A Study on an Automatic Multi-Focus System for Cell Observation
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초록
This study is concerned with the mechanism and structure of an optical microscope and an automatic multifocusalgorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cellimages quickly, a z-axis actuator with a resolution of 0.1 μm was designed to control an optical microscopeMoreover, a lighting control system was constructed to select the color and brightness of light that best suit theobject being viewed. Cell images are captured by the instrument and the sharpness of each image is determinedusing Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are appliedto automatically find the most vivid points among multiple images of a single object. A cancer cell imagingexperiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained usingthis microscope. The proposed system is expected to save time and effort required to extract suitable cell imagesand increase the convenience of cell analysis.
Abstract
This study is concerned with the mechanism and structure of an optical microscope and an automatic multifocusalgorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cellimages quickly, a z-axis actuator with a resolution of 0.1 μm was designed to control an optical microscopeMoreover, a lighting control system was constructed to select the color and brightness of light that best suit theobject being viewed. Cell images are captured by the instrument and the sharpness of each image is determinedusing Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are appliedto automatically find the most vivid points among multiple images of a single object. A cancer cell imagingexperiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained usingthis microscope. The proposed system is expected to save time and effort required to extract suitable cell imagesand increase the convenience of cell analysis.
참고문헌 (9)
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