Athelia rolfsii 에 의한 팥 흰비단병 국내 최초 보고
First Report of Southern Blight caused by Athelia rolfsii on Small Red Bean in Korea
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2023년 경상남도 함안 소재 재배중인 팥의 땅가 줄기에서 흰비단병 증상이 발생하였다. 병징은 팥의 땅가 줄기 아랫부분에 수침상 현상을보이다가 이후 물러지고 부패되어 서서히 시들어 포기 전체가 말라 죽었다. 땅가 줄기에서 병반부와 토양 표면에 흰색의 곰팡이가 발생하였고갈색의 작은 둥근 균핵이 많이 형성되었다. 순수 분리 및 배양을 통해 얻은 곰팡이는 감자한천배지에서 흰색을 띈 매끈한 비단결 균사를 잘 뻗었고균총 위에 갈색의 작은 둥근 균핵을 많이 형성하였다. 균핵의 크기는 1-3 mm이고 균사의 폭은 4-9 µm였다. 또한 균사에서 clamp connection이관찰되었다. 병원성 검정을 통해 최초 포장에서 발견한 동일한 병징이 발달하였고 코흐의 가설에 근거한 재분리를 수행하였다. 곰팡이를 처리하지않은 팥에서는 병징이 발달하지 않았다. 원인 곰팡이의 동정을 명확하게 위해 ITS rRNA 영역 염기서열 및 유전적 유연관계를 분석하였다. 또한종-특이적 PCR 검정을 수행하여 팥에 발생한 흰비단병 증상의 원인 곰팡이를 Athelia rolfsii로 동정하였다. 본 연구는 국내 팥 흰비단병의 최초보고이다. 또한, 흰비단병의 발생은 국내 팥 생산에 큰 위협이 될 수 있을 것이고 본 연구는 향후 팥 흰비단병 방제를 위한 전략 수립에 기초자료로사용되리라 여겨진다.
Abstract
In 2023, symptoms of southern blight occurred on the ground stems of small red beans cultivated at Haman area in South Korea. Symptoms of the disease appear as water-soaking to the lower part of the stem near the ground of the small red bean, which thenbecomes soft and rotted, gradually withers, and the entire plant blight and dies. White mold grew on the lesions on the stems in thesoil line, and many small, brown, round sclerotia were formed. The fungus produced smooth, white, silky hyphae on potato dextroseagar and formed numerous small, brown, round sclerotia. The size of the sclerotia was 1-3 mm, and the width of the hyphae was 4-9µm. Additionally, clamp connections were observed in the hyphae. After artificial inoculation, the same symptoms appeared as thoseoriginally discovered in the field, and re-isolation was conducted according to Koch's postulates. To clearly identify the causative fungus,the ITS rRNA region sequence, phylogenetic relationships, and Athelia rolfsii-specific PCR analysis were performed. The fungus causingsouthern blight in small red beans was identified as Athelia rolfsii. This is the first report of southern blight on small red beans inKorea. In addition, the occurrence of southern blight could pose a significant threat to domestic small red bean production, and this study is expected to be used as fundamental information to establish strategies for controlling southern blight on small red beans inthe future.
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