Effects of Buffer Extraction of Protein Feeds on In Vitro Fermentation Characteristics, Degradation and Methane Production by Rumen Microbes
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The present in vitro study was conducted to examine the effect of buffer solubility of eightprotein feeds (coconut meal, distillers grain, sesame meal, perilla meal, soy source cake, rapeseed meal, soybean meal and lupine) on the fermentation characteristics, degradability of drymatter (DM) and crude protein (CP), and methane (CH4) production by rumen microbes. Bufferextraction increased pH (P<0.05 ~ p<0.001) of the culture solution but tended to lower ammonia-N concentration for all protein feeds. Total volatile fatty acids(VFAs) and each VFAsconcentrations in all incubation was decreased by buffer extraction (P<0.01 ~ P<0.001). Also,molar proportion of acetate in 1h (P<0.001), 3h (P<0.01) and 12h (P<0.05) incubations andmolar proportion of propionate in 1h (P<0.001), 3h (P<0.01), 6h (P<0.05) and 12h (P<0.05)were decreased by buffer extraction. But molar proportion of butyrate in 1h (P<0.001), 3h(P<0.01) and 6h (P<0.05) were increased by buffer extraction. The in vitro effectivedegradability of dry matter (P<0.001) and CP (P<0.001) was decreased by buffer extraction. Themethane production (P<0.01~P<0.001) in all incubation was decreased by buffer extraction. Theresults from in the current study might be useful for diet formulation to improve the feedefficiency of the ruminant animals without massive loss of major nutrients.
Abstract
The present in vitro study was conducted to examine the effect of buffer solubility of eightprotein feeds (coconut meal, distillers grain, sesame meal, perilla meal, soy source cake, rapeseed meal, soybean meal and lupine) on the fermentation characteristics, degradability of drymatter (DM) and crude protein (CP), and methane (CH4) production by rumen microbes. Bufferextraction increased pH (P<0.05 ~ p<0.001) of the culture solution but tended to lower ammonia-N concentration for all protein feeds. Total volatile fatty acids(VFAs) and each VFAsconcentrations in all incubation was decreased by buffer extraction (P<0.01 ~ P<0.001). Also,molar proportion of acetate in 1h (P<0.001), 3h (P<0.01) and 12h (P<0.05) incubations andmolar proportion of propionate in 1h (P<0.001), 3h (P<0.01), 6h (P<0.05) and 12h (P<0.05)were decreased by buffer extraction. But molar proportion of butyrate in 1h (P<0.001), 3h(P<0.01) and 6h (P<0.05) were increased by buffer extraction. The in vitro effectivedegradability of dry matter (P<0.001) and CP (P<0.001) was decreased by buffer extraction. Themethane production (P<0.01~P<0.001) in all incubation was decreased by buffer extraction. Theresults from in the current study might be useful for diet formulation to improve the feedefficiency of the ruminant animals without massive loss of major nutrients.
참고문헌 (19)
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