박과와 가지과 유전자원 종자의 항산화력 및 바이오 활성 화합물 함량 변이
Variation of Antioxidant Activity and Bioactive Compounds Content in Cucurbitaceas and Solanaceae Seeds
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본 연구의 목적은 박과와 가지과 채소 종자들의 항산화력과 총 페놀 및 플라보노이드들의 함량 변이를 조사하여 고 함유 바이오 활성 화합물 채소 유전자원들을 선발하고, 이를 활용한 고부가가치기능성상품 개발을 위한 과학적 기초 자료를 제공하는데 있다. 박과(수박, 호박, 여주와 수세미) 및 가지과(매운 고추, 단고추와 가지) 총 408 유전자원들에 대하여 항산화력과 총 페놀 및 플라보노이드들의 함량을조사하였다. 수박과 호박 종자의 총 페놀 함량은 각각 19.9-343.8 및 6.1-81.2mg·100g-1 DW이며,수박 유전자형의 총 페놀 함량이 160mg·100g-1 DW이상인 유전자원은 12%이었다. 수박과 호박 종자의 플라보노이드 함량의 각각 80 및 41.3mg·100g-1 DW이었다. 매운 고추 종자의 플라보노이드 함량은 64.4-472.5mg·100g-1 DW였고, 평균은 165.0mg·100g-1 DW이었다. 23개의 매운 고추 계통이90% 항산화력이 있는 것으로 분류되었다. 단고추 종자의 항산화력은 35.9-90.3%이며, 항산화력이82%이상인 유전자원은 23%였다. 가지의 총 페놀과 플라보노이드 함량은 각각 38.1-642.0mg·100g-1DW과 14.2-1217.0mg·100g-1 DW이며, 항산화력, 총 페놀 및 플라보노이드 함량이 함께 우수한 8개의 가지 유전자원을 선발하였다. 박과와 가지과 유전자원들의 종자에서 항산화력 및 생리 활성 화합물함량의 변이가 높았다. 그리고 생리 활성 화합물 함량 및 항산화력이 우수하여 선발된 유전자원들의 종자를 활용하여 기능성 부가가치 상품들을 개발하는데 더 효율적으로 활용될 것으로 기대된다.
Abstract
The objectives of this study were to select the seeds of Cucurbitaceae and Solanaceaegenotypes in terms of superior with bioactive compounds content and to inform sophisticateddata for developing the high value-added products. We evaluated to aspects of the antioxidantactivity, polyphenol content, and flavonoid contents in seeds from two vegetable family. Weused in the Cucurbitaceae(watermelon, squash, bitter gourd, and sponge gourd) andSolanaceae(hot pepper, sweet pepper, and egg plant) the total 408 genotypes. In Cucurbitaceae,polyphenol content of watermelon and squash genotypes were ranged 19.9-343.8 and 6.1-81.2mg·100 g-1 DW, respectively. The polyphenol content of watermelon genotypes was 12% amongall genotypes over 160 mg·100 g-1 DW. The mean of flavonoid content in watermelon and squash genotypes represented 80 and 41.3 mg·100 g-1 DW, respectively. In Solanaceae,flavonoid content of hot pepper genotypes was ranged 64.4-472.5 mg·100 g-1 DW, with anaverage of 165.0 mg·100 g-1. The 23 hot pepper genotypes were classified over 90% antioxidantactivity. The antioxidant activity of sweet pepper was ranged 35.9-90.3%, and 23% of allgenotypes represented 82% antioxidant activity. The polyphenol and flavonoid content of eggplant was ranged 38.1-642.0 mg·100 g-1 DW and 14.2-1217.0 mg·100 g-1 DW, respectively. Inaddition, we selected that 8 egg plant with the superior genotypes for antioxidant activity,polyphenol, and flavonoid content. Results revealed that there was significant variation ofantioxidant activity and bioactive compounds contents in both vegetable famaily. In addition, wesuggested that selected genotypes seeds with high contain bioactive compounds will be moreefficiency to develop natural value-added products.
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