박류 단미사료의 사료 유래 대사 단백질 평가
Evaluation of Metabolizable Protein in Commercial Meals
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초록
본 연구는 국내에서 흔히 유통되는 박류 단미사료의 정확한 사료 유래 대사 단백질(metabolizable protein from feed, MPfeed) 함량을 제시하기위해 수행되었다. MPfeed를 측정하기 위해 반추위 미분해 단백질(rumen undegradable protein, RUP) 함량과 RUP의 소장소화율을 평가하였다. 시료 각각의 RUP 함량을 측정하기 위해 홀스타인 거세우 2두를 이용하여 in situ 반추위 분해율 실험을 수행하였다(0, 3, 6, 12, 24, 48, 72,96, 120 h). 각 시료의 소장소화율을 측정하기 위해 12 h in situ 배양을 마친 시료들은 인공 4위액과 인공 소장액에 순차적으로 배양된 후잔량 측정되었다. RUP와 소장 분해율을 측정을 통해 측정된 MPfeed는 전지대두가 45.1%로 가장 높았으며, 국산 대두박(44.3%), 팜박(42.7%),옥수수 주정박(dried distiller’s grains with solubles, DDGS, 40.7%), 수입산 대두박(40.2%), 호마박(39.7%), 야자박(39.3%), 채종박(28.4%),옥 글루텐 밀(26.5%), 옥 배아박(20.5%), 해바라기박(15.3%), 옥 글루텐 피드(14.5%), 아몬드박(5.25%) 순으로 나타났다. 본 연구에서 사용된시료들의 MPfeed는 미국사양표준에서 제시하고 있는 수치와 차이를 나타내었는데, 이는 MPfeed를 측정하기 위해 사용되는 RUP 함량과 RUP의소장소화율에서 차이를 보임에 의한 것으로 추정된다.
Abstract
This study was conducted to provide accurate metabolizable protein from feed (MPfeed) content of commonly distributed protein feedsources in the domestic market. To measure MPfeed, rumen undegradable protein (RUP), and their in vitro small intestine degradability(IVSID) were evaluated. In order to measure the RUP content of each commercial meal, in situ degradability were performed usingtwo Holstein steers (0, 3, 6, 12, 24, 48, 72, 96, 120 h). To measure IVSID, each sample that had been incubated in situ for 12 h,was sequentially incubated in artificial abomasum (pepsin solution), and intestinal fluid (pancreatin, and bile salts). The value of MPfeedwas the highest in whole soybeans (45.1%), followed by domestic soybean meal (44.3%), palm kernel meal (42.7%), dried distiller’sgrains with solubles (40.7%), imported soybean meal (40.2%), sesame meal (39.7%), copra meal (39.3%), rapeseed meal (28.4%), corngluten meal (26.5%), corn germ meal (20.5%), sunflower meal (15.3%), corn gluten feed (14.5%), and almond meal (5.25%). In thisstudy, the MPfeed values showed differences from those presented in NRC. This is presumed to be due to the variations in the RUP,and their SID to measure MPfeed.
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