pathology, 145(1), pp.215~219 (2016), Springer Nature[DOI]
- Corticium rolfsii. CMI descriptions of pathogenic fungi and bacteria, "Mordue JE.", Commonwealth Mycological Institute, ~ (1974)
- Okabe I, "Phylogenetic relationship of Sclerotium rolfsii(teleomorph Athelia rolfsii) and S. delphinii based on ITS sequences", Mycol. Res, 107, pp.164~168 (2003)
- Punja ZK., "The biology, ecology and control of Sclerotium rolfsii", Ann. Rev. Phytopathol, 23, pp.97~127 (1985)
- Punja ZK., "Advances in Plant Pathology", pp.523~534 (1988), Academic Press
- Punja ZK, "Comparative growth, morphology and physiology of three sclerotium species", Mycologia, 88, pp.694~706 (1996)
- Punja ZK, "Influence of nutrition, environment and the isolate on basidiocarp formation, development and structure in Athelia(Sclerotium) rolfsii", Mycologia, 74, pp.917~926 (1982)
- Tamura K, "MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0", Mol. Biol. Evol, 24, pp.1596~1599 (2007)
- White TJ, "PCR Protocols: A Guide to Methods and Applications", pp.315~322 (1990), Academic Press, Inc.
- Xu Z, "Phylogenetic placement of plant pathogenic Sclerotium species among teleomorph genera", Mycologia, 102, pp.337~346 (2010)